Enrichment of polyadenylated RNA molecules
Extraction Method Group
Enrichment of polyadenylated RNA molecules
These protocols describe two methods for the enrichment of polyadenylated RNA molecules from extracted total RNA by: removal of rRNA using the RiboMinus™ Eukaryotic Kit v2, or selectively isolating poly(A) molecules using the DYNAL™ Dynabeads TM mRNA Purification Kit. To demonstrate the performance, RNA was extracted from Saccharomyces cerevisiae cells (S288c) and GM12878 cells using TRIzol based methods: sequencing libraries were prepared from 1000 ng total RNA or 500 ng poly(A)-enriched RNA using the Direct RNA Sequencing Kit and run on MinION.
FOR RESEARCH USE ONLY
These protocols describe two methods for the enrichment of polyadenylated RNA molecules from extracted total RNA by: removal of rRNA using the RiboMinus™ Eukaryotic Kit v2, or selectively isolating poly(A) molecules using the DYNAL™ Dynabeads TM mRNA Purification Kit. To demonstrate the performance, RNA was extracted from Saccharomyces cerevisiae cells (S288c) and GM12878 cells using TRIzol based methods: sequencing libraries were prepared from 1000 ng total RNA or 500 ng poly(A)-enriched RNA using the Direct RNA Sequencing Kit and run on MinION.