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High throughput, error corrected Nanopore single cell transcriptome sequencing

Publication

Date: 7th November 2019 | Source: BioRxiv

Authors: Kevin Lebrigand, Virginie Magnone, Pascal Barbry, Rainer Waldmann.

Droplet-based high throughput single cell isolation techniques tremendously boosted the throughput of single cell transcriptome profiling experiments. However, those approaches only allow analysis of one extremity of the transcript after short read sequencing. We introduce an approach that combines Oxford Nanopore sequencing with unique molecular identifiers to obtain error corrected full length sequence information with the 10xGenomics single cell isolation system. This allows to examine differential RNA splicing and RNA editing at a single cell level.

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