Dancing the Nanopore Limbo: Low DNA input for successful microbial metagenomics with Oxford Nanopore Technologies

Short-read assemblies often result in incomplete and/or highly fragmented metagenome-assembled genomes (MAGs)

• Nanopore long reads greatly improve the assembly quality of MAGs

• Recommended DNA quantity usually exceeds the recoverable amount of DNA isolated from environmental samples

⇒ Evaluation of lower-than-recommended DNA quantities for Nanopore library preparation by determining sequencing quality, community composition, assembly quality and recovery of MAG