Guppy
Guppy is a data processing toolkit that contains the Oxford Nanopore Technologies' production basecalling algorithms and several bioinformatic post-processing features.
Note:
This software is no longer supported, and we recommend all customers to upgrade to the latest basecaller, Dorado.
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Version: 6.5.7
Release date: 11/15/2023
Approved use: For research use only
Features:
- Added m6A DRACH-context modified base model for use with RNA004
- Added poly(A)/poly(T) tail length estimation for direct RNA and cDNA
- Added RNA read splitting to separate concatenated reads and enabled RNA adapter trimming
- Reductions to memory usage when performing duplex basecalling
- Bug fixes, including addressing auto batch size issues and demux crashes
Nanopore Community
The Nanopore Community contains many useful resources, including:
Protocols
Build or select a protocol using our range of tools.
Bioinformatics
Build or select a protocol using our range of tools.
Extraction methods
Detailed extraction protocols and specific recommendations are provided for various tissue types.