Getting started guides
)
A guide to microbial sequencing with Oxford Nanopore
A guide to get started with sequencing microbial samples with Oxford Nanopore.
Most viewed
)
GridION: one sequencer for many
Made with multiple projects and teams in mind, GridION gives you the freedom to start, stop, and scale your sequencing experiments without impacting others. Meanwhile, onboard compute keeps your real-time data optimised. Discover more.
)
MinION: palm sized, world ready
Discover how the portable yet powerful MinION Mk1D sequencing device can take your insights further afield. Start generating rapid, real-time results without compromise.
)
Oxford Nanopore sequencing solutions for microbiology and infectious disease research
Discover the Oxford Nanopore sequencing techniques that can achieve comprehensive microbial genome characterisation.
Workflow overviews
)
Nanopore-only microbial isolate sequencing solution (NO-MISS): flexible, rapid bacterial and fungal isolate whole-genome sequencing
This end-to-end workflow provides a rapid solution for whole-genome Oxford Nanopore sequencing of bacterial and fungal isolates.
)
Performing accurate species-level bacterial identification with nanopore sequencing
This end-to-end workflow provides a rapid solution for species-level bacterial identification.
Featured resources
)
Genomics for a changing planet: sequencing the living world
Discover how a global community of researchers are harnessing Oxford Nanopore sequencing to study the far-reaching impacts of climate change, revealing deep insights across environmental research, agriculture, and pathogen surveillance.
)
Addressing the challenges of metagenomics with Oxford Nanopore sequencing
Explore how reads with unrestricted length are revealing unprecedented insight into microbial communities.
)
From full-length 16S to high-resolution metagenomics: how do I choose the right microbial community workflow for my experiment?
In this masterclass, find the right microbial community sequencing workflow to meet your experimental goals. We’ll cover microbial identification from full-length 16S/ITS sequencing through to comprehensive metagenomic assembly. In this masterclass, discover: • How Oxford Nanopore sequencing deliver
)
How do I analyse my Oxford Nanopore sequencing data without specialist training?
In this beginner-friendly masterclass, discover how to analyse your Oxford Nanopore sequencing data. Find out how basecalling works, the common file formats you’ll encounter, and how to use MinKNOW to set up and monitor your sequencing run. Then, discover how to analyse your data with EPI2ME — no pr
)
Which library prep workflow is right for my experiment?
Whether you’re sequencing DNA, RNA, or cDNA, a single sample or many in multiplex, there’s an Oxford Nanopore library prep kit for you. In this masterclass, discover how to choose the right one for your workflow. In this masterclass, discover: • The range of Oxford Nanopore library prep solutions av
Protocols
Rapid sequencing DNA - PCR Barcoding Kit 24 V14 (SQK-RPB114.24)
This protocol:
Uses genomic DNA
Has a low input requirement
Has a method involving tagmentation, barcoding and PCR amplification
Allows multiplexing of 1–24 samples
Is compatible with R10.4.1 flow cells
For Research Use Only
Rapid Sequencing gDNA Barcoding Protocol (SQK‑RBK114) | Oxford Nanopore Technologies
Learn how to perform rapid genomic DNA barcoding using the Rapid Barcoding Kit V14 (SQK‑RBK114.24 / SQK‑RBK114.96). This fast, high‑yield library preparation workflow enables multiplexing of up to 96 gDNA samples with ~60‑minute prep time and compatibility with R10.4.1 flow cells.
Ligation sequencing DNA V14 (SQK-LSK114) [ライゲーションシークエンシングDNA V14 (SQK-LSK114) ]
- このプロトコールではゲノムDNAを使用します。
- 調製時間は約60分です。
- フラグメンテーションは任意としています。
- PCR無し
- R10.4.1フローセルとの互換性
研究用のみ
この翻訳は英語バージョン「X」をもとに翻訳されています。
新しいバージョンが出ている可能性があるので、この翻訳を使用する前にコミュニティーで今の英語のバージョンをご確認ください。
Ligation sequencing gDNA - Native Barcoding Kit 24 V14 (SQK-NBD114.24)
This protocol describes how to carry out native barcoding of genomic DNA (gDNA) using the Native Barcoding Kit 24 V14 (SQK-NBD114.24).
Ligation sequencing gDNA - Native Barcoding Kit 96 V14 (SQK-NBD114.96)
This document describes the barcoding of native genomic DNA libraries.
For Research Use Only
Analysis workflows
wf-metagenomics
This workflow can be used for the taxonomic classification of metagenomic sequencing data.
wf-bacterial-genomes
This workflow is primarily used to assemble genomes from bacterial reads and provide information on features of interest within those assemblies through annotations.
wf-amplicon
This Nextflow workflow provides a simple way to analyse Oxford Nanopore reads generated from haploid amplicons.
wf-alignment
This workflow provides an easy way to align Oxford Nanopore reads and gather mapping stats either locally for small amounts of data or at scale in a distributed environment such as a cluster or the cloud.
wf-16s
This workflow can be used for taxonomic classification of single reads from amplicon-targeted sequencing.
Latest research
)
Decoding the Peruvian Amazon with in situ DNA barcoding of vertebrate and plant taxa
Publication: Decoding the Peruvian Amazon with in situ DNA barcoding of vertebrate and plant taxa
)
Barcode 100K specimens: in a single nanopore run
Publication: Barcode 100K specimens: in a single nanopore run
)
Borg extrachromosomal elements of methane-oxidizing archaea have conserved and expressed genetic repertoires
)
Borg extrachromosomal elements of methane-oxidizing archaea have conserved and expressed genetic repertoires
Publications: Borg extrachromosomal elements of methane-oxidizing archaea have conserved and expressed genetic repertoires
)