30th April 2024
- Corrected the specifications in "Device information"
- "What's in the box" has been updated to include 1x MiniDP/Display port cable, 1x USB-A stick (ISO reset image), and 1x USB-C/USB-A adapter
- Added information about the PromethION 24 Combined in "Device information" and "What's in the box", with a disclaimer: "The PromethION 24 Combined must only be unpacked and installed by an Oxford Nanopore Technologies Field Service Engineer."
9th August 2023
- The device name has been updated from A100 to A-Series
- The wording throughout the protocol has been updated for simplicity and clarity
13th March 2023
- In "Operating system update" - "If problems are encountered", a note has been added to say "During the process to blank all of the system’s disks, the following screen will be visible showing either ASRock or ASUS with a scrolling circle. This process may take time to complete and be following by a reboot."
10th March 2023
- In "Operating system update" - "If problems are encountered", a new FAQ was added about performing a secure wipe.
- "Downloading the ISO image and burning it to a USB drive" - the link to the ISO image download has been updated. A comment has been added to the Overview to say "These instructions are for users who did not receive a USB drive with the ISO image. If you have received a USB drive, proceed to the next step: "How to boot from USB"."
February 2023
- Updated information in "Installing and powering on the device" for how users log into MinKNOW software using their Community credentials which now requires the use of OKTA as user authentification.
15th September 2022
- "Device information" - added link to the PromethION P24/P48 A100 technical specification document.
- "Downloading the ISO image and burning it to a USB drive": the download link and MD5 hash have been updated. The USB drive specification has been changed from 16 GB to 32 GB.
- "How to boot from USB": a note has been added that once the rebuild process has been completed and the device has had a power cycle, any previous changes to the device password will have defaulted back to the default password.
24th August 2022
Updated to reflect main updates for MinKNOW 22.08 release:
- 'Updating the software' includes information about update availability and rebooting
Other updates:
- 'Flow cell check': the instruction for starting the flow cell check has been updated to say "When you see the flow cell type and flow cell IDs recognised, click 'Start' to begin."
5th May 2022
"Overview" - the technical specification has been updated to include the new PromethION A100 devices.
"Installing and powering on the device" - instructions have been updated to include the new PromethION A100 devices.
"Data management" - several instruction steps have been updated to include Focal Ubuntu instructions, update installation commands, include steps on how to start/stop the data transfer, and fix issues with code blocks.
In "Downloading the ISO image and burning it to a USB drive":
- the Rufus link has been corrected to https://rufus.ie/en/
- the ISO image download link has been updated to include file hash information
In "Completing the restore", a new instruction has been added: "Eject the bootable USB by going to the File Explorer and selecting the Eject icon next to the USB device."
21st July 2021
The document has been renamed to PromethION user manual, with the specific changes below:
- The structure of the document has changed to include the sections "Overview", "Installation", "Pre-sequencing checks and sequencing run", "After completing a sequencing run", "Updating the software" and "Troubleshooting"
- Hardware check and flow cell check instructions added
- New instructions added for data management from the command-line
16th June 2021
- Updated all screenshots of the MinKNOW GUI to the new 4.3 release
- Updated screenshot for loggin on to MinKNOW and sequencing overview on 'Logging on' in the 'Installation' section
- Added screenshot of the start page and Hardware check button highlighted and updated the hardware check page in the 'Installation: Running the hardware check' section
9th July 2020
New UI across all devices
Alignment running in MinKNOW
Start of security upgrade - Login
Custom filtering of reads - q-score and read lengths
New barcoding options in the UI
Guppy 4.0 including new basecall models
Admin interface
Update to use Python 3.7
Various improvements to the scripts - new selection criteria at MUX scan, better handling of low-range reads and global flick and drift improvements
ont-vbz-hdf-plugin added as part of the release
Support for upcoming kits and expansion packs - Ligation Sequencing Kit (SQK-LSK110), Native Barcoding Expansion 1-96 (EXP-NBD196) and Rapid Barcoding Kit SQK-RBK096