Getting started guides
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A guide to microbial sequencing with Oxford Nanopore
A guide to get started with sequencing microbial samples with Oxford Nanopore.
Most viewed
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Oxford Nanopore sequencing provides superior metagenome-assembled genome recovery and strain-level resolution from a complex microbiome
In this application note, we demonstrate the
capabilities of Oxford Nanopore metagenomics by
sequencing and analysing the well-characterised
ZymoBIOMICS Fecal Reference.
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GridION: one sequencer for many
Made with multiple projects and teams in mind, GridION gives you the freedom to start, stop, and scale your sequencing experiments without impacting others. Meanwhile, onboard compute keeps your real-time data optimised. Discover more.
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MinION: palm sized, world ready
Discover how the portable yet powerful MinION Mk1D sequencing device can take your insights further afield. Start generating rapid, real-time results without compromise.
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PromethION 2 Integrated: small box, big picture
Explore the PromethION 2 Integrated and bring high-output Oxford Nanopore sequencing to your lab. Run up to two independent flow cells with onboard compute for real-time insights. Discover more.
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Oxford Nanopore sequencing solutions for microbiology and infectious disease research
Discover the Oxford Nanopore sequencing techniques that can achieve comprehensive microbial genome characterisation.
Workflow overviews
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Rapid identification of respiratory pathogens with Oxford Nanopore metagenomics
This end-to-end workflow introduces how to rapidly identify bacterial, fungal, and viral pathogens from respiratory research samples using metagenomic Oxford Nanopore sequencing on a MinION or GridION.
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Performing accurate species-level bacterial identification with nanopore sequencing
This end-to-end workflow provides a rapid solution for species-level bacterial identification.
Featured resources
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Genomics for a changing planet: sequencing the living world
Discover how a global community of researchers are harnessing Oxford Nanopore sequencing to study the far-reaching impacts of climate change, revealing deep insights across environmental research, agriculture, and pathogen surveillance.
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Addressing the challenges of metagenomics with Oxford Nanopore sequencing
Explore how reads with unrestricted length are revealing unprecedented insight into microbial communities.
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Wastewater sequencing — an early warning system for infectious disease outbreaks
Monitoring the genomic characteristics of pathogens circulating in a population can reveal important insights into the epidemiological dynamics of an outbreak. Unfortunately, sequencing every confirmed positive sample in a densely populated area is both challenging and expensive. Since viruses are s
Species-level profiling of environmental microbiota with high-accuracy full-length nanopore 16S sequencing
Rapid bacterial detection plays a vital role in researching microbial ecology. Understanding bacterial composition, diversity, and dynamics is key in the response to pathogen emergence and outbreaks. Sequencing of the 16S ribosomal RNA (rRNA) gene is a predominant method for microbial identification
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NCM 2023 Houston: 2043: a MinION space odyssey
The year is 2043. Following the first human landing on Mars in 2040, NASA has just embarked on the first sustained crewed mission. On the Martian surface, a prefabricated habitat with advanced and sustainable life support systems, shelf-stable food, power and communication infrastructure, and a suit
Protocols
Microbial Amplicon Barcoding Sequencing for 16S and ITS (SQK-MAB114.24)
Step-by-step protocol for microbial 16S and ITS amplicon sequencing using the Oxford Nanopore Microbial Barcoding Kit.
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Nanopore-only Microbial Isolate Sequencing Solution (NO-MISS) from cell cultures using SQK-RBK114 (.24 or .96)
Nanopore-only Microbial Isolate Sequencing Solution (NO-MISS) from cell cultures using SQK-RBK114 (.24 or .96)
Rapid Sequencing gDNA Barcoding Protocol (SQK‑RBK114) | Oxford Nanopore Technologies
Learn how to perform rapid genomic DNA barcoding using the Rapid Barcoding Kit V14 (SQK‑RBK114.24 / SQK‑RBK114.96). This fast, high‑yield library preparation workflow enables multiplexing of up to 96 gDNA samples with ~60‑minute prep time and compatibility with R10.4.1 flow cells.
Analysis workflows
wf-metagenomics
This workflow can be used for the taxonomic classification of metagenomic sequencing data.
wf-bacterial-genomes
This workflow is primarily used to assemble genomes from bacterial reads and provide information on features of interest within those assemblies through annotations.
wf-amplicon
This Nextflow workflow provides a simple way to analyse Oxford Nanopore reads generated from haploid amplicons.
wf-alignment
This workflow provides an easy way to align Oxford Nanopore reads and gather mapping stats either locally for small amounts of data or at scale in a distributed environment such as a cluster or the cloud.
Latest research
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Unlocking high-resolution, strain-resolved microbiome analyses | ASM Microbe 26
During this video we learn how full length Oxford Nanopore sequencing is unlocking high-resolution microbiome analyses
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Sensitive long-read amplicon sequence variant recovery with savont
Publication: Sensitive long-read amplicon sequence variant recovery with savont
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Bioactive molecules unearthed by terabase-scale long-read sequencing of a soil metagenome
Publication: Bioactive molecules unearthed by terabase-scale long-read sequencing of a soil metagenome
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Swine influenza-modified pulmonary microbiota
Publication: Swine influenza-modified pulmonary microbiota
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16S vs shotgun nanopore sequencing detected different taxa in the same freshwater samples
Problem: 16S and shotgun assembly and read-based approaches are widely used for taxonomic profiling. The resulted taxonomic assignments are compared across studies. We showed that the estimated taxa are biased by the choice of shotgun vs amplicon method. Methods: Target database: GTDB Re