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PromethION 2 Integrated logo imagePromethION 2 Integrated

PromethION 2 Integrated with flow cell lid open and screen active

Small box, big picture

All-in-one

Integrated, high-performance compute with touchscreen control.

Compact

Small footprint designed for any lab environment.

High-output

Ideal for multiomic applications, including human and large-genome sequencing, isoform-level transcriptomics, and metagenomics.


Overview

PromethION 2 Integrated is as powerful as it is streamlined. Designed to give more labs access to high-output sequencing, it’s able to run up to two independent PromethION Flow Cells, supported by high-performance onboard compute for real-time sequencing and analysis.

When you need a multiomic view to see the bigger picture, this is the fully integrated sequencer you can count on for greater insights into human health and disease.

PromethION 2 Integrated top angle with flow cell lid open

See performance specification

  • Compare performance across DNA and RNA applications - review output, read accuracy and modification detection.

  • Plan your lab setup with confidence - access key instrument specifications in one downloadable spec sheet.

PromethION 2 Specification sheet cover image

Specifications

A self-contained benchtop nanopore sequencer incorporating a powerful NVIDIA A-Series GPU and running two high-output PromethION Flow Cells.

Read length
Nanopore sequencing reads the entire length of DNA or RNA fragments, from short to ultra-long (20 bp to >4 Mb).

Dimensions
Size (W x H x D) | Weight:
25 mm x 180 mm x 430 mm | 10.6 kg
(8.9 in x 7.1 in x 16.9 in | 23.4 lb)

Two independent high-output flow cells
No more sample batching, just run.

Compact, fully integrated device
Powerful onboard compute for real-time insights.

High-resolution touchscreen
For complete device control

PromethION 2 Integrated image

Suitable applications

Flow cell specification

High-output flow cell, delivering any read length (20 bp to >4 Mb)

Multiplex up to 96 samples with PCR or PCR-free barcoding

flowcell 370

Ultra-deep cDNA sequencing for isoform-level transcript analysis and at single-cell resolution

High-output ultra-long native human DNA sequencing (read N50: >50 kb)

Comprehensive metagenomic characterisation (read N50: 10 kb)

EPI2ME Benchmarking data

PromethION 2 Data

Human variation

Pharmacogenomics (PGx)

Hereditary cancer

Microbial amplicon sequencing

PromethION Flow Cell output

~125 -160 Gb | ~10-25 M reads

(~40-50x human genome coverage)

~76 -82 Gb | ~135 M reads

(~233-254x total on-target coverage, ~36-47x per barcode coverage with 8 samples/device)

~70 Gb | ~115 M reads

(~208x total on-target coverage, ~69x per barcode coverage with 3 samples/flow cell)

~52 -61 Gb | ~34-39 M reads

File sizes

Reads: ~75-96 GB
Analysis: ~132 GB

Reads: ~46-50 GB
Analysis: ~11 GB

Reads: ~40 GB
Analysis: ~83 GB

Reads: ~31-37 GB
Analysis: ~13 MB

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Latest resources featuring PromethION 2 Integrated

Product documentation

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