Fig. 3 Comparison of rabbit blood DNA extraction a) read length b) performance summary
There are many extraction kits and protocols available, and these can be used with a wide variety of sample types. The skills required to get good results can differ substantially between kits, as can the purity, integrity and yield of the extracted nucleic acids. As a consequence, some extraction protocols are more suitable for a given downstream analysis than others. For instance, an extraction which yielded shorter fragments of DNA would not be recommended for de novo assembly of a large genome. For each sample type, the selector presents the user with typical fragment-length distributions for all tested extraction methods (Fig. 3a), and one extraction is recommended for the chosen analysis. The user is also presented with performance metrics for the other methods, allowing them to override the recommended choice, if desired.