From portable to high-throughput benchtop devices, real-time nanopore sensing is a new generation of technology uncovering new biology across multiple sectors.
11th December 2024
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
Jan 14 2025
16S_9199_v114_revE_11Dec2024
English
GridION
11th December 2024
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
Jan 14 2025
16S_9199_v114_revE_11Dec2024
English
MinION
11th December 2024
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
Jan 14 2025
16S_9199_v114_revE_11Dec2024
Español
Flongle
11th December 2024
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
Jan 14 2025
16S_9199_v114_revE_11Dec2024
Español
GridION
11th December 2024
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
Jan 14 2025
16S_9199_v114_revE_11Dec2024
Español
MinION
11th December 2024
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
Jan 14 2025
16S_9199_v114_revE_11Dec2024
English
Flongle
11th December 2024
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
Jan 14 2025
16S_9199_v114_revE_11Dec2024
English
GridION
11th December 2024
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
Jan 14 2025
16S_9199_v114_revE_11Dec2024
English
MinION
11th December 2024
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
Jan 14 2025
16S_9199_v114_revE_11Dec2024
Español
Flongle
11th December 2024
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
Jan 14 2025
16S_9199_v114_revE_11Dec2024
Español
GridION
11th December 2024
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
Jan 14 2025
16S_9199_v114_revE_11Dec2024
Español
MinION
11th December 2024
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
Jan 14 2025
16S_9199_v114_revE_11Dec2024
English
Flongle
11th December 2024
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
Jan 14 2025
16S_9199_v114_revE_11Dec2024
English
GridION
11th December 2024
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
Jan 14 2025
16S_9199_v114_revE_11Dec2024
English
MinION
11th December 2024
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
Dec 11 2024
16S_9199_v114_revE_11Dec2024
English
Flongle
11th December 2024
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
Dec 11 2024
16S_9199_v114_revE_11Dec2024
English
GridION
11th December 2024
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
Dec 11 2024
16S_9199_v114_revE_11Dec2024
English
MinION
11th December 2024
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
Oct 3 2024
16S_9199_v114_revD_03Oct2024
English
Flongle
03rd October 2024
- Included specifications that a minimum of 4 samples / barcodes should be used for each library preparation.
Oct 3 2024
16S_9199_v114_revD_03Oct2024
English
GridION
03rd October 2024
- Included specifications that a minimum of 4 samples / barcodes should be used for each library preparation.
Oct 3 2024
16S_9199_v114_revD_03Oct2024
English
MinION
03rd October 2024
- Included specifications that a minimum of 4 samples / barcodes should be used for each library preparation.
Oct 3 2024
16S_9199_v114_revD_30Sept2024
English
Flongle
03rd October 2024
- Included specifications that a minimum of 4 samples / barcodes should be used for each library preparation.
Oct 3 2024
16S_9199_v114_revD_30Sept2024
English
GridION
03rd October 2024
- Included specifications that a minimum of 4 samples / barcodes should be used for each library preparation.
Oct 3 2024
16S_9199_v114_revD_30Sept2024
English
MinION
03rd October 2024
- Included specifications that a minimum of 4 samples / barcodes should be used for each library preparation.
Sep 30 2024
16S_9199_v114_revC_30Sept2024
English
Flongle
30th September 2024
- Moved protocol out of early access phase, removed early access wording.
Sep 30 2024
16S_9199_v114_revC_30Sept2024
English
GridION
30th September 2024
- Moved protocol out of early access phase, removed early access wording.
Sep 30 2024
16S_9199_v114_revC_30Sept2024
English
MinION
30th September 2024
- Moved protocol out of early access phase, removed early access wording.
Apr 24 2024
16S_9199_v114_revB_06Dec2023
English
Flongle
24 April 2024
- Updated introduction to no longer reference 18S or ITS data. Workflow is mainly applicable to 16S data.
- Updated nomenclature for EPI2ME (no longer EPI2ME labs).
Apr 24 2024
16S_9199_v114_revB_06Dec2023
English
GridION
24 April 2024
- Updated introduction to no longer reference 18S or ITS data. Workflow is mainly applicable to 16S data.
- Updated nomenclature for EPI2ME (no longer EPI2ME labs).
Apr 24 2024
16S_9199_v114_revB_06Dec2023
English
MinION
24 April 2024
- Updated introduction to no longer reference 18S or ITS data. Workflow is mainly applicable to 16S data.
- Updated nomenclature for EPI2ME (no longer EPI2ME labs).