Direct RNA sequencing of dsRNA using Oxford Nanopore Technologies
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Double-stranded RNA (dsRNA) occurs in several biological phenomena, such as dsRNA viruses, intermediates of replicating single-stranded RNA viruses, and long cellular dsRNA. In recent decades, there has been increased interest in utilizing dsRNA for RNA interference, particularly for pest control in agricultural settings, necessitating the large-scale production of dsRNA. Novel methods for dsRNA characterization are therefore of interest for RNA manufacturing as well as for the study of RNA biology.
In this work, we describe the development of a protocol for direct RNA sequencing (DRS) of dsRNA using Oxford Nanopore Technologies.