Resources: Documentation

This protocol uses N50 of 30 kb genomic DNA extracted from human cell lines * Sample preparation time: ~320 minutes and library preparation time: ~90 minutes * Data analysis: 1-2 hours * No PCR * Compatible with R10.4.1 flow cells For Research Use Only

This protocol uses N50 of 10 kb genomic DNA extracted from human cell lines - Sample preparation time: ~220 minutes and library preparation time: ~90 minutes - Data analysis: 1-2 hours - No PCR - Compatible with R10.4.1 flow cells For Research Use Only

Yeast DNA

Yeast DNA (Community-developed)

Mycobacterium tuberculosis DNA

SPRI size selection protocol for >1.5-2 kb DNA fragments

Soil DNA

Removal of short fragments with the Short Fragment Eliminator Expansion (EXP-SFE001)

Human blood cell-free DNA (cfDNA) extraction for singleplex sequencing

Human cell line RNA

Plasmid DNA extraction method

Fruit fly (Drosophila melanogaster) RNA

Button mushroom (Agaricus bisporus) DNA

Human blood cell-free DNA (cfDNA) extraction for multiplex sequencing

Reptile tissue DNA

Human saliva DNA

Alpha and beta globin mRNA depletion from total RNA extracted from human blood

Human blood RNA

High output, long read sequencing from human blood

Gram-negative bacterial DNA

Western clawed frog (Xenopus tropicalis) muscle DNA

Influenza A RNA

FFPE human cell DNA

Environmental water (eDNA)

Automated gDNA extraction from dry blood spots (FTA cards)

Fruit fly (Drosophila melanogaster) DNA

Whole genome colony PCR

Worm (Caenorhabditis elegans) RNA

Worm (Caenorhabditis elegans) DNA

Extracting and preparing DNA from brain tissue for Oxford Nanopore Sequencing

Arabidopsis (Arabidopsis thaliana LEr) leaf RNA

Agarose plug DNA

3’ polyadenylation of RNA transcripts using E. coli poly(A) polymerase (PAP)

Yeast cell culture RNA

Stool pellet gDNA

Salmon sample gDNA

Rabbit skin gDNA

Rabbit muscle gDNA

Rabbit lung gDNA

Rabbit fat gDNA

Rabbit blood gDNA

Plant leaf gDNA

Mammalian sample gDNA

Gram-positive bacterial gDNA

Fish gDNA

Methods for enrichment of polyadenylated RNA molecules

Human cell line gDNA

Avian liver gDNA

Avian gDNA

Avian blood gDNA