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Oxford Nanopore sequencing of native adeno-associated virus vectors for quality control


Workflow: AAV sequencing — 2 pages

Overview

Adeno-associated virus (AAV) is a non-enveloped single-stranded DNA virus used in gene therapy that requires accurate validation and quality control (QC) before therapeutic use. Nanopore technology can sequence full-length, native AAV genomes, enabling comprehensive characterisation of the viral genome, including inverted terminal repeats (ITRs), transgenes, and promoters of interest.

This end-to-end workflow provides a simple method to characterise full-length, native AAV vectors for QC measures.

In this workflow overview, you will:

  • Find out how direct DNA sequencing enhances AAV production validation and QC
  • Discover our best practice sequencing workflow in detail, starting from the recommended extraction method, through to primary analysis
  • Learn about our recommended sequencing kit and devices

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