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A guide to sequencing large genomes with Oxford Nanopore

Until recently, large eukaryotic genomes have proved challenging to sequence and assemble with traditional sequencing methods. Producing long and ultra-long reads, high-throughput nanopore sequencing is enhancing large genome assembly, enabling resolution of even the most challenging regions.

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Overview:

Full characterisation of plant and animal genomes via high-throughput sequencing is revolutionising many areas of research. In human and veterinary health, sequencing enables unprecedented insights into disease, whilst investigating genetic variation in plants allows for the maintenance of diversity and selection of desirable traits, such as high yield and resistance to pathogens. Whole-genome sequencing also helps shed light on the evolutionary relationships between organisms.

In this guide, we introduce the different approaches and advantages of sequencing large genomes with Oxford Nanopore technology.

Large genome sequencing guide