PCR tiling of SARS-CoV-2 virus - Rapid Barcoding Kit 96 V14 and Midnight RT PCR Expansion (SQK-RBK114.96 and EXP-MRT001)

DateVersion numberLanguageDeviceChanges
Sep 17 2024MRT_9186_v114_revG_17Aug24EnglishGridION
17th Sept 2024 - Fixed table formatting in PCR Section of protcocol, step 1.
Sep 17 2024MRT_9186_v114_revG_17Aug24EnglishMinION
17th Sept 2024 - Fixed table formatting in PCR Section of protcocol, step 1.
Sep 17 2024MRT_9186_v114_revG_17Aug24EnglishGridION
17th August 2024 - Fixed table formatting in PCR Section of protcocol, step 1.
Sep 17 2024MRT_9186_v114_revG_17Aug24EnglishMinION
17th August 2024 - Fixed table formatting in PCR Section of protcocol, step 1.
Sep 17 2024MRT_9186_v114_revG_19Apr2023EnglishGridION
02nd July 2024 - Fixed table formatting in PCR Section of protcocol, step 1.
Sep 17 2024MRT_9186_v114_revG_19Apr2023EnglishMinION
02nd July 2024 - Fixed table formatting in PCR Section of protcocol, step 1.
Jul 2 2024MRT_9186_v114_revF_19Apr2023EnglishGridION
02nd July 2024 - Included the following statement to the introduction to the protocol and the Midnight primer sequence information: 'As mutations in SARS-CoV-2 variants emerge amplicon drop out may be observed; for users wishing to design their own primer spike-ins to address this we suggest adding to the appropriate primer pool at a final concentration between 3.33 µM and 6.66 µM.'
Jul 2 2024MRT_9186_v114_revF_19Apr2023EnglishMinION
02nd July 2024 - Included the following statement to the introduction to the protocol and the Midnight primer sequence information: 'As mutations in SARS-CoV-2 variants emerge amplicon drop out may be observed; for users wishing to design their own primer spike-ins to address this we suggest adding to the appropriate primer pool at a final concentration between 3.33 µM and 6.66 µM.'
July 2nd, 2024MRT_9186_v114_revE_19Apr2023EnglishGridion Download 24th January 2024 - Updated recommendations to take 11 µl of eluted DNA library into adapter addition and flow cell loading.
July 2nd, 2024MRT_9186_v114_revE_19Apr2023EnglishMinion Download 24th January 2024 - Updated recommendations to take 11 µl of eluted DNA library into adapter addition and flow cell loading.
January 24th, 2024MRT_9186_v114_revD_19Apr2023EnglishGridion Download 19th September 2023 - Updated flow cell loading instructions to include light shield installation
January 24th, 2024MRT_9186_v114_revD_19Apr2023EnglishMinion Download 19th September 2023 - Updated flow cell loading instructions to include light shield installation
September 19th, 2023MRT_9186_v114_revC_19Apr2023EnglishGridion Download 29th June 2023 - Reformatted the required MinKNOW settings in the "Data acquisition and basecalling" section.
September 19th, 2023MRT_9186_v114_revC_19Apr2023EnglishMinion Download 29th June 2023 - Reformatted the required MinKNOW settings in the "Data acquisition and basecalling" section.
May 3rd, 2023MRT_9186_v114_revB_19Apr2023EnglishGridion Download 3rd May - Updated the Midnight Primer sequences to include the V3 primer spike-ins
May 3rd, 2023MRT_9186_v114_revB_19Apr2023EnglishMinion Download 3rd May - Updated the Midnight Primer sequences to include the V3 primer spike-ins
April 19th, 2023MRT_9186_v114_revA_19Apr2023EnglishGridion Download
April 19th, 2023MRT_9186_v114_revA_19Apr2023EnglishMinion Download