Rapid and complete plasmid characterisation with Oxford Nanopore sequencing

Plasmids are the backbone of molecular biology, playing a pivotal role in applications such as gene therapy and vaccine development, genetic engineering, industrial biotechnology, and basic scientific research. Oxford Nanopore sequencing enables the highly accurate, flexible, and secure characterisation of full-length plasmid sequences in-house without the need for primers, with results obtained in hours — negating the need to send constructs to third parties for validation

This end-to-end workflow is a rapid method for complete, high-quality whole-plasmid characterisation.

In this workflow overview, you will:

• Find out how full-length plasmid sequencing enhances construct validation, including confirmation of sequence identity
• Discover our best practice sequencing workflow in detail, starting from the recommended extraction method, through to primary data analysis
• Learn about our recommended library prep kit and sequencing devices