Enhancing nanopore adaptive sampling for PromethION using readfish at scale
Adaptive sampling enables live targeted Oxford Nanopore sequencing without additional library preparation. Here, Munro et al. have improved readfish — a tool that supports live adaptive sampling — so that it can handle the high data output of a PromethION device. Combining adaptive sampling with high-throughput sequencing increases coverage over targeted sequences, enhances SV and copy number detection, and reduces costs, making Oxford Nanopore sequencing the ideal solution for large-scale genomics and personalised medicine in the future.
Key points:
Readfish is now fully compatible with PromethION, offering both standard and barcode-aware adaptive sampling
The introduction of mappy-rs (a multithread alignment tool) eliminates computational bottlenecks, keeping up with the high data output of the PromethION device
Improved multiplexing efficiency allows researchers to sequence multiple human genomes on a single flow cell
'The application of adaptive sampling to nanopore sequencing can address longstanding challenges inherent to other sequencing methods. It can lower sequencing costs and save time, alongside improving the depth and quality of sequencing data for targeted genomic regions'
Sample type: simulated sequencing runs
Kit: Native Barcoding Kit