16th June 2022
"Tiled DNA amplification and clean-up":
- In the primer pooling instructions, "Primer number" has been changed to "Primer pair".
- An additional step has been added: "Immediately before setting up the PCR, dilute each primer pool 1:10 in nuclease-free water to make a 10 μM working stock."
- In the step for setting up the PCR reaction, a new note has been added: "We recommend to make a PCR master mix, either for individual primer pools for multiple samples or one for the three primers sets."
"DNA repair and end-prep":
- After resuspending the bead pellet in 25 µl nuclease-free water, the incubation time has been extended from 2 minutes to 5 minutes.
"Adapter ligation and clean-up":
- The AMPure XP bead purification step has been changed from 0.5X beads to 0.4X beads (40 μl of AMPure XP beads added to the reaction).
16th June 2022
"Tiled DNA amplification and clean-up":
- In the primer pooling instructions, "Primer number" has been changed to "Primer pair".
- An additional step has been added: "Immediately before setting up the PCR, dilute each primer pool 1:10 in nuclease-free water to make a 10 μM working stock."
- In the step for setting up the PCR reaction, a new note has been added: "We recommend to make a PCR master mix, either for individual primer pools for multiple samples or one for the three primers sets."
"DNA repair and end-prep":
- After resuspending the bead pellet in 25 µl nuclease-free water, the incubation time has been extended from 2 minutes to 5 minutes.
"Adapter ligation and clean-up":
- The AMPure XP bead purification step has been changed from 0.5X beads to 0.4X beads (40 μl of AMPure XP beads added to the reaction).