Resources: Documentation

10X Genomics Visium Spatial transcriptomics method: - Requires cDNA amplicons produced using the Visium Spatial Gene Expression assay (Visium V1 for Fresh Frozen) or the Visium HD 3' Spatial Gene Expression assay - Library preparation time ~205 minutes - High output - PCR required For Research Use Only

Single-cell transcriptomics method: - Requires cDNA amplicons produced using the 10x Genomics Chromium GEM-X Universal 3’ Gene Expression (V4) assay or the Chromium Next GEM Universal 3’ Gene Expression (V3.1) assay. - Library preparation time ~205 minutes - High output - PCR required For Research Use Only

The fastest and simplest protocol for full-length cDNA sequencing - Offering highest yield - Higher yields than traditional cDNA synthesis - Splice variants and fusion transcripts - Compatible with R10.4.1 flow cells <br> For Research Use Only __This is an Early Access product__ __For more information about our Early Access programmes, please see [this article on product release phases](https://nanoporetech.com/about-us/news/blog-you-cant-put-label-innovation-or-can-you).__

The fastest and simplest protocol for full-length cDNA sequencing - Offering highest yield - Higher yields than traditional cDNA synthesis - Splice variants and fusion transcripts - Multiplex up to 24 different samples - Compatible with R10.4.1 flow cells only <br> For Research Use Only __This is an Early Access product__ __For more information about our Early Access programmes, please see [this article on product release phases](https://nanoporetech.com/about-us/news/blog-you-cant-put-label-innovation-or-can-you).__

Single-cell transcriptomics method: - Requires cDNA amplicons produced using the 10x Genomics Chromium GEM-X Universal 5' Gene Expression (V3) assay or the Chromium Next GEM Universal 5' Gene Expression (V2) assay - Library preparation time ~135 minutes - High output - PCR required For Research Use Only

The PCR-free protocol for full-length cDNA offers: - Higher yields than traditional cDNA synthesis - Analysis of splice variants & fusion transcripts - Compatibility with R10.4.1 flow cells For Research Use Only

For Research Use Only

This protocol: - Is for sequencing native RNA - Can be used with total RNA or an enriched sample (e.g. poly(A) tailed or ribo-depleted) as a starting input material - Requires no fragmentation - Takes approximately 140 minutes for library preparation - Is only compatible with RNA flow cells __For Research Use Only__

This protocol: - Is for sequencing native RNA - Can be used with total RNA or an enriched sample (e.g. poly(A) tailed or ribo-depleted) as a starting input material - Requires no fragmentation - Takes approximately 140 minutes for library preparation - Is only compatible with RNA flow cells __For Research Use Only__

This protocol: - Is for a control experiment using kit-supplied RNA - Sequences native RNA - Requires no fragmentation - Takes approximately 140 minutes for library preparation - Is only compatible with RNA flow cells __For Research Use Only__