11 December 2024
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/11/2024
RAA_9198_v114_revK_11Dec2024
English
MinION
11 December 2024
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
11/15/2024
RAA_9198_v114_revJ_20Nov2024
English
GridION
November 2024
- Updated kit content information and image to include new kit format for SQK-RBK114.24 with plated rapid barcodes.
11/15/2024
RAA_9198_v114_revJ_20Nov2024
English
MinION
November 2024
- Updated kit content information and image to include new kit format for SQK-RBK114.24 with plated rapid barcodes.
9/30/2024
RAA_9198_v114_revI_30Sept2024
English
GridION
30th September 2024
- Moved protocol out of early access phase, removed early access wording.
9/30/2024
RAA_9198_v114_revI_30Sept2024
English
MinION
30th September 2024
- Moved protocol out of early access phase, removed early access wording.
4/29/2024
RAA_9198_v114_revH_29Nov2023
English
GridION
29 April 2024
- Updated references to EPI2ME (no longer called EPI2ME Labs) throughout document.
4/29/2024
RAA_9198_v114_revH_29Nov2023
English
MinION
29 April 2024
- Updated references to EPI2ME (no longer called EPI2ME Labs) throughout document.
02 April 2024
- Added note to 'Introduction to amplicon sequencing using the Rapid Barcoding Kit 24 or 96 V14' to specify correct primer design prior to amplicon generation.
- Added "Important" section to 'Equipment and Consumables' highlighting primer design and consideractions prior to amplcion generation.
02 April 2024
- Added note to 'Introduction to amplicon sequencing using the Rapid Barcoding Kit 24 or 96 V14' to specify correct primer design prior to amplicon generation.
- Added "Important" section to 'Equipment and Consumables' highlighting primer design and consideractions prior to amplcion generation.
January 2024
- Added wording throughout protocol to clarify each sample/barcode should be associated to a single species amplicon (this method is not suitable for mixed species amplicon samples).
January 2024
- Added wording throughout protocol to clarify each sample/barcode should be associated to a single species amplicon (this method is not suitable for mixed species amplicon samples).