Alternative transcript isoform detection with single cell and spatial resolution
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Overview
Alternative transcript isoforms have a range of implications in development, health, and disease. However, studying alternative splicing and transcript isoforms has previously been challenging with legacy short-read sequencing technologies, which collapse all isoforms into a single gene expression measurement. Nanopore sequencing can generate reads of any length, enabling characterisation at the isoform level from full-length cDNA strands to identify alternative transcript isoforms with single-cell and spatial resolution.
This application note demonstrates that Oxford Nanopore Technologies, in combination with 10x Genomics cDNA preparation, is a reliable platform to accurately detect alternative transcript isoforms.
In this application note, you will:
- Learn the importance of sequencing full-length cDNA from single cells for high-resolution isoform detection
- Discover the methods and analysis tools used to generate high-quality data
- View our benchmarking data generated using nanopore sequencing
Workflow: single-cell transcriptomics
To learn more about the protocol in this application note, you can download the end-to-end workflow for single-cell transcriptome analysis from 10x Genomics cDNA libraries, using the PromethION device and the EPI2ME analysis platform.
