Date | Version | Language | Device | Changes | |
---|---|---|---|---|---|
3/10/2023 | ASFV_9159_v109_revE_18May2022 | English | GridION | February 2023 - Updated product phase to 'Legacy' | |
3/10/2023 | ASFV_9159_v109_revE_18May2022 | English | MinION | February 2023 - Updated product phase to 'Legacy' | |
August 10th, 2022 | ASFV_9159_v109_revD_18May2022 | English | Gridion | Download | 7th July 2022 - A new section has been added to Troubleshooting: "Lot output from PCR" |
August 10th, 2022 | ASFV_9159_v109_revD_18May2022 | English | Minion | Download | 7th July 2022 - A new section has been added to Troubleshooting: "Lot output from PCR" |
June 20th, 2022 | ASFV_9159_v109_revC_18May2022 | English | Gridion | Download | 20th June 2022 - Update the priming and loading the SpotON flow cells for both MinION and GridION with new diagrams. |
June 20th, 2022 | ASFV_9159_v109_revC_18May2022 | English | Minion | Download | 20th June 2022 - Update the priming and loading the SpotON flow cells for both MinION and GridION with new diagrams. |
June 16th, 2022 | ASFV_9159_v109_revB_18May2022 | English | Gridion | Download | 16th June 2022 "Tiled DNA amplification and clean-up": - In the primer pooling instructions, "Primer number" has been changed to "Primer pair". - An additional step has been added: "Immediately before setting up the PCR, dilute each primer pool 1:10 in nuclease-free water to make a 10 μM working stock." - In the step for setting up the PCR reaction, a new note has been added: "We recommend to make a PCR master mix, either for individual primer pools for multiple samples or one for the three primers sets." "DNA repair and end-prep": - After resuspending the bead pellet in 25 µl nuclease-free water, the incubation time has been extended from 2 minutes to 5 minutes. "Adapter ligation and clean-up": - The AMPure XP bead purification step has been changed from 0.5X beads to 0.4X beads (40 μl of AMPure XP beads added to the reaction). |
June 16th, 2022 | ASFV_9159_v109_revB_18May2022 | English | Minion | Download | 16th June 2022 "Tiled DNA amplification and clean-up": - In the primer pooling instructions, "Primer number" has been changed to "Primer pair". - An additional step has been added: "Immediately before setting up the PCR, dilute each primer pool 1:10 in nuclease-free water to make a 10 μM working stock." - In the step for setting up the PCR reaction, a new note has been added: "We recommend to make a PCR master mix, either for individual primer pools for multiple samples or one for the three primers sets." "DNA repair and end-prep": - After resuspending the bead pellet in 25 µl nuclease-free water, the incubation time has been extended from 2 minutes to 5 minutes. "Adapter ligation and clean-up": - The AMPure XP bead purification step has been changed from 0.5X beads to 0.4X beads (40 μl of AMPure XP beads added to the reaction). |
May 18th, 2022 | ASFV_9159_v109_revA_18May2022 | English | Gridion | Download | - |
May 18th, 2022 | ASFV_9159_v109_revA_18May2022 | English | Minion | Download | - |