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Trans-NanoSim characterizes and simulates nanopore RNA-seq data


We introduce Trans-NanoSim, the first tool that simulates reads with technical and transcriptome-specific features learnt from nanopore RNA-seq data. Through benchmarking on sets of nanopore reads from human and mouse reference transcriptomes, we show the robustness of Trans-NanoSim in capturing the characteristics of nanopore cDNA and direct RNA reads. As a cost-effective alternative to sequencing real transcriptomes, Trans-NanoSim would facilitate the rapid development of analytical tools for nanopore RNA-seq data.

Trans-NanoSim is freely accessible at https://github.com/bcgsc/NanoSim

Authors: Saber Hafezqorani, Chen Yang, Ka Ming Nip, Rene L Warren, Inanc Birol

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