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Microbial genomics

Getting started guides

Preview of the microbial getting sequencing getting started guideGetting started guide

A guide to microbial sequencing with Oxford Nanopore

A guide to get started with sequencing microbial samples with Oxford Nanopore.


Most viewed

Plot showing the relationship between depth of coverage of nanopore reads and the accuracy of consensus sequences generated by EPI2ME wf-amplicon versus Sanger sequencing.Application note

A successor to Sanger: accurate, full-length, and haplotype-resolved amplicon sequencing with Oxford Nanopore

In this application note, we evaluate Oxford Nanopore sequencing for amplicon analysis using matched datasets generated with both Oxford Nanopore and Sanger sequencing.
Image of metagenomics application noteApplication note

Oxford Nanopore sequencing provides superior metagenome-assembled genome recovery and strain-level resolution from a complex microbiome

In this application note, we demonstrate the capabilities of Oxford Nanopore metagenomics by sequencing and analysing the well-characterised ZymoBIOMICS Fecal Reference.
Application note: bacterial isolate sequencing — 2 pagesApplication note

Oxford Nanopore whole-genome sequencing of foodborne pathogens

In this application note, we demonstrate the suitability of Oxford Nanopore sequencing as a standalone platform for foodborne pathogen whole-genome sequencing.
GridION flyer cover imageBrochure

GridION: one sequencer for many

Made with multiple projects and teams in mind, GridION gives you the freedom to start, stop, and scale your sequencing experiments without impacting others. Meanwhile, onboard compute keeps your real-time data optimised. Discover more.
MinION Mk1D flyer cover imageBrochure

MinION: palm sized, world ready

Discover how the portable yet powerful MinION Mk1D sequencing device can take your insights further afield. Start generating rapid, real-time results without compromise.

Workflow overviews

Preview of the NO-MISS workflow overviewWorkflow overview

Nanopore-only microbial isolate sequencing solution (NO-MISS): flexible, rapid bacterial and fungal isolate whole-genome sequencing

This end-to-end workflow provides a rapid solution for whole-genome Oxford Nanopore sequencing of bacterial and fungal isolates.
Preview of the microbial amplicon barcoding workflow.Workflow overview

Streamlined identification of bacteria, archaea, and fungi with Oxford Nanopore microbial amplicon barcoding

This end-to-end workflow introduces how to sequence the full length 16S gene and ITS region with Oxford Nanopore technology.
Workflow: plasmid sequencing — 2 pagesWorkflow overview

Rapid and complete plasmid characterisation with Oxford Nanopore sequencing

This end-to-end workflow is a rapid method for complete, high-quality whole-plasmid characterisation.
Microbiology stillWorkflow overview

Rapid viral sequencing for genomic surveillance of mpox using metagenomic or targeted approaches

This end-to-end workflow provides guidance for both metagenomic and targeted viral genome sequencing to deliver rapid and actionable results.
Image preview of the pathogen metagenomics workflow overview.Workflow overview

Rapid identification of respiratory pathogens with Oxford Nanopore metagenomics

This end-to-end workflow introduces how to rapidly identify bacterial, fungal, and viral pathogens from respiratory research samples using metagenomic Oxford Nanopore sequencing on a MinION or GridION.

Image of pathogen surveillance white paperWhite paper

Delivering the future of genomic pathogen surveillance with Oxford Nanopore sequencing

Achieve comprehensive, affordable, and real-time analysis of human and animal pathogens with nanopore sequencing.
Image of metagenomics white paperWhite paper

Addressing the challenges of metagenomics with Oxford Nanopore sequencing

Explore how reads with unrestricted length are revealing unprecedented insight into microbial communities.
Oxford Nanopore microbial profilingCase study

High-quality bacterial genomes without the complexity

In this case study, researchers show that near-complete bacterial genomes can be assembled using nanopore sequencing alone.
wastewaterCase study

Wastewater sequencing — an early warning system for infectious disease outbreaks

Monitoring the genomic characteristics of pathogens circulating in a population can reveal important insights into the epidemiological dynamics of an outbreak. Unfortunately, sequencing every confirmed positive sample in a densely populated area is both challenging and expensive. Since viruses are s
Screenshot of Thidathip Wongsurawat testimonialCase study

Testimonial: Thidathip Wongsurawat

Thidathip Wongsurawat is driven to improve patient outcomes through addressing unmet needs. Explore why she is researching the rapid capabilities of nanopore technology to get answers with impact.

Protocols

Microbial Amplicon Barcoding Sequencing for 16S and ITS (SQK-MAB114.24)

Step-by-step protocol for microbial 16S and ITS amplicon sequencing using the Oxford Nanopore Microbial Barcoding Kit.
ONT Logo wheel black

Nanopore-only Microbial Isolate Sequencing Solution (NO-MISS) from cell cultures using SQK-RBK114 (.24 or .96)

Nanopore-only Microbial Isolate Sequencing Solution (NO-MISS) from cell cultures using SQK-RBK114 (.24 or .96)
SQK-RBK110.96 gDNA workflow v1

Rapid Sequencing gDNA Barcoding Protocol (SQK‑RBK114) | Oxford Nanopore Technologies

Learn how to perform rapid genomic DNA barcoding using the Rapid Barcoding Kit V14 (SQK‑RBK114.24 / SQK‑RBK114.96). This fast, high‑yield library preparation workflow enables multiplexing of up to 96 gDNA samples with ~60‑minute prep time and compatibility with R10.4.1 flow cells.

Analysis workflows

wf-metagenomics

This workflow can be used for the taxonomic classification of metagenomic sequencing data.

wf-clone-validation

Among other uses this workflow could determine the success of a molecular cloning experiment and determine whether one DNA sequence has been correctly inserted into another as an experimentalist was expecting.

wf-bacterial-genomes

This workflow is primarily used to assemble genomes from bacterial reads and provide information on features of interest within those assemblies through annotations.

wf-amplicon

This Nextflow workflow provides a simple way to analyse Oxford Nanopore reads generated from haploid amplicons.

wf-alignment

This workflow provides an easy way to align Oxford Nanopore reads and gather mapping stats either locally for small amounts of data or at scale in a distributed environment such as a cluster or the cloud.

Latest research

plasmidWebinar

Unlocking high-resolution, strain-resolved microbiome analyses | ASM Microbe 26

During this video we learn how full length Oxford Nanopore sequencing is unlocking high-resolution microbiome analyses
tuberculosis-stillPublication

Diagnostic value of nanopore-based targeted sequencing technology for subclinical tuberculosis

Publication: Diagnostic value of nanopore-based targeted sequencing technology for subclinical tuberculosis
Microbiology_Metagenomics_STILLS_01Webinar

Understanding bacterial DNA methylation and analysis methods

In this webinar we learn how to advance microbial genomics and epigenomics research with authenticated reference resources.
Microbiology_TuberculosisResearch presentation

Building sustainable pathogen genomic surveillance across Africa

This webinar shares practical lessons from implementing surveillance at scale, to specimen collection and sequencing to bioinformatics and data interpretation. Explore real-world challenges, collaboration models and system-level strategies required to build resilient genomic surveillance.
Poster on rapid whole-genome microbial sequencing, featuring graphs, charts, and text from Oxford Nanopore Technologies.Poster

Rapid and scalable whole-genome microbial isolate sequencing

Find out how the nanopore-only microbial isolate sequencing solution provides rapid and scalable bacterial isolate sequencing.

入門

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