Main menu

Direct RNA sequencing of dsRNA using Oxford Nanopore Technologies


Double-stranded RNA (dsRNA) occurs in several biological phenomena, such as dsRNA viruses, intermediates of replicating single-stranded RNA viruses, and long cellular dsRNA. In recent decades, there has been increased interest in utilizing dsRNA for RNA interference, particularly for pest control in agricultural settings, necessitating the large-scale production of dsRNA. Novel methods for dsRNA characterization are therefore of interest for RNA manufacturing as well as for the study of RNA biology.

In this work, we describe the development of a protocol for direct RNA sequencing (DRS) of dsRNA using Oxford Nanopore Technologies.

Download the PDF

Getting started

Buy a MinION starter pack Nanopore store Sequencing service providers Channel partners

Nanopore technology

Subscribe to Nanopore updates Resources and publications What is the Nanopore Community

About Oxford Nanopore

News Company timeline Sustainability Leadership team Media resources & contacts For investors For partners Working at Oxford Nanopore Current vacancies Commercial information BSI 27001 accreditationBSI 90001 accreditationBSI mark of trust
Spanish flag