Obtaining full-length isoforms from single cells with nanopore sequencing

Overview

Characterisation of transcriptomic differences between individual single cells, or spatially across tissues, has provided insight into the mechanisms of cell type-specific diseases, such as neurological disorders and cancer. With nanopore technology, characterise isoform-level expression from full-length cDNAs at the throughput needed to analyse thousands of cells. This end-to-end workflow provides a simple solution for single-cell transcriptome analysis from 10x Genomics cDNA with library preparation in approximately three hours.

In this workflow, you will:

• Find out how full-length cDNA sequencing enhances single-cell transcriptome analysis
• Discover our best practice sequencing workflow in detail, starting from the recommended extraction method, through to primary analysis
• Learn about our recommended sequencing kit and devices