Nature Methods paper shows Direct RNA sequencing
Mon 15th January 2018
A Nature Methods paper, published today, shows that nanopore sequencing can be used to perform direct RNA sequencing, giving real-time sequence data without the need for reverse transcription or amplification steps that are known to introduce biases. Full length, strand-specific RNA sequences can be generated and the method also permits the detection of nucleotide modifications & analogues in RNA.
As well as Direct RNA sequencing kits, Oxford Nanopore also provides cDNA sequencing kits, either using PCR or PCR-Free. Read more about the technology here or see how RNA researchers are using nanopore technology for transcriptome analysis, characterisation of RNA viruses, or more.
To find out more about today's Nature Methods paper and direct RNA sequencing with nanopore technology in general, watch the below interview with Dan Turner, VP Applications at Oxford Nanopore Technologies.