10th August 2022
- The document has been renamed to "Restriction enzyme Pore-C info sheet"
- In "Library preparation", compatibility with the SQK-LSK114 Ligation Sequencing Kit has been added
- In "References", a new Pore-C publication has beed added: " Identifying synergistic high-order 3D chromatin conformations from genome-scale nanopore concatemer sequencing."
15th February 2022
- In "Library preparation", the recommendations have been updated to include the Ligation Sequencing Kits SQK-LSK110 and SQK-LSK112. The "Maximising output and accuracy" section has been expanded to explain which sequencing chemistry to use depending on the application.
- Links to the Extraction Methods and DNA/RNA handling sections of the Community have been updated.
- In "Application updates", a new application has been included: Pore-C scaffolding of genomic assemblies: a case study of multiple sample types
21st April 2021
- Updated 'Choosing a restriction enzyme' in 'Protocol considerations' with updated information regarding how to choose the most appropriate restriction enzyme.
- Updated 'Size selection' with an altered SPRI size selection to reduce the frequency of non-chimeric reads (singletons).
- Added 'Application updates' section to include links to the App update notes.
21st April 2021
- Updated 'Choosing a restriction enzyme' in 'Protocol considerations' with updated information regarding how to choose the most appropriate restriction enzyme.
- Updated 'Size selection' with an altered SPRI size selection to reduce the frequency of non-chimeric reads (singletons).
28th January 2021
- In the "Library preparation" section, "Library preparation recommendations" step, updated the Flow Cell Wash Kit recommendation and link to protocol from EXP-WSH003 to EXP-WSH004
28th January 2021
- In the "Library preparation" section, "Library preparation recommendations" step, updated the Flow Cell Wash Kit recommendation and link to protocol from EXP-WSH003 to EXP-WSH004