Whole-genome sequencing
Getting started guides
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A guide to microbial sequencing with Oxford Nanopore
A guide to get started with sequencing microbial samples with Oxford Nanopore.
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A guide to human genomics with Oxford Nanopore
This guide introduces how to sequence human genomes with Oxford Nanopore, from the construction of new, highly complete reference assemblies to the comprehensive identification of variants.
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A guide to investigating methylation in the human genome
This guide provides an introduction to the direct sequencing of DNA methylation in human genomes.
Workflow overviews
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Nanopore-only microbial isolate sequencing solution (NO-MISS): flexible, rapid bacterial and fungal isolate whole-genome sequencing
This end-to-end workflow provides a rapid solution for whole-genome Oxford Nanopore sequencing of bacterial and fungal isolates.
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Rapid viral sequencing for genomic surveillance of mpox using metagenomic or targeted approaches
This end-to-end workflow provides guidance for both metagenomic and targeted viral genome sequencing to deliver rapid and actionable results.
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Characterising genomic and epigenomic variation between tumour-normal research samples using long nanopore sequencing reads
This end-to-end workflow overview provides a simple solution for detecting a wide range of tumour-specific variation in a single sequencing assay.
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Rapid identification of pathogenic variants and methylation with whole-genome Oxford Nanopore sequencing
This end-to-end workflow introduces how to perform rapid whole-genome human variant and methylation calling from a blood research sample using high-output DNA sequencing on PromethION 24.
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Assembling high-quality plant genomes with Oxford Nanopore
This end-to-end workflow introduces how to generate a high-quality plant genome assembly from a leaf sample.
Most viewed
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Multiomic nanopore sequencing: revolutionising human disease research
Find out how multiomic nanopore sequencing can accelerate your disease research using a single platform.
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Oxford Nanopore whole-genome sequencing of foodborne pathogens
In this application note, we demonstrate the suitability of Oxford Nanopore sequencing as a standalone platform for foodborne pathogen whole-genome sequencing.
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Oxford Nanopore 24-hour whole-genome sequencing for paediatric rare disease research
Discover how the Oxford Nanopore rapid whole-genome sequencing workflow is delivering fast, actionable results in 24 hours from sample collection.
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EPI2ME: data analysis for all levels of expertise
Discover EPI2ME and how it provides data analysis for all levels of expertise
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High-throughput DNA library preparation with DreamPrep NGS and Ligation Sequencing Kit XL V14 for nanopore sequencing
This application note demonstrates that the Ligation Sequencing Kit V14 (SQK-LSK114) can be automated on the DreamPrep NGS.
Featured resources
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Genomics for a changing planet: sequencing the living world
Discover how a global community of researchers are harnessing Oxford Nanopore sequencing to study the far-reaching impacts of climate change, revealing deep insights across environmental research, agriculture, and pathogen surveillance.
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Accelerating cancer research through comprehensive genomic analysis
Unlock previously hidden variation with nanopore sequencing and characterise cancer at unprecedented resolution.
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Large-cohort cDNA sequencing advances multiomic insights into neurodegenerative disease
In this case study, discover how Kimberley Billingsley and her colleagues at the National Institutes of Health are using Oxford Nanopore large-cohort cDNA sequencing to research the human brain transcriptome, enhancing understanding of the drivers of neurodegenerative diseases.
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From full-length 16S to high-resolution metagenomics: how do I choose the right microbial community workflow for my experiment?
In this masterclass, find the right microbial community sequencing workflow to meet your experimental goals. We’ll cover microbial identification from full-length 16S/ITS sequencing through to comprehensive metagenomic assembly. In this masterclass, discover: • How Oxford Nanopore sequencing deliver
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From speed to scale: how do I choose the right human whole-genome sequencing workflow for my experiment?
In this masterclass, find the right human whole-genome sequencing workflow to meet your experimental goals. We’ll cover whole-genome analysis in just 24 hours through to scaling sequencing for large cohort research. In this masterclass, discover: • How Oxford Nanopore sequencing is advancing human g
Protocols
Ligation sequencing DNA V14 (SQK-LSK114) [ライゲーションシークエンシングDNA V14 (SQK-LSK114) ]
- このプロトコールではゲノムDNAを使用します。
- 調製時間は約６０分です。
- フラグメンテーションは任意としています。
- PCR無し
- R10.4.1フローセルとの互換性
研究用のみ
この翻訳は英語バージョン「X」をもとに翻訳されています。
新しいバージョンが出ている可能性があるので、この翻訳を使用する前にコミュニティーで今の英語のバージョンをご確認ください。
Ultra-Long DNA Sequencing Kit V14 (SQK-ULK114)
Ultra-Long DNA Sequencing Kit V14 (SQK-ULK114)
Analysis workflows
Latest research
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Addressing the global diagnostics gap for childhood leukaemias: a global, multisite type 2 hybrid validation study of nanopore-based Adaptive Sampling whole-genome sequencing
Publication: Addressing the global diagnostics gap for childhood leukaemias: a global, multisite type 2 hybrid validation study of nanopore-based Adaptive Sampling whole-genome sequencing
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Rapid and scalable whole-genome microbial isolate sequencing
Find out how the nanopore-only microbial isolate sequencing solution provides rapid and scalable bacterial isolate sequencing.
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Accelerating precision oncology: Real-time genomic and epigenomic profiling with Oxford Nanopore
Presented at AACR 2026, this recording demonstrates how rapid molecular insights can be leveraged to inform clinical decision-making and advance time-critical cancer care.
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Genome sequencing boosts diagnostic yield for the developmental and epileptic encephalopathies
Publication: Genome sequencing boosts diagnostic yield for the developmental and epileptic encephalopathies
Whole-genome long-read sequencing for rapid comprehensive molecular diagnostics of brain tumours
Publication: Whole-genome long-read sequencing for rapid comprehensive molecular diagnostics of brain tumours
