PCR tiling of SARS-CoV-2 virus - classic protocol (SQK-LSK109 with EXP-NBD196)

DateVersion numberLanguageDeviceChanges
Mar 10 2023PTCN_9103_v109_revR_13Jul2020EnglishGridION
February 2023 - Updated product phase to 'Legacy'
Mar 10 2023PTCN_9103_v109_revR_13Jul2020EnglishMinION
February 2023 - Updated product phase to 'Legacy'
August 10th, 2022PTCN_9103_v109_revQ_13Jul2020EnglishGridion Download 14th July 2022 - Updated compatibilities and removed references to discontinued flow cells
August 10th, 2022PTCN_9103_v109_revQ_13Jul2020EnglishMinion Download 14th July 2022 - Updated compatibilities and removed references to discontinued flow cells
June 30th, 2022PTCN_9103_v109_revP_13Jul2020EnglishGridion Download 30th June 2022 - Update the priming and loading the SpotON flow cells for both MinION and GridION with new diagrams.
June 30th, 2022PTCN_9103_v109_revP_13Jul2020EnglishMinion Download 30th June 2022 - Update the priming and loading the SpotON flow cells for both MinION and GridION with new diagrams.
April 13th, 2022PTCN_9103_v109_revO_13Jul2020EnglishGridion Download 14th April 2022 Protocol name change
April 13th, 2022PTCN_9103_v109_revO_13Jul2020EnglishMinion Download 14th April 2022 Protocol name change
October 11th, 2021PTCN_9103_v109_revN_13Jul2020EnglishGridion Download 11th Oct 2021 - Flow cell loading recommendations have been clarified
October 11th, 2021PTCN_9103_v109_revN_13Jul2020EnglishMinion Download 11th Oct 2021 - Flow cell loading recommendations have been clarified
September 14th, 2021PTCN_9103_v109_revM_13Jul2020EnglishGridion Download 14th Sept 2021 - In "Overview of the protocol" and "Equipment and consumables", there is a note that the 96 barcode orientation on the plate differs depending on the kit batch number
September 14th, 2021PTCN_9103_v109_revM_13Jul2020EnglishMinion Download 14th Sept 2021 - In "Overview of the protocol" and "Equipment and consumables", there is a note that the 96 barcode orientation on the plate differs depending on the kit batch number
September 13th, 2021PTCN_9103_v109_revL_13Jul2020EnglishGridion Download 13th Sept 2021 - Added tables of kit contents in the Equipment and Consumables section.
September 13th, 2021PTCN_9103_v109_revL_13Jul2020EnglishMinion Download 13th Sept 2021 - Added tables of kit contents in the Equipment and Consumables section.
August 6th, 2021PTCN_9103_v109_revK_13Jul2020EnglishGridion Download 16th June 2021 - Required settings in MinKNOW in the 'Sequencing and data analysis' section has been updated include instructions to toggle 'Override mid barcoding score' and to increase minimum mid barcoding score from 40 to 50.
August 6th, 2021PTCN_9103_v109_revK_13Jul2020EnglishMinion Download 16th June 2021 - Required settings in MinKNOW in the 'Sequencing and data analysis' section has been updated include instructions to toggle 'Override mid barcoding score' and to increase minimum mid barcoding score from 40 to 50.
April 28th, 2021PTCN_9103_v109_revJ_13Jul2020EnglishGridion Download 27th April 2021 - Added screenshots of MinKNOW for barcoding recommendations in 'Data acquisition and basecalling' section
April 28th, 2021PTCN_9103_v109_revJ_13Jul2020EnglishMinion Download 27th April 2021 - Added screenshots of MinKNOW for barcoding recommendations in 'Data acquisition and basecalling' section
April 21st, 2021PTCN_9103_v109_revI_13Jul2020EnglishGridion Download 21st April 2021 - In the "Introduction to the protocol" section, "Overview of the protocol" step, added a list of products compatible with this protocol Inclusion of the optional Adapter Mix II Expansion (EXP-AMII001) kit for instances when a user is running a subset of barcodes in one experiment and needs extra Adapter Mix II for more reactions: - EXP-AMII001 is included in the "Equipment and consumables" section - Explanation of when to use EXP-AMII001 in the "Adapter ligation and clean-up" step
April 21st, 2021PTCN_9103_v109_revI_13Jul2020EnglishMinion Download 21st April 2021 - In the "Introduction to the protocol" section, "Overview of the protocol" step, added a list of products compatible with this protocol Inclusion of the optional Adapter Mix II Expansion (EXP-AMII001) kit for instances when a user is running a subset of barcodes in one experiment and needs extra Adapter Mix II for more reactions: - EXP-AMII001 is included in the "Equipment and consumables" section - Explanation of when to use EXP-AMII001 in the "Adapter ligation and clean-up" step
March 18th, 2021PTCN_9103_v109_revH_13Jul2020EnglishGridion Download 18th March 2021 - Updated 'Native barcode ligation' step to include instructions to take forward 30-50 ng of pooled barcoded samples in 30 µl into the 'Adapter ligation and clean-up step'. - Updated 'Adapter ligation and clean-up' step to include instructions to use 30-50 ng of pooled barcoded sample in the adapter ligation mastermix. - "Downstream analysis and expected results" step: the recommended analysis pipeline and workflow packaging information has been updated. We have removed the recommendation to use RAMPART and have included the EPI2ME Labs Jupyter notebook tutorial and EPI2ME workflow.
March 18th, 2021PTCN_9103_v109_revH_13Jul2020EnglishMinion Download 18th March 2021 - Updated 'Native barcode ligation' step to include instructions to take forward 30-50 ng of pooled barcoded samples in 30 µl into the 'Adapter ligation and clean-up step'. - Updated 'Adapter ligation and clean-up' step to include instructions to use 30-50 ng of pooled barcoded sample in the adapter ligation mastermix. - "Downstream analysis and expected results" step: the recommended analysis pipeline and workflow packaging information has been updated. We have removed the recommendation to use RAMPART and have included the EPI2ME Labs Jupyter notebook tutorial and EPI2ME workflow.
January 22nd, 2021PTCN_9103_v109_revG_13Jul2020EnglishGridion Download 11th Jan Updated mastermix volumes to volumes required per sample
January 22nd, 2021PTCN_9103_v109_revG_13Jul2020EnglishMinion Download 11th Jan Updated mastermix volumes to volumes required per sample
January 10th, 2021PTCN_9103_v109_revF_13Jul2020EnglishGridion Download 11th Dec Name updated from COVID-19 to SARS-CoV-2
January 10th, 2021PTCN_9103_v109_revF_13Jul2020EnglishMinion Download 11th Dec Name updated from COVID-19 to SARS-CoV-2
January 10th, 2021PTCN_9103_v109_revF_13Jul2020EnglishAny Download 11th Dec Name updated from COVID-19 to SARS-CoV-2
December 8th, 2020PTCN_9103_v109_revE_13Jul2020EnglishGridion Download 8th Dec Compatibilities updated to EXP-WSH004
December 8th, 2020PTCN_9103_v109_revE_13Jul2020EnglishMinion Download 8th Dec Compatibilities updated to EXP-WSH004
November 24th, 2020PTCN_9103_v109_revD_13Jul2020EnglishGridion Download 24th Nov Updated timings and added workflow image
November 24th, 2020PTCN_9103_v109_revD_13Jul2020EnglishMinion Download 24th Nov Updated timings and added workflow image
November 10th, 2020PTCN_9103_v109_revC_13Jul2020EnglishGridion Download
November 10th, 2020PTCN_9103_v109_revC_13Jul2020EnglishMinion Download
July 29th, 2020PTCN_9103_v109_revB_13Jul2020EnglishGridion Download 29th July 2020 Added SFB expansion to the equipment and consumables list.
July 29th, 2020PTCN_9103_v109_revB_13Jul2020EnglishMinion Download 29th July 2020 Added SFB expansion to the equipment and consumables list.
July 13th, 2020PTCN_9103_v109_revA_13Jul2020EnglishGridion Download
July 13th, 2020PTCN_9103_v109_revA_13Jul2020EnglishMinion Download
July 2nd, 2020PCB_9096_v109_revC_14May2020EnglishGridion Download 18th May Updated with corrections to RT step and Native Barcode Ligation
July 2nd, 2020PCB_9096_v109_revC_14May2020EnglishMinion Download 18th May Updated with corrections to RT step and Native Barcode Ligation
May 20th, 2020PCB_9096_v109_revB_14May2020English Download 18th May Updated with corrections to RT step and Native Barcode Ligation
May 14th, 2020PCB_9096_v109_revA_14May2020English Download 29th April 2020 Minor corrections to RNA dilution guidelines depending on qPCR Ct results