Ligation sequencing V14 — single-cell transcriptomics with 5' cDNA prepared using 10X Genomics on PromethION (SQK-LSK114) (SST_9204_v114_revJ_25Feb2025)

DateVersionLanguageDeviceChanges
4/9/2025SST_9204_v114_revJ_25Feb2025EnglishPromethION
25th February 2025 In "Overview of the protocol" and "Equipment and consumables", compatibility information has been corrected to say "This protocol is intended for use with the 10X Genomics GEM-X Single Cell 5' Kit (V3) or the Next GEM Single Cell 5' Kit (V2)."
1/29/2025SST_9204_v114_revH_29Jan2025EnglishPromethION
29th January 2025 In "PCR amplification", the quantification step has been changed to say "Quantify 1 µl of eluted sample using a Qubit fluorometer - recovery aim >100 ng total.".
11/6/2024SST_9204_v114_revG_06Mar2024EnglishPromethION
November 2024 - Formatting fix to custom-ordered oligo sequences table in equipment and consumables to prevent some of the sequence being cut off in the PDF generation.
8/21/2024SST_9204_v114_revF_06Mar2024EnglishPromethION
21st August 2024 - NEB reagent recommendations for purchase and use updated (Salt T4 ligase in place of Quick Ligation Module). - Updated the Adapter ligation step with new recommended method.
August 21st, 2024SST_9204_v114_revE_31Jul2024English- Download 31 July 2024 - Changed reagent order addition in Library preparation/Adapter ligation and clean-up; Ligation Adapter (LA) added before Ligation Buffer (LB) and NEBNext Quick T4 Ligase 07 May 2024 - Updated library loading recommendation at the end of "Adapter ligation and clean-up" to use 50–100 fmol going into the flow cell.
May 14th, 2024SST_v9204_v114_revD_06Mar2024English- Download 07 May 2024 - Updated library loading recommendation at the end of "Adapter ligation and clean-up" to use 50–100 fmol going into the flow cell.
April 12th, 2024SST_v9204_v114_revA_06Mar2024English- Download -