New kit extends yields of flow cells
Wed 18th September 2019
Today we've released an updated Flow Cell Wash Kit which will be included in all starter packs and is also available to purchase separately on the Nanopore Store. The kit enables increased yields from existing flow cells.
The Flow Cell Wash Kit provides a highly effective means of removing a library that has been loaded onto a MinION or PromethION flow cell. Once washed, the flow cell can be re-used immediately, or stored for later usage.
Many users have been benefiting from adding a nuclease flush to their workflows, seeing increased throughput from flow cells as a result — the new Flow Cell Wash Kit provides a simple alternative to including a nuclease flush protocol in their workflow.
Minimal sample cross over
The Flow Cell Wash Kit is based on the use of a nuclease to digest and remove nucleic acid that has been loaded onto the flow cell. The washing procedure is very effective with as little as 0.1% of any previously loaded sample contaminating a subsequent run. Barcoding samples will allow the user to filter out any reads from the previous library that is still remaining on the flow cell after the wash.
No impact on read length
After nuclease treatment, and before storing or re-loading the flow cell, the nuclease is inactivated to prevent residual activity impacting future flow cell runs. We do not routinely observe any deterioration in read lengths in sequencing runs performed after the washing procedure (see figure 2).
Improved flow cell output
In experiments where throughput is limited by the increase in pores in the recovering”/“unavailable” state, we have shown that output can be at least doubled by performing several wash steps over the lifetime of a flow cell (see figure 3 and 4).