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Nanopore-only Microbial Isolate Sequencing Solution (NO-MISS) from cell cultures using SQK-RBK114 (.24 or .96) (ISO_9205_v114_revG_09May2025)

DateVersionLanguageDeviceChanges
6/23/2025ISO_9205_v114_revG_09May2025EnglishGridION
09 May 2025 - Protocol title update
6/23/2025ISO_9205_v114_revG_09May2025EnglishMinION
09 May 2025 - Protocol title update
12/13/2024ISO_9205_v114_revF_13Dec2024EnglishGridION
13 December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/13/2024ISO_9205_v114_revF_13Dec2024EnglishMinION
13 December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
11/15/2024ISO_9205_v114_revE_20Nov2024EnglishGridION
November 2024 - Updated kit content information and image to include new kit format for SQK-RBK114.24 with plated rapid barcodes. 14th October 2024 - Updated content nomenclature to reflect the updated product name for Monarch Genomic DNA Purification Kit #T3010. This has now changed to Monarch® Spin gDNA Extraction Kit #T3010. - Components within the Monarch® Spin gDNA Extraction Kit #T3010 have also had updated names and product codes: | Old name and code | New name/subcomponent part number | | --- | --- | | Monarch® gDNA Purification Columns T3017-1 | Monarch® Spin Columns S2C and Tubes T3017-1 | | Monarch® Collection Tubes II T2018-1 | Monarch® Spin Collection Tubes T2118-1 | | Proteinase K, Molecular Biology Grade P8107AAVIAL | Proteinase K, Molecular Biology Grade  P8200AAVIAL |
11/15/2024ISO_9205_v114_revE_20Nov2024EnglishMinION
November 2024 - Updated kit content information and image to include new kit format for SQK-RBK114.24 with plated rapid barcodes. 14th October 2024 - Updated content nomenclature to reflect the updated product name for Monarch Genomic DNA Purification Kit #T3010. This has now changed to Monarch® Spin gDNA Extraction Kit #T3010. - Components within the Monarch® Spin gDNA Extraction Kit #T3010 have also had updated names and product codes: | Old name and code | New name/subcomponent part number | | --- | --- | | Monarch® gDNA Purification Columns T3017-1 | Monarch® Spin Columns S2C and Tubes T3017-1 | | Monarch® Collection Tubes II T2018-1 | Monarch® Spin Collection Tubes T2118-1 | | Proteinase K, Molecular Biology Grade P8107AAVIAL | Proteinase K, Molecular Biology Grade  P8200AAVIAL |
10/14/2024ISO_9205_v114_revD_14Oct2024EnglishGridION
14th October 2024 - Updated content nomenclature to reflect the updated product name for Monarch Genomic DNA Purification Kit #T3010. This has now changed to Monarch® Spin gDNA Extraction Kit #T3010. - Components within the Monarch® Spin gDNA Extraction Kit #T3010 have also had updated names and product codes: | Old name and code | New name/subcomponent part number | | --- | --- | | Monarch® gDNA Purification Columns T3017-1 | Monarch® Spin Columns S2C and Tubes T3017-1 | | Monarch® Collection Tubes II T2018-1 | Monarch® Spin Collection Tubes T2118-1 | | Proteinase K, Molecular Biology Grade P8107AAVIAL | Proteinase K, Molecular Biology Grade  P8200AAVIAL |
10/14/2024ISO_9205_v114_revD_14Oct2024EnglishMinION
14th October 2024 - Updated content nomenclature to reflect the updated product name for Monarch Genomic DNA Purification Kit #T3010. This has now changed to Monarch® Spin gDNA Extraction Kit #T3010. - Components within the Monarch® Spin gDNA Extraction Kit #T3010 have also had updated names and product codes: | Old name and code | New name/subcomponent part number | | --- | --- | | Monarch® gDNA Purification Columns T3017-1 | Monarch® Spin Columns S2C and Tubes T3017-1 | | Monarch® Collection Tubes II T2018-1 | Monarch® Spin Collection Tubes T2118-1 | | Proteinase K, Molecular Biology Grade P8107AAVIAL | Proteinase K, Molecular Biology Grade  P8200AAVIAL |
9/30/2024ISO_9205_v114_revC_30Sept2024EnglishGridION
30th September 2024 - Moved protocol out of early access phase, removed early access wording.
9/30/2024ISO_9205_v114_revC_30Sept2024EnglishMinION
30th September 2024 - Moved protocol out of early access phase, removed early access wording.
5/14/2024ISO_9205_v114_revB_20Mar2024EnglishGridION
27th March 2024 - Updated wording for MinKNOW updates to specify v24.02 or newer
5/14/2024ISO_9205_v114_revB_20Mar2024EnglishMinION
27th March 2024 - Updated wording for MinKNOW updates to specify v24.02 or newer
May 14th, 2024ISO_v9205_v114_revB_20Mar2024EnglishGridion Download 27th March 2024 - Updated wording for MinKNOW updates to specify v24.02 or newer
May 14th, 2024ISO_v9205_v114_revB_20Mar2024EnglishMinion Download 27th March 2024 - Updated wording for MinKNOW updates to specify v24.02 or newer
March 20th, 2024ISO_v9205_v114_revA_20Mar2024EnglishGridion Download 20th March 2024 Protocol released
March 20th, 2024ISO_v9205_v114_revA_20Mar2024EnglishMinion Download 20th March 2024 Protocol released

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