Resources: Documentation

The effect of varying the number of PCR cycles in the PCR-cDNA Sequencing Kit

Telomere-to-telomere sequencing (T2T) know-how document

Stability of SPRI size selection beads

Short Fragment Eliminator Kit (EXP-SFE001) know-how document

Direct-from-colony microbial sequencing: rapid Salmonella serotyping know-how and info sheet

RNA stability

RNA Integrity Number (RIN)

Rapid metagenomic sequencing for surveillance of bacterial, fungal and viral pathogens using SQK-RPB114.24 know-how document

Polyadenylation of non-poly(A) transcripts using E. coli poly(A) polymerase

Accurate poly(A) tail length estimation for Direct RNA Sequencing (SQK-RNA002) and cDNA (SQK-PCS111, SQK-PCB111.24) sequencing kits

Ligation Sequencing sample input and flow cell loading know-how

DNA library stability - Ligation Sequencing Kit

Flow Cell Wash Kit (EXP-WSH004 or EXP-WSH004-XL) know-how

Enrichment of polyadenylated RNA molecules know-how document

DNA stability - storage

DNA stability - freeze-thawing

Chromatin accessibility know-how document

Updated method for cell-free DNA (cfDNA) methylation profiling

Optimisation of library preparation for longer cell-free DNA (cfDNA) libraries

Assessment of cfDNA extraction kits for nanopore sequencing

Comparative analysis of cfDNA collection tubes for nanopore sequencing

Telo-Seq know-how document

A method to remove amplified cDNA artefact in SQK-PCS111 and SQK-PCB111.24 libraries

Sequencing products of multiple displacement amplification (MDA)

A method to remove highly abundant globin transcripts from human blood RNA samples

Adeno-associated virus (AAV) sequencing - development notes and best practices

This protocol is for recovering DNA libraries from flow cells for continued sequencing - Compatible with both MinION and PromethION flow cells, and all DNA sequencing kits For Research Use Only

For Research Use Only

Size selection

Optional fragmentation of gDNA

Chemistry Technical Document