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SARS-CoV-2 virus PCR tiling and sequencing from RNA using EXP-MRT001 and SQK-RBK114.96 (MRT_9186_v114_revJ_23Jun2025)

DateVersionLanguageDeviceChanges
6/23/2025MRT_9186_v114_revJ_23Jun2025EnglishGridION
23 June 2025 - Updated Data acquisition and basecalling section of the protocol to be compatible with updated versions of MinKNOW.
6/23/2025MRT_9186_v114_revJ_23Jun2025EnglishMinION
23 June 2025 - Updated Data acquisition and basecalling section of the protocol to be compatible with updated versions of MinKNOW.
12/13/2024MRT_9186_v114_revH_13Dec2024EnglishGridION
13 December 2024 - Addition of link to MinION Mk1D IT requirements in "Computer requirements and software" and updated link for EPI2ME. - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Addition of MinION Mk1D user manual in "Data acquisition and basecalling", "How to start sequencing". - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/13/2024MRT_9186_v114_revH_13Dec2024EnglishMinION
13 December 2024 - Addition of link to MinION Mk1D IT requirements in "Computer requirements and software" and updated link for EPI2ME. - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Addition of MinION Mk1D user manual in "Data acquisition and basecalling", "How to start sequencing". - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
9/17/2024MRT_9186_v114_revG_17Aug24EnglishGridION
17th Sept 2024 - Fixed table formatting in PCR Section of protcocol, step 1.
9/17/2024MRT_9186_v114_revG_17Aug24EnglishMinION
17th Sept 2024 - Fixed table formatting in PCR Section of protcocol, step 1.
9/17/2024MRT_9186_v114_revG_17Aug24EnglishGridION
17th August 2024 - Fixed table formatting in PCR Section of protcocol, step 1.
9/17/2024MRT_9186_v114_revG_17Aug24EnglishMinION
17th August 2024 - Fixed table formatting in PCR Section of protcocol, step 1.
9/17/2024MRT_9186_v114_revG_19Apr2023EnglishGridION
02nd July 2024 - Fixed table formatting in PCR Section of protcocol, step 1.
9/17/2024MRT_9186_v114_revG_19Apr2023EnglishMinION
02nd July 2024 - Fixed table formatting in PCR Section of protcocol, step 1.
7/2/2024MRT_9186_v114_revF_19Apr2023EnglishGridION
02nd July 2024 - Included the following statement to the introduction to the protocol and the Midnight primer sequence information: 'As mutations in SARS-CoV-2 variants emerge amplicon drop out may be observed; for users wishing to design their own primer spike-ins to address this we suggest adding to the appropriate primer pool at a final concentration between 3.33 µM and 6.66 µM.'
7/2/2024MRT_9186_v114_revF_19Apr2023EnglishMinION
02nd July 2024 - Included the following statement to the introduction to the protocol and the Midnight primer sequence information: 'As mutations in SARS-CoV-2 variants emerge amplicon drop out may be observed; for users wishing to design their own primer spike-ins to address this we suggest adding to the appropriate primer pool at a final concentration between 3.33 µM and 6.66 µM.'
July 2nd, 2024MRT_9186_v114_revE_19Apr2023EnglishGridion Download 24th January 2024 - Updated recommendations to take 11 µl of eluted DNA library into adapter addition and flow cell loading.
July 2nd, 2024MRT_9186_v114_revE_19Apr2023EnglishMinion Download 24th January 2024 - Updated recommendations to take 11 µl of eluted DNA library into adapter addition and flow cell loading.
January 24th, 2024MRT_9186_v114_revD_19Apr2023EnglishGridion Download 19th September 2023 - Updated flow cell loading instructions to include light shield installation
January 24th, 2024MRT_9186_v114_revD_19Apr2023EnglishMinion Download 19th September 2023 - Updated flow cell loading instructions to include light shield installation
September 19th, 2023MRT_9186_v114_revC_19Apr2023EnglishGridion Download 29th June 2023 - Reformatted the required MinKNOW settings in the "Data acquisition and basecalling" section.
September 19th, 2023MRT_9186_v114_revC_19Apr2023EnglishMinion Download 29th June 2023 - Reformatted the required MinKNOW settings in the "Data acquisition and basecalling" section.
May 3rd, 2023MRT_9186_v114_revB_19Apr2023EnglishGridion Download 3rd May - Updated the Midnight Primer sequences to include the V3 primer spike-ins
May 3rd, 2023MRT_9186_v114_revB_19Apr2023EnglishMinion Download 3rd May - Updated the Midnight Primer sequences to include the V3 primer spike-ins
April 19th, 2023MRT_9186_v114_revA_19Apr2023EnglishGridion Download -
April 19th, 2023MRT_9186_v114_revA_19Apr2023EnglishMinion Download -

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