London Calling 2022: Update from Oxford Nanopore Technologies
Catch up with the Oxford Nanopore technology update from London Calling 2022 — topics include Kit 14 and the R10.4.1 nanopore for 99.6% simplex & Q30 duplex accuracies, improvements in flow cell output, a new file format, and Clive Brown's, CTO, vision for the future
00:00 Clive Brown introduction
03:45 James Clarke - Platform update
06:40 Move to r10 series
09:00 Improving output
10:00 P2 11:15 Unfinished business
13:20 Flowcell robustness
15:10 Where we are going & new ASIC
19:00 Lakmal - Nanopore update
19:20 Nanopore accuracy occupancy
20:30 Accuracy recap
22:22 Motor and pore improvements
24:00 Kit 14 and r10.4.1 Q20 plus chemistry
24:55 Tuneable run settings
26:59 Duplex - Q30 accuracy
30:00 Long readers & homopolymer sequencing
33:05 Stuart Reid - Data update
36:45 Where are we now - headline accuracy stats
43:13 Summary slide of headline accuracy stata
44:05 Short fragment mode
45:00 Updates to adaptive sampling
46:00 POD5 files
47:22 Dorado
49:00 Rosemary Sinclair Dokos - Product update
49:45 Product features
51:05: Versatility of applications
53:30 Release phases
54:05 Sample prep - ultra-long, cDNA, RNA
57:55 Future applications in pathogen surveillance
58:47 VolTRAX 1:00:00 Kit 14
1:03:32 Release timeline
1:04:20 Summary of key releases
1:09:23 Clive Brown - New and Notable
1:09:45 MinION Mk1D
1:11:30 Accuracy - quality scores
1:12:08 State of the art methylation accuracy
1:13:40 Training base modifications for any modification
1:15:25 Outy sequencing 1:19:25 Short fragment applications
1:23:02 Flow cell loading using an insert for easier loading
1:24:40 Liquid biopsy platform
1:31:15 The future potential for protein sequencing
1:40:00 Summary