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Direct RNA sequencing (SQK-RNA004-XL) (DRS_9219_v4_revD_29Aug2025)

DateVersionLanguageDeviceChanges
8/29/2025DRS_9219_v4_revD_29Aug2025EnglishGridION
29th August 2025 - In 'Compatibility of this protocol', under the PromethION branch, the PromethION 2 Integrated was included as a compatible device and the link to its user manual was added to 'Data acquisition and basecalling'.
8/29/2025DRS_9219_v4_revD_29Aug2025EnglishMinION
29th August 2025 - In 'Compatibility of this protocol', under the PromethION branch, the PromethION 2 Integrated was included as a compatible device and the link to its user manual was added to 'Data acquisition and basecalling'.
8/29/2025DRS_9219_v4_revD_29Aug2025EnglishPromethION
29th August 2025 - In 'Compatibility of this protocol', under the PromethION branch, the PromethION 2 Integrated was included as a compatible device and the link to its user manual was added to 'Data acquisition and basecalling'.
7/16/2025DRS_9219_v4_revC_16Jul2025EnglishGridION
16th July 2025 - In 'Data acquisition and Basecalling', 'To start a sequencing run on MinKNOW', the script for the standard RNA004 was replaced with the script for RNA004-XL in step 3, as a result of the update to MinKNOW.
7/16/2025DRS_9219_v4_revC_16Jul2025EnglishMinION
16th July 2025 - In 'Data acquisition and Basecalling', 'To start a sequencing run on MinKNOW', the script for the standard RNA004 was replaced with the script for RNA004-XL in step 3, as a result of the update to MinKNOW.
7/16/2025DRS_9219_v4_revC_16Jul2025EnglishPromethION
16th July 2025 - In 'Data acquisition and Basecalling', 'To start a sequencing run on MinKNOW', the script for the standard RNA004 was replaced with the script for RNA004-XL in step 3, as a result of the update to MinKNOW.
5/19/2025DRS_9219_v4_revB_19May2025EnglishGridION
19th May 2025 - In 'Overview', library preparation time was increased to ~140 min to align with Table and Figure in 'Steps in the sequencing workflow' where priming and loading of flow cell was increased from 5 min to 10 min. - In section 4, 'Priming and loading the MinION/GridION Flow Cell'', an important note was added to the bottom of the section stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device.
5/19/2025DRS_9219_v4_revB_19May2025EnglishMinION
19th May 2025 - In 'Overview', library preparation time was increased to ~140 min to align with Table and Figure in 'Steps in the sequencing workflow' where priming and loading of flow cell was increased from 5 min to 10 min. - In section 4, 'Priming and loading the MinION/GridION Flow Cell'', an important note was added to the bottom of the section stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device.
5/19/2025DRS_9219_v4_revB_19May2025EnglishPromethION
19th May 2025 - In 'Overview', library preparation time was increased to ~140 min to align with Table and Figure in 'Steps in the sequencing workflow' where priming and loading of flow cell was increased from 5 min to 10 min. - In section 4, 'Priming and loading the MinION/GridION Flow Cell'', an important note was added to the bottom of the section stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device.
5/19/2025DRS_9219_v4_revB_19May2025EnglishGridION
19th May 2025 - In 'Overview', library preparation time was increased to ~140 min to align with Table and Figure in 'Steps in the sequencing workflow' where priming and loading of flow cell was increased from 5 min to 10 min. - In section 4, 'Priming and loading the MinION/GridION Flow Cell'', an important note was added to the bottom of the section stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device.
5/19/2025DRS_9219_v4_revB_19May2025EnglishMinION
19th May 2025 - In 'Overview', library preparation time was increased to ~140 min to align with Table and Figure in 'Steps in the sequencing workflow' where priming and loading of flow cell was increased from 5 min to 10 min. - In section 4, 'Priming and loading the MinION/GridION Flow Cell'', an important note was added to the bottom of the section stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device.
5/19/2025DRS_9219_v4_revB_19May2025EnglishPromethION
19th May 2025 - In 'Overview', library preparation time was increased to ~140 min to align with Table and Figure in 'Steps in the sequencing workflow' where priming and loading of flow cell was increased from 5 min to 10 min. - In section 4, 'Priming and loading the MinION/GridION Flow Cell'', an important note was added to the bottom of the section stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device.
2/19/2025DRS_9219_v4_revA_19Feb2025EnglishGridION
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2/19/2025DRS_9219_v4_revA_19Feb2025EnglishMinION
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2/19/2025DRS_9219_v4_revA_19Feb2025EnglishPromethION
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