Nanopore sequencing of native adeno-associated virus vectors for quality control

Overview

Adeno-associated virus (AAV) is a non-enveloped single-stranded DNA virus used in gene therapy that requires accurate validation and quality control (QC) before therapeutic use. Nanopore technology can sequence full-length, native AAV genomes, enabling comprehensive characterisation of the viral genome, including inverted terminal repeats (ITRs), transgenes, and promoters of interest. This end-to-end workflow provides a simple method to characterise full-length native AAV vectors for QC measures.

In this workflow, you will:

• Find out how direct DNA sequencing enhances AAV production validation and QC
• Discover our best practice sequencing workflow in detail, starting from the recommended extraction method, through to primary analysis
• Learn about our recommended sequencing kit and devices