Alternative transcript isoform detection with single cell and spatial resolution
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- Alternative transcript isoform detection with single cell and spatial resolution
Overview
Alternative transcript isoforms have a range of implications in development, health, and disease. Studying alternative splicing and transcript isoforms, however, has been challenging for several reasons. One such difficulty is the common use of bulk sequencing, which only provides an average of the transcripts expressed across a population of cells. Another limitation has been the use of short-read sequencing methods, which lose key information from full-length transcripts. To unambiguously identify which isoforms are expressed, entire transcripts must be sequenced intact.
Current single cell gene expression library preparation methods fragment the cDNA to be compatible with shortread sequencers. These technologies also tend to bias captured transcripts to either the 5’ or 3’ ends. However, by combining single cell assays with long sequencing reads, it is now possible to identify alternative transcript isoforms with single cell resolution.
Workflow: single-cell transcriptomics
To learn more, you can also download a complete workflow for single-cell transcriptome analysis from 10x Genomics cDNA libraries, using the PromethION device, by clicking here.