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Software release 24.02.16 for MinKNOW for MinION Mk1C

Version: 24.02.16

Release date: 5/1/2024

Features

  • Complete redesign of the MinKNOW file output options interface and experience
  • New v4.3 basecalling models
  • New Q-score histogram data visualisation for Simplex data
  • Q-accuracy graph when alignment is used
  • MinKNOW is now available in Spanish! ¡MinKNOW ya está disponible en español!

Improvements

  • Updated MinKNOW tooltips after a program-wide review
  • Run report improvements including more accurate display of unclassified data, displaying more user-selected settings, and more
  • Improved error messages around availability and status of the MinKNOW basecalling service
  • Low disk space warnings at run setup
  • Improved design of the basecalling/barcoding/alignment settings page
  • Improvements to RNA trimming strategy and updates to Q score prediction
  • Barcode alias from sample sheet now included in both directory name and file name
  • Run report updates:
  • Q-score histogram included in run report
  • Number of passed and failed reads shown on the run report
  • Report adaptive sampling bed file detail
  • Information regarding the quantity of unclassified barcodes
  • Read splitting defaulted to ON for all conditions. Options to change this have been removed as part of UI simplification.
  • Script Updates: the SQK-ULK114 kit to allows more time for reads to finish before a pore scan and changes to adaptive sampling that should improve rejection slightly

Bug Fixes

  • Adaptive Sampling no longer pre-populates alignment references
  • Removed official support for mmi in MinKNOW
  • Fixes to prevent reads being incorrectly split in MinKNOW
  • Search barcodes on run report now functions as expected
  • Flow Cell check on a FLO-MIN114 flow cell after using the wash kit provides more accurate pore counts
  • P2 Solo firmware update providing stability improvements
  • Fix for stability issues reported by some customers
  • Additional fields in the sample sheet will not be replicated on the copy output by MinKNOW
  • Updates to UI elements showing misleading information
  • Run report target temperature not changing to reflect the set temperature
  • Raw file output for SQK-RNA004 defaults to POD5 rather than FAST5 output
  • Fixed overlapping text on experiment page table

Last updated: 8/6/2025

Latest version: 25.05.14

Operating system: Linux

Approved use: For research use only

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