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Simultaneous detection and comprehensive analysis of HPV and microbiome status of a cervical liquid-based cytology sample using Nanopore MinION sequencing


Date: 18th December 2019 | Source: Scientific Reports

Authors: Lili Quan, Ruyi Dong, Wenjuan Yang, Lanyou Chen, Jidong Lang, Jia Liu, Yu Song, Shuiqing Ma, Jialiang Yang, Weiwei Wang, Bo Meng, Geng Tian .

Human papillomavirus (HPV) is a major pathogen that causes cervical cancer and many other related diseases. The HPV infection-related cervical microbiome could be an inducing factor of cervical cancer. However, it is uncommon to find a single test on the market that can simultaneously provide information on both HPV and the microbiome.

Herein, a novel method was developed to simultaneously detect HPV infection and microbiota composition promptly and accurately. It provides a new and simple way to detect vaginal pathogens and also provides valuable information to aid clinical diagnosis. This approach combined multiplex PCR, which targeted both HPV16 E6E7 and full-length 16S rRNA, and nanopore sequencing, to generate enough information to understand the vaginal condition of patients. One HPV-positive liquid-based cytology (LBC) sample was sequenced and analyzed.

After comparing with Illumina sequencing, the results from Nanopore showed a similar microbiome composition. An instant sequencing evaluation showed that 15 min sequencing is enough to identify the top 10 most abundant bacteria. Moreover, two HPV integration sites were identified and verified by Sanger sequencing. This approach has many potential applications in pathogen detection and can potentially aid in providing a more rapid clinical diagnosis.

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