Taking your single-cell sequencing to new lengths with Oxford Nanopore
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The analysis of genomic heterogeneity at the single-cell level has provided new insights into many research areas, including cancer research, cell development and function, and immunology. However, the use of short-read sequencing limits the ability to identify isoforms, alternative splicing, variants, and other biologically important features. Nanopore sequencing resolves this challenge by enabling the analysis of full-length transcripts to gain a deeper understanding of complex biology.
Join this Knowledge Exchange to learn more about implementing the Oxford Nanopore single-cell sequencing workflow — from library preparation to data analysis — with our in-house experts. The presentations will be followed by a live Q&A session.
You will hear about:
Best practices for library preparation with 10x Genomics full-length cDNA
Easy-to-implement data analysis with our EPI2ME workflow: wf-single-cell
Example data analysis outputs, including UMAPs and tertiary analysis compatible files
Please note, this Knowledge Exchange will broadcast at 7am (GMT)/3pm (SGT) and 4pm (GMT)/12pm (EDT). You can select the one suitable for you below.
Meet the speakers
A short overview of the wet lab protocol, from 10x cDNA sample through to nanopore sequencing: 1. Benefits of nanopore sequencing full length single cell cDNA. 2. Current 10x genomic kit compatibility. 3. 10x chromium 3' expression protocol overview. 4. Example performance.
A short overview of the wet lab protocol, from 10x cDNA sample through to nanopore sequencing: 1. Benefits of nanopore sequencing full length single cell cDNA. 2. Current 10x genomic kit compatibility. 3. 10x chromium 3' expression protocol overview. 4. Example performance.
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Rebecca Pawluk, Development Scientist in Sample Technology, Oxford Nanopore- An overview of the data analysis workflow (wf-single-cell).
- How to install and run wf-single cell from within EPI2ME desktop app and at the commend line.
- What outputs should users expect when running the workflow.
- An overview of the data analysis workflow (wf-single-cell).
- How to install and run wf-single cell from within EPI2ME desktop app and at the commend line.
- What outputs should users expect when running the workflow.
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Neil Horner, Software Developer, Oxford Nanopore)
Shauna Clark, Segment Marketing Manager, Single Cell, Oxford NanoporeShauna Clark is the Segment Marketing Manager, Single Cell at Oxford Nanopore
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Ann Chia, Inside Sales Specialist, Oxford NanoporeAnn is currently serving as an Oxford Nanopore Account Specialist within the APAC team. She holds a master’s degree in Crop Biotechnology from the University of Nottingham. Before joining Oxford Nanopore Technologies, Ann served as a Regional Sales Manager overseeing the NGS consumables market in Southeast Asia and Northeast Asia. With over eight years of experience in sequencing, she has transitioned through roles involving Sanger sequencing, being a short-read sequencing provider, NGS consumables, and now specialises in nanopore technology.
