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MinION Analysis and Reference Consortium: Phase 1 data release and analysis

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Date: 7th February 2015 | Source: F1000Research

Authors: Camilla Ip, Matthew Loose, John R. Tyson, Mariateresa de Cesare, Bonnie Brown, Miten Jain, Richard Leggett, David Eccles, Vadim Zalunin, John Urban, Paolo Piazza, Rory Bowden, Benedict Paten, Solomon Mwaigwisya, Elizabeth Batty, Jared Simpson, Terrance Snutch, Ewan Birney, David Buck, Sara Goodwin, Hans Jansen, Justin O’Grady, Hugh Olsen.

The advent of a miniaturised DNA sequencing device with a high-throughput contextual sequencing capability embodies the next generation of large scale sequencing tools. The MinION™ Access Programme (MAP) was initiated by Oxford Nanopore Technologies™ in April 2014, giving public access to their USB-attached miniature sequencing device. The MinION Analysis and Reference Consortium (MARC) was formed by a subset of MAP participants, with the aim of evaluating and providing standard protocols and reference data to the community. Envisaged as a multi-phased project, this study provides the global community with the Phase 1 data from MARC, where the reproducibility of the performance of the MinION was evaluated at multiple sites. Five laboratories on two continents generated data using a control strain of Escherichia coli K-12, preparing and sequencing samples according to a revised ONT protocol. Here, we provide the details of the protocol used, along with a preliminary analysis of the characteristics of typical runs including the consistency, rate, volume and quality of data produced. Further analysis of the Phase 1 data presenter here, and additional experiments in Phase 2 of E. coli from MARC are already underway to identify ways to improve and enhance MinION performance

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