Ligation sequencing V14 — single-cell transcriptomics with 5' cDNA prepared using 10X Genomics on PromethION (SQK-LSK114) (SST_9204_v114_revH_29Jan2025)


DateVersionLanguageDeviceChanges
1/29/2025SST_9204_v114_revH_29Jan2025EnglishPromethION
29th January 2025 In "PCR amplification", the quantification step has been changed to say "Quantify 1 µl of eluted sample using a Qubit fluorometer - recovery aim >100 ng total.".
11/6/2024SST_9204_v114_revG_06Mar2024EnglishPromethION
November 2024 - Formatting fix to custom-ordered oligo sequences table in equipment and consumables to prevent some of the sequence being cut off in the PDF generation.
8/21/2024SST_9204_v114_revF_06Mar2024EnglishPromethION
21st August 2024 - NEB reagent recommendations for purchase and use updated (Salt T4 ligase in place of Quick Ligation Module). - Updated the Adapter ligation step with new recommended method.
August 21st, 2024SST_9204_v114_revE_31Jul2024English- Download 31 July 2024 - Changed reagent order addition in Library preparation/Adapter ligation and clean-up; Ligation Adapter (LA) added before Ligation Buffer (LB) and NEBNext Quick T4 Ligase 07 May 2024 - Updated library loading recommendation at the end of "Adapter ligation and clean-up" to use 50–100 fmol going into the flow cell.
May 14th, 2024SST_v9204_v114_revD_06Mar2024English- Download 07 May 2024 - Updated library loading recommendation at the end of "Adapter ligation and clean-up" to use 50–100 fmol going into the flow cell.
April 12th, 2024SST_v9204_v114_revA_06Mar2024English- Download -