cDNA-PCR Sequencing (SQK-PCS111)

DateVersion numberLanguageDeviceChanges
Dec 6 2023PCS_9143_v111_revQ_01Dec2021EnglishGridION
December 2023 - Updated product phase to 'Legacy'
Dec 6 2023PCS_9143_v111_revQ_01Dec2021EnglishMinION
December 2023 - Updated product phase to 'Legacy'
Dec 6 2023PCS_9143_v111_revQ_01Dec2021EnglishPromethION
December 2023 - Updated product phase to 'Legacy'
September 19th, 2023PCS_9143_v111_revO_01Dec2021EnglishMinion Download March 2023 - Updated sequencing and data analysis to be aligned with our standard recommendations
September 19th, 2023PCS_9143_v111_revO_01Dec2021EnglishGridion Download March 2023 - Updated sequencing and data analysis to be aligned with our standard recommendations
September 19th, 2023PCS_9143_v111_revO_01Dec2021EnglishPromethion Download March 2023 - Updated sequencing and data analysis to be aligned with our standard recommendations
February 8th, 2023PCS_9143_v111_revN_01Dec2021EnglishMinion Download February 2023 - Removed the checklist download to fix formatting issues.
February 8th, 2023PCS_9143_v111_revN_01Dec2021EnglishGridion Download February 2023 - Removed the checklist download to fix formatting issues.
February 8th, 2023PCS_9143_v111_revN_01Dec2021EnglishPromethion Download February 2023 - Removed the checklist download to fix formatting issues.
January 6th, 2023PCS_9143_v111_revM_01Dec2021EnglishMinion Download January 2023 - Changed the PromethION "Priming and loading the flow cell" section to the updated version.
January 6th, 2023PCS_9143_v111_revM_01Dec2021EnglishGridion Download January 2023 - Changed the PromethION "Priming and loading the flow cell" section to the updated version.
January 6th, 2023PCS_9143_v111_revM_01Dec2021EnglishPromethion Download January 2023 - Changed the PromethION "Priming and loading the flow cell" section to the updated version.
December 7th, 2022PCS_9143_v111_revK_01Dec2021EnglishMinion Download December 2022 - Included instruction for using RNA Control Sample to run a control experiment - Added tips throughout the protocol explaining the function of the reagents through the workflow
December 7th, 2022PCS_9143_v111_revK_01Dec2021EnglishGridion Download December 2022 - Included instruction for using RNA Control Sample to run a control experiment - Added tips throughout the protocol explaining the function of the reagents through the workflow
December 7th, 2022PCS_9143_v111_revK_01Dec2021EnglishPromethion Download December 2022 - Included instruction for using RNA Control Sample to run a control experiment - Added tips throughout the protocol explaining the function of the reagents through the workflow
August 10th, 2022PCS_9143_v111_revI_01Dec2021EnglishMinion Download 20th June 2022 - Update the priming and loading the SpotON flow cells for both MinION and GridION with new diagrams.
August 10th, 2022PCS_9143_v111_revI_01Dec2021EnglishGridion Download 20th June 2022 - Update the priming and loading the SpotON flow cells for both MinION and GridION with new diagrams.
August 10th, 2022PCS_9143_v111_revI_01Dec2021EnglishPromethion Download 20th June 2022 - Update the priming and loading the SpotON flow cells for both MinION and GridION with new diagrams.
May 25th, 2022PCS_9143_v111_revH_01Dec2021EnglishMinion Download 25th May 2022 - Protocol overview workflow image updated
May 25th, 2022PCS_9143_v111_revH_01Dec2021EnglishGridion Download 25th May 2022 - Protocol overview workflow image updated
May 25th, 2022PCS_9143_v111_revH_01Dec2021EnglishPromethion Download 25th May 2022 - Protocol overview workflow image updated
April 13th, 2022PCS_9143_v111_revG_01Dec2021EnglishMinion Download 14th April 2022 Protocol name change
April 13th, 2022PCS_9143_v111_revG_01Dec2021EnglishGridion Download 14th April 2022 Protocol name change
April 13th, 2022PCS_9143_v111_revG_01Dec2021EnglishPromethion Download 14th April 2022 Protocol name change
January 11th, 2022PCS_9143_v111_revF_01Dec2021EnglishMinion Download 11th January 2022 - Updated the Priming and loading the spotON flow cell for GridION branch
January 11th, 2022PCS_9143_v111_revF_01Dec2021EnglishGridion Download 11th January 2022 - Updated the Priming and loading the spotON flow cell for GridION branch
January 11th, 2022PCS_9143_v111_revF_01Dec2021EnglishPromethion Download 11th January 2022 - Updated the Priming and loading the spotON flow cell for GridION branch
January 7th, 2022PCS_9143_v111_revE_01Dec2021EnglishMinion Download 06th January 2022 - Added new branches to the protocol for MinION and GridION devices. - Updated the library prep timings
January 7th, 2022PCS_9143_v111_revE_01Dec2021EnglishGridion Download 06th January 2022 - Added new branches to the protocol for MinION and GridION devices. - Updated the library prep timings
January 7th, 2022PCS_9143_v111_revE_01Dec2021EnglishPromethion Download 06th January 2022 - Added new branches to the protocol for MinION and GridION devices. - Updated the library prep timings
December 10th, 2021PCS_9143_v111_revD_01Dec2021English Download 9th December 2021 - Added reference to third party reagent preparation and NEBNext Buffer mixing - Changed the "Data acquisition and basecalling" section to focus on PromethION - In "Priming and loading the flow cell" have moved up the recomendation of letting flow cells reach room temperature after taking out of the fridge to before loading the flow cells 2nd December 2021 - Updated to focus the protocol on PromethION device - Protocol workflow image has been updated to with a new font - Removed the kit contents image to be updated in the near future - Added reagent preparation snippets - Clarified mastermix is added to the PCR tubes with the RT RNA samples in "Selecting for full-length transcripts by PCR" - Removed references to R10.4 - Added Elution Buffer (EB) to the materials list in "Adapter addition"
December 10th, 2021PCS_9143_v111_revC_02Dec2021English Download 9th December 2021 - Added the compatibility with the MinION R9.4.1 flow cells (FLO-MIN106D and FLO-MINSP6) - Added reference to third party reagent preparation and NEBNext Buffer mixing 2nd December 2021 - Updated to focus the protocol on PromethION device - Protocol workflow image has been updated to with a new font - Removed the kit contents image to be updated in the near future - Added reagent preparation snippets - Clarified mastermix is added to the PCR tubes with the RT RNA samples in "Selecting for full-length transcripts by PCR" - Removed references to R10.4 - Added Elution Buffer (EB) to the materials list in "Adapter addition"
December 2nd, 2021PCS_9143_v111_revB_01Dec2021English Download 2nd December 2021 - Updated to focus the protocol on PromethION device - Protocol workflow image has been updated to with a new font - Removed the kit contents image to be updated in the near future - Added reagent preparation snippets - Clarified mastermix is added to the PCR tubes with the RT RNA samples in "Selecting for full-length transcripts by PCR" - Removed references to R10.4 - Added Elution Buffer (EB) to the materials list in "Adapter addition"
December 1st, 2021PCS_9143_v111_revA_01Dec2021EnglishPromethion Download
December 1st, 2021PCS_9143_v111_revA_01Dec2021EnglishGridion Download
December 1st, 2021PCS_9143_v111_revA_01Dec2021EnglishMinion Download
November 25th, 2020PCR-cDNA_PCS110_v3EnglishPromethion Download
November 25th, 2020PCR-cDNA_PCS110_v3EnglishGridion Download
November 25th, 2020PCR-cDNA_PCS110_v3EnglishMinion Download
November 25th, 2020PCR-cDNA_PCS110_v3EnglishAny Download
October 9th, 2020PCR-cDNA_PCS110_v2EnglishPromethion Download
October 9th, 2020PCR-cDNA_PCS110_v2EnglishGridion Download
October 9th, 2020PCR-cDNA_PCS110_v2EnglishMinion Download
October 8th, 2020PCR-cDNA_PCS110_v1English Download