14th Sept 2021
- In "Overview of the protocol" and "Equipment and consumables", there is a note that the 96 barcode orientation on the plate differs depending on the kit batch number
14th Sept 2021
- In "Overview of the protocol" and "Equipment and consumables", there is a note that the 96 barcode orientation on the plate differs depending on the kit batch number
16th June 2021
- Required settings in MinKNOW in the 'Sequencing and data analysis' section has been updated include instructions to toggle 'Override mid barcoding score' and to increase minimum mid barcoding score from 40 to 50.
16th June 2021
- Required settings in MinKNOW in the 'Sequencing and data analysis' section has been updated include instructions to toggle 'Override mid barcoding score' and to increase minimum mid barcoding score from 40 to 50.
21st April 2021
- In the "Introduction to the protocol" section, "Overview of the protocol" step, added a list of products compatible with this protocol
Inclusion of the optional Adapter Mix II Expansion (EXP-AMII001) kit for instances when a user is running a subset of barcodes in one experiment and needs extra Adapter Mix II for more reactions:
- EXP-AMII001 is included in the "Equipment and consumables" section
- Explanation of when to use EXP-AMII001 in the "Adapter ligation and clean-up" step
21st April 2021
- In the "Introduction to the protocol" section, "Overview of the protocol" step, added a list of products compatible with this protocol
Inclusion of the optional Adapter Mix II Expansion (EXP-AMII001) kit for instances when a user is running a subset of barcodes in one experiment and needs extra Adapter Mix II for more reactions:
- EXP-AMII001 is included in the "Equipment and consumables" section
- Explanation of when to use EXP-AMII001 in the "Adapter ligation and clean-up" step
18th March 2021
- Updated 'Native barcode ligation' step to include instructions to take forward 30-50 ng of pooled barcoded samples in 30 µl into the 'Adapter ligation and clean-up step'.
- Updated 'Adapter ligation and clean-up' step to include instructions to use 30-50 ng of pooled barcoded sample in the adapter ligation mastermix.
- "Downstream analysis and expected results" step: the recommended analysis pipeline and workflow packaging information has been updated. We have removed the recommendation to use RAMPART and have included the EPI2ME Labs Jupyter notebook tutorial and EPI2ME workflow.
18th March 2021
- Updated 'Native barcode ligation' step to include instructions to take forward 30-50 ng of pooled barcoded samples in 30 µl into the 'Adapter ligation and clean-up step'.
- Updated 'Adapter ligation and clean-up' step to include instructions to use 30-50 ng of pooled barcoded sample in the adapter ligation mastermix.
- "Downstream analysis and expected results" step: the recommended analysis pipeline and workflow packaging information has been updated. We have removed the recommendation to use RAMPART and have included the EPI2ME Labs Jupyter notebook tutorial and EPI2ME workflow.