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Can I mix legacy and V14 kits on R9.4.1 and R10.4.1 flow cells?

Can I mix legacy and V14 kits on R9.4.1 and R10.4.1 flow cells?

No!

The V14 chemistry kits (such as SQK-LSK114) can only be run on R10.4.1 flow cells: FLO-FLG114 for Flongle, FLO-MIN114 for MinION / GridION and FLO-PRO114M for PromethION.

The legacy chemistry kits prior to V14 can only be run on R9.4.1 flow cells.

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