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Rapid sequencing DNA - 16S Barcoding Kit 24 V14 (SQK-16S114.24) (16S_9199_v114_revH_24Nov2025)

DateVersionLanguageDeviceChanges
11/24/202516S_9199_v114_revH_24Nov2025EnglishFlongle
24th November 2025 - In section 1, in the Table under 'Library preparation', the timing for the flow cell priming and loading was changed from 5 min to 10 min and the workflow image was updated to reflect this change. In 'Compatibility of this protocol' MinION Mk1B and MinION Mk1C were removed along with their links for IT requirements as these documents have moved to legacy. This also includes removal of links to their user manuals in 'Data acquisition and basecalling'. - In section 3, 'Library preparation', check your flow cell, the link for the flow cell check was edited. And an important note was added to only use the barcode wells once. - In section 4, 'Flongle Flow Cell loading', the video link for flow cell loading was updated. - In section 4, 'Priming and loading the MinION/GridION Flow Cell', a tip was added to bring the flow cell to room temperature for better visibility of the array. And the addition of BSA to the flow cell priming mix was changed from a 'recommended' to a 'required' instruction. A note was added to the end of this section and for the Flongle Flow Cell loading to explain that when a flow cell is inserted into the MinION Mk1D, there will be a small gap around the sides of the device. - In section 5, 'Data acquisition and basecalling', screenshots were removed for the Flongle device branch and the name of the sequencing kit was specified for the kit selection page in MinKNOW for all device branches.
11/24/202516S_9199_v114_revH_24Nov2025EnglishGridION
24th November 2025 - In section 1, in the Table under 'Library preparation', the timing for the flow cell priming and loading was changed from 5 min to 10 min and the workflow image was updated to reflect this change. In 'Compatibility of this protocol' MinION Mk1B and MinION Mk1C were removed along with their links for IT requirements as these documents have moved to legacy. This also includes removal of links to their user manuals in 'Data acquisition and basecalling'. - In section 3, 'Library preparation', check your flow cell, the link for the flow cell check was edited. And an important note was added to only use the barcode wells once. - In section 4, 'Flongle Flow Cell loading', the video link for flow cell loading was updated. - In section 4, 'Priming and loading the MinION/GridION Flow Cell', a tip was added to bring the flow cell to room temperature for better visibility of the array. And the addition of BSA to the flow cell priming mix was changed from a 'recommended' to a 'required' instruction. A note was added to the end of this section and for the Flongle Flow Cell loading to explain that when a flow cell is inserted into the MinION Mk1D, there will be a small gap around the sides of the device. - In section 5, 'Data acquisition and basecalling', screenshots were removed for the Flongle device branch and the name of the sequencing kit was specified for the kit selection page in MinKNOW for all device branches.
11/24/202516S_9199_v114_revH_24Nov2025EnglishMinION
24th November 2025 - In section 1, in the Table under 'Library preparation', the timing for the flow cell priming and loading was changed from 5 min to 10 min and the workflow image was updated to reflect this change. In 'Compatibility of this protocol' MinION Mk1B and MinION Mk1C were removed along with their links for IT requirements as these documents have moved to legacy. This also includes removal of links to their user manuals in 'Data acquisition and basecalling'. - In section 3, 'Library preparation', check your flow cell, the link for the flow cell check was edited. And an important note was added to only use the barcode wells once. - In section 4, 'Flongle Flow Cell loading', the video link for flow cell loading was updated. - In section 4, 'Priming and loading the MinION/GridION Flow Cell', a tip was added to bring the flow cell to room temperature for better visibility of the array. And the addition of BSA to the flow cell priming mix was changed from a 'recommended' to a 'required' instruction. A note was added to the end of this section and for the Flongle Flow Cell loading to explain that when a flow cell is inserted into the MinION Mk1D, there will be a small gap around the sides of the device. - In section 5, 'Data acquisition and basecalling', screenshots were removed for the Flongle device branch and the name of the sequencing kit was specified for the kit selection page in MinKNOW for all device branches.
11/24/202516S_9199_v114_revH_24Nov2025EspañolGridION
24th November 2025 - In section 1, in the Table under 'Library preparation', the timing for the flow cell priming and loading was changed from 5 min to 10 min and the workflow image was updated to reflect this change. In 'Compatibility of this protocol' MinION Mk1B and MinION Mk1C were removed along with their links for IT requirements as these documents have moved to legacy. This also includes removal of links to their user manuals in 'Data acquisition and basecalling'. - In section 3, 'Library preparation', check your flow cell, the link for the flow cell check was edited. And an important note was added to only use the barcode wells once. - In section 4, 'Flongle Flow Cell loading', the video link for flow cell loading was updated. - In section 4, 'Priming and loading the MinION/GridION Flow Cell', a tip was added to bring the flow cell to room temperature for better visibility of the array. And the addition of BSA to the flow cell priming mix was changed from a 'recommended' to a 'required' instruction. A note was added to the end of this section and for the Flongle Flow Cell loading to explain that when a flow cell is inserted into the MinION Mk1D, there will be a small gap around the sides of the device. - In section 5, 'Data acquisition and basecalling', screenshots were removed for the Flongle device branch and the name of the sequencing kit was specified for the kit selection page in MinKNOW for all device branches.
11/24/202516S_9199_v114_revH_24Nov2025EspañolMinION
24th November 2025 - In section 1, in the Table under 'Library preparation', the timing for the flow cell priming and loading was changed from 5 min to 10 min and the workflow image was updated to reflect this change. In 'Compatibility of this protocol' MinION Mk1B and MinION Mk1C were removed along with their links for IT requirements as these documents have moved to legacy. This also includes removal of links to their user manuals in 'Data acquisition and basecalling'. - In section 3, 'Library preparation', check your flow cell, the link for the flow cell check was edited. And an important note was added to only use the barcode wells once. - In section 4, 'Flongle Flow Cell loading', the video link for flow cell loading was updated. - In section 4, 'Priming and loading the MinION/GridION Flow Cell', a tip was added to bring the flow cell to room temperature for better visibility of the array. And the addition of BSA to the flow cell priming mix was changed from a 'recommended' to a 'required' instruction. A note was added to the end of this section and for the Flongle Flow Cell loading to explain that when a flow cell is inserted into the MinION Mk1D, there will be a small gap around the sides of the device. - In section 5, 'Data acquisition and basecalling', screenshots were removed for the Flongle device branch and the name of the sequencing kit was specified for the kit selection page in MinKNOW for all device branches.
9/18/202516S_9199_v114_revG_01Sep2025EnglishFlongle
01 September 2025 - Included information on primer design and potential limitations to the Troubleshooting section of the protocol
9/18/202516S_9199_v114_revG_01Sep2025EnglishGridION
01 September 2025 - Included information on primer design and potential limitations to the Troubleshooting section of the protocol
9/18/202516S_9199_v114_revG_01Sep2025EnglishMinION
01 September 2025 - Included information on primer design and potential limitations to the Troubleshooting section of the protocol
9/18/202516S_9199_v114_revG_01Sep2025EspañolGridION
01 September 2025 - Included information on primer design and potential limitations to the Troubleshooting section of the protocol
9/18/202516S_9199_v114_revG_01Sep2025EspañolMinION
01 September 2025 - Included information on primer design and potential limitations to the Troubleshooting section of the protocol
9/15/202516S_9199_v114_revG_01Sep2025EnglishFlongle
01 September 2025 - Included information on primer design and potential limitations to the Troubleshooting section of the protocol
9/15/202516S_9199_v114_revG_01Sep2025EnglishGridION
01 September 2025 - Included information on primer design and potential limitations to the Troubleshooting section of the protocol
9/15/202516S_9199_v114_revG_01Sep2025EnglishMinION
01 September 2025 - Included information on primer design and potential limitations to the Troubleshooting section of the protocol
9/15/202516S_9199_v114_revG_01Sep2025EspañolGridION
01 September 2025 - Included information on primer design and potential limitations to the Troubleshooting section of the protocol
9/15/202516S_9199_v114_revG_01Sep2025EspañolMinION
01 September 2025 - Included information on primer design and potential limitations to the Troubleshooting section of the protocol
9/1/202516S_9199_v114_revG_01Sep2025EnglishFlongle
01 September 2025 - Included information on primer design and potential limitations to the Troubleshooting section of the protocol
9/1/202516S_9199_v114_revG_01Sep2025EnglishGridION
01 September 2025 - Included information on primer design and potential limitations to the Troubleshooting section of the protocol
9/1/202516S_9199_v114_revG_01Sep2025EnglishMinION
01 September 2025 - Included information on primer design and potential limitations to the Troubleshooting section of the protocol
9/1/202516S_9199_v114_revG_01Sep2025EspañolGridION
01 September 2025 - Included information on primer design and potential limitations to the Troubleshooting section of the protocol
9/1/202516S_9199_v114_revG_01Sep2025EspañolMinION
01 September 2025 - Included information on primer design and potential limitations to the Troubleshooting section of the protocol
8/26/202516S_9199_v114_revF_26Aug2025EnglishFlongle
26th August 2025 - Changed wording around the barcoding plates in the library preparation section of the protocol to align with both break-away and non-break-away plate formats.
8/26/202516S_9199_v114_revF_26Aug2025EnglishGridION
26th August 2025 - Changed wording around the barcoding plates in the library preparation section of the protocol to align with both break-away and non-break-away plate formats.
8/26/202516S_9199_v114_revF_26Aug2025EnglishMinION
26th August 2025 - Changed wording around the barcoding plates in the library preparation section of the protocol to align with both break-away and non-break-away plate formats.
8/26/202516S_9199_v114_revF_26Aug2025EspañolGridION
26th August 2025 - Changed wording around the barcoding plates in the library preparation section of the protocol to align with both break-away and non-break-away plate formats.
8/26/202516S_9199_v114_revF_26Aug2025EspañolMinION
26th August 2025 - Changed wording around the barcoding plates in the library preparation section of the protocol to align with both break-away and non-break-away plate formats.
8/21/202516S_9199_v114_revE_11Dec2024EnglishFlongle
11th December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
8/21/202516S_9199_v114_revE_11Dec2024EnglishGridION
11th December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
8/21/202516S_9199_v114_revE_11Dec2024EnglishMinION
11th December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
8/21/202516S_9199_v114_revE_11Dec2024EspañolGridION
11th December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
8/21/202516S_9199_v114_revE_11Dec2024EspañolMinION
11th December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
7/22/202516S_9199_v114_revE_11Dec2024EnglishFlongle
11th December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
7/22/202516S_9199_v114_revE_11Dec2024EnglishGridION
11th December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
7/22/202516S_9199_v114_revE_11Dec2024EnglishMinION
11th December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
7/22/202516S_9199_v114_revE_11Dec2024EspañolGridION
11th December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
7/22/202516S_9199_v114_revE_11Dec2024EspañolMinION
11th December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
1/14/202516S_9199_v114_revE_11Dec2024EnglishFlongle
11th December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
1/14/202516S_9199_v114_revE_11Dec2024EnglishGridION
11th December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
1/14/202516S_9199_v114_revE_11Dec2024EnglishMinION
11th December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
1/14/202516S_9199_v114_revE_11Dec2024EspañolFlongle
11th December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
1/14/202516S_9199_v114_revE_11Dec2024EspañolGridION
11th December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
1/14/202516S_9199_v114_revE_11Dec2024EspañolMinION
11th December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
1/14/202516S_9199_v114_revE_11Dec2024EnglishFlongle
11th December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
1/14/202516S_9199_v114_revE_11Dec2024EnglishGridION
11th December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
1/14/202516S_9199_v114_revE_11Dec2024EnglishMinION
11th December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
1/14/202516S_9199_v114_revE_11Dec2024EspañolFlongle
11th December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
1/14/202516S_9199_v114_revE_11Dec2024EspañolGridION
11th December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
1/14/202516S_9199_v114_revE_11Dec2024EspañolMinION
11th December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
1/14/202516S_9199_v114_revE_11Dec2024EnglishFlongle
11th December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
1/14/202516S_9199_v114_revE_11Dec2024EnglishGridION
11th December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
1/14/202516S_9199_v114_revE_11Dec2024EnglishMinION
11th December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/11/202416S_9199_v114_revE_11Dec2024EnglishFlongle
11th December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/11/202416S_9199_v114_revE_11Dec2024EnglishGridION
11th December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/11/202416S_9199_v114_revE_11Dec2024EnglishMinION
11th December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
10/3/202416S_9199_v114_revD_03Oct2024EnglishFlongle
03rd October 2024 - Included specifications that a minimum of 4 samples / barcodes should be used for each library preparation.
10/3/202416S_9199_v114_revD_03Oct2024EnglishGridION
03rd October 2024 - Included specifications that a minimum of 4 samples / barcodes should be used for each library preparation.
10/3/202416S_9199_v114_revD_03Oct2024EnglishMinION
03rd October 2024 - Included specifications that a minimum of 4 samples / barcodes should be used for each library preparation.
10/3/202416S_9199_v114_revD_30Sept2024EnglishFlongle
03rd October 2024 - Included specifications that a minimum of 4 samples / barcodes should be used for each library preparation.
10/3/202416S_9199_v114_revD_30Sept2024EnglishGridION
03rd October 2024 - Included specifications that a minimum of 4 samples / barcodes should be used for each library preparation.
10/3/202416S_9199_v114_revD_30Sept2024EnglishMinION
03rd October 2024 - Included specifications that a minimum of 4 samples / barcodes should be used for each library preparation.
9/30/202416S_9199_v114_revC_30Sept2024EnglishFlongle
30th September 2024 - Moved protocol out of early access phase, removed early access wording.
9/30/202416S_9199_v114_revC_30Sept2024EnglishGridION
30th September 2024 - Moved protocol out of early access phase, removed early access wording.
9/30/202416S_9199_v114_revC_30Sept2024EnglishMinION
30th September 2024 - Moved protocol out of early access phase, removed early access wording.
4/24/202416S_9199_v114_revB_06Dec2023EnglishFlongle
24 April 2024 - Updated introduction to no longer reference 18S or ITS data. Workflow is mainly applicable to 16S data. - Updated nomenclature for EPI2ME (no longer EPI2ME labs).
4/24/202416S_9199_v114_revB_06Dec2023EnglishGridION
24 April 2024 - Updated introduction to no longer reference 18S or ITS data. Workflow is mainly applicable to 16S data. - Updated nomenclature for EPI2ME (no longer EPI2ME labs).
4/24/202416S_9199_v114_revB_06Dec2023EnglishMinION
24 April 2024 - Updated introduction to no longer reference 18S or ITS data. Workflow is mainly applicable to 16S data. - Updated nomenclature for EPI2ME (no longer EPI2ME labs).
April 24th, 202416S_9199_v114_revA_06Dec2023EnglishFlongle Download -
April 24th, 202416S_9199_v114_revA_06Dec2023EnglishGridion Download -
April 24th, 202416S_9199_v114_revA_06Dec2023EnglishMinion Download -

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