NCM 2022: Amplification and sequencing of entire tick mitochondrial genomes for a phylogenomic analysis
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Many tick species are important vectors of disease causing pathogens Tick bites can also cause non-infectious syndromes such as alpha-gal syndrome (red meat allergy) and tick toxicosis Identifying ticks to the species-level is critical for surveillance efforts and tracking geographic distributions Mitochondrial genome (mitogenome) sequencing has been crucial for tick species identification, taxonomy, systematics, and surveillance Current strategies for tick mitogenome sequencing include low-coverage genome skimming and multiamplicon amplification and sequencing, both costly to scale Our strategy uses 2 full-length mitogenome PCRs with Oxford Nanopore Technologies Mk1B MinION platform for cost-effective sequencing at scale.