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Plasmid sequencing from DNA using SQK-RBK114 (.24 or .96) (PRB_9188_v114_revI_09May2025)

DateVersionLanguageDeviceChanges
8/8/2025PRB_9188_v114_revI_09May2025EnglishGridION
09 May 2025 - Protocol title update
8/8/2025PRB_9188_v114_revI_09May2025EnglishMinION
09 May 2025 - Protocol title update
12/11/2024PRB_9188_v114_revH_11Dec2024EnglishGridION
11 December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Updated link for EPI2ME. - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/11/2024PRB_9188_v114_revH_11Dec2024EnglishMinION
11 December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Updated link for EPI2ME. - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
11/15/2024PRB_9188_v114_revG_20Nov2024EnglishGridION
November 2024 - Updated kit content information and image to include new kit format for SQK-RBK114.24 with plated rapid barcodes.
11/15/2024PRB_9188_v114_revG_20Nov2024EnglishMinION
November 2024 - Updated kit content information and image to include new kit format for SQK-RBK114.24 with plated rapid barcodes.
9/30/2024PRB_9188_v114_revF_30Sept2024EnglishGridION
30th September 2024 - Moved protocol out of early access phase, removed early access wording.
9/30/2024PRB_9188_v114_revF_30Sept2024EnglishMinION
30th September 2024 - Moved protocol out of early access phase, removed early access wording.
6/25/2024PRB_9188_v114_revE_17May2023EnglishGridION
14th November 2023 - Updated GridION branch to include troubleshooting section
6/25/2024PRB_9188_v114_revE_17May2023EnglishMinION
14th November 2023 - Updated GridION branch to include troubleshooting section
November 14th, 2023PRB_9188_v114_revC_17May2023EnglishGridion Download 3rd October 2023 - Checklist removed due to formatting issues
November 14th, 2023PRB_9188_v114_revC_17May2023EnglishMinion Download 3rd October 2023 - Checklist removed due to formatting issues
October 3rd, 2023PRB_9188_v114_revB_17May2023EnglishGridion Download 19th September 2023 - Updated flow cell loading instructions to include light shield installation
October 3rd, 2023PRB_9188_v114_revB_17May2023EnglishMinion Download 19th September 2023 - Updated flow cell loading instructions to include light shield installation
September 19th, 2023PRB_9188_v114_revA_17May2023EnglishGridion Download -
September 19th, 2023PRB_9188_v114_revA_17May2023EnglishMinion Download -

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