Main menu

Rabbit blood DNA – QIAGEN Genomic-tip 500/G


Materials

  • 10 ml of blood, collected in K2-EDTA
  • QIAGEN Blood and Cell Culture DNA Maxi kit
  • 70% ethanol in nuclease-free water
  • Isopropanol
  • TE buffer (1 mM EDTA, pH 8.0)
  • 1.5 ml Eppendorf DNA LoBind tubes
  • 50 ml Falcon tubes
  • Centrifuge and rotor for 50 ml Falcon tubes
  • Incubator or water bath set at 50°C

Method

  1. Perform cell separation and lysis according to the QIAGEN protocol (Preparation of blood samples section) (page 21).
  2. Critical step: Lyse the nuclei for 1 hour at 50°C to ensure the lysis of all the cells, using the reagent quantities described in the protocol (page 24).
  3. Purify the lysate according to the QIAGEN protocol (Isolation of Genomic DNA from Blood, Cultured Cells, Tissue, Yeast, or Bacteria using Genomic-tips section) (page 49).
  4. Critical step: To maximize the DNA yield we recommend that the elution is performed for 2 hours at 50°C, using 200 µl TE (1 mM EDTA, pH 8.0), occasionally mixing the tube contents by gentle inversion.
  5. Take 3 µg of eluate and perform a SPRI size selection.

Results

  • Yield: 90-120 μg
  • OD 260/280: 1.93
  • OD 260/230: 2.33

gTip nanodrop

Sequencing performance

Libraries were prepared using the Ligation Sequencing Kit.

  • Read length profile:

Rabbit blood G-tip

Change log

Version Change
v1, 27th July 2019 Initial protocol publication
v2, 14th August 2023 Updated protocol links and page numbers

Last updated: 8/14/2023

文档选项

语言:

入门指南

购买 MinION 启动包 Nanopore 商城 测序服务提供商 全球代理商

纳米孔技术

订阅 Nanopore 更新 资源库及发表刊物 什么是 Nanopore 社区

关于 Oxford Nanopore

新闻 公司历程 可持续发展 领导团队 媒体资源和联系方式 投资者 合作者 在 Oxford Nanopore 工作 职位空缺 商业信息 BSI 27001 accreditationBSI 90001 accreditationBSI mark of trust
Chinese flag