Long reads reveal small scale genome structural variations in Allo-tetraploid Canola
)
Genomic structural variation (SV) in the form of insertions, deletions or translocations have been shown to contribute to multiple important traits in oilseed rape (Brassica napus), such as disease resistance and flowering time. However, effective detection of gene-sized SV events is limited by the read length of second-generation sequencing technologies. Recent advances in long-read sequencing based on Oxford Nanopore technologies provide new opportunities to greatly expand our understanding of the influence of gene scale SV on key phenotypic traits. Here we identified widespread gene scale SV in the rapeseed genome. We also discovered genomic rearrangements associated with flowering time and disease resistance in the rapeseed genome.