Ligation sequencing gDNA - low input by PCR (SQK-LSK110 with EXP-PCA001) (LWP_9117_v110_revK_10Nov2020)

DateVersionLanguageDeviceChanges
3/10/2023LWP_9117_v110_revK_10Nov2020EnglishGridION
February 2023 - Updated product phase to 'Legacy'
3/10/2023LWP_9117_v110_revK_10Nov2020EnglishMinION
February 2023 - Updated product phase to 'Legacy'
August 10th, 2022LWP_9117_v110_revJ_10Nov2020EnglishGridion Download 14th July 2022 - Updated compatibilities and removed references to discontinued flow cells
August 10th, 2022LWP_9117_v110_revJ_10Nov2020EnglishMinion Download 14th July 2022 - Updated compatibilities and removed references to discontinued flow cells
July 6th, 2022LWP_9117_v110_revI_10Nov2020EnglishGridion Download 06th July 2022 - Updated the snippet: "Prepare the NEBNext FFPE DNA Repair Mix and NEBNext Ultra II End repair / dA-tailing Module reagents in accordance with manufacturer’s instructions, and place on ice." to also include the manufacturers recommendations. - Removed reference to Kit 12 chemistry in the "Priming and loading the SpotON flow cell for GridION" section of the protocol.
July 6th, 2022LWP_9117_v110_revI_10Nov2020EnglishMinion Download 06th July 2022 - Updated the snippet: "Prepare the NEBNext FFPE DNA Repair Mix and NEBNext Ultra II End repair / dA-tailing Module reagents in accordance with manufacturer’s instructions, and place on ice." to also include the manufacturers recommendations. - Removed reference to Kit 12 chemistry in the "Priming and loading the SpotON flow cell for GridION" section of the protocol.
July 6th, 2022LWP_9117_v110_revH_10Nov2020EnglishGridion Download 06th July 2022 - Updated the snippet: "Prepare the NEBNext FFPE DNA Repair Mix and NEBNext Ultra II End repair / dA-tailing Module reagents in accordance with manufacturer’s instructions, and place on ice." to also include the manufacturers recommendations.
July 6th, 2022LWP_9117_v110_revH_10Nov2020EnglishMinion Download 06th July 2022 - Updated the snippet: "Prepare the NEBNext FFPE DNA Repair Mix and NEBNext Ultra II End repair / dA-tailing Module reagents in accordance with manufacturer’s instructions, and place on ice." to also include the manufacturers recommendations.
July 6th, 2022LWP_9117_v110_revH_06Jul2022EnglishGridion Download 06th July 2022 - Updated the snippet: "Prepare the NEBNext FFPE DNA Repair Mix and NEBNext Ultra II End repair / dA-tailing Module reagents in accordance with manufacturer’s instructions, and place on ice." to also include the manufacturers recommendations.
July 6th, 2022LWP_9117_v110_revH_06Jul2022EnglishMinion Download 06th July 2022 - Updated the snippet: "Prepare the NEBNext FFPE DNA Repair Mix and NEBNext Ultra II End repair / dA-tailing Module reagents in accordance with manufacturer’s instructions, and place on ice." to also include the manufacturers recommendations.
July 4th, 2022LWP_9117_v110_revG_10Nov2020EnglishGridion Download July 2022 - Update the priming and loading the SpotON flow cells for both MinION and GridION with new diagrams.
July 4th, 2022LWP_9117_v110_revG_10Nov2020EnglishMinion Download July 2022 - Update the priming and loading the SpotON flow cells for both MinION and GridION with new diagrams.
April 13th, 2022LWP_9117_v110_revF_10Nov2020EnglishGridion Download 14th April 2022 Protocol name change
April 13th, 2022LWP_9117_v110_revF_10Nov2020EnglishMinion Download 14th April 2022 Protocol name change
February 24th, 2022LWP_9117_v110_revE_10Nov2020EnglishGridion Download 24th Febrary 2022 Updated EXP-AUX110 to new name EXP-AUX002
February 24th, 2022LWP_9117_v110_revE_10Nov2020EnglishMinion Download 24th Febrary 2022 Updated EXP-AUX110 to new name EXP-AUX002
January 26th, 2022LWP_9117_v110_revD_10Nov2020EnglishGridion Download 26th January 2022 - Updated mixing recommendations and adding tips regarding short term storage of the DNA library during the prep
January 26th, 2022LWP_9117_v110_revD_10Nov2020EnglishMinion Download 26th January 2022 - Updated mixing recommendations and adding tips regarding short term storage of the DNA library during the prep
September 13th, 2021LWP_9117_v110_revC_10Nov2020EnglishGridion Download 13th Sept 2021 - Added table outlining kit contents in the Equipment and Consumables section.
September 13th, 2021LWP_9117_v110_revC_10Nov2020EnglishMinion Download 13th Sept 2021 - Added table outlining kit contents in the Equipment and Consumables section.
January 20th, 2021LWP_9117_v110_revB_10Nov2020EnglishGridion Download 9th Dec Compatibilities updated for EXP-WSH004
January 20th, 2021LWP_9117_v110_revB_10Nov2020EnglishMinion Download 9th Dec Compatibilities updated for EXP-WSH004
November 10th, 2020LWP_9117_v110_revA_10Nov2020EnglishGridion Download -
November 10th, 2020LWP_9117_v110_revA_10Nov2020EnglishMinion Download -
October 20th, 2020LWI_gDNA_SQK-LSK110_v1EnglishGridion Download -
October 20th, 2020LWI_gDNA_SQK-LSK110_v1EnglishMinion Download -
October 20th, 2020LWI_gDNA_SQK-LSK110_v1EnglishAny Download -