DNA连接法建库 V14（SQK-LSK114） (GDE_9161_v114_revAA_30May2025)

DateVersionLanguageDeviceChanges
6/9/2025GDE_9161_v114_revAA_30May2025EnglishFlongle
30th May 2025 - In 'Equipment and consumables' image of kit in vial format was removed. - In section 3, 'DNA repair and end-prep', step 2, Ultra II End Prep Reaction Buffer was removed from MinION and PromethION branches as it is not included in the Table in step 4.
6/9/2025GDE_9161_v114_revAA_30May2025EnglishGridION
30th May 2025 - In 'Equipment and consumables' image of kit in vial format was removed. - In section 3, 'DNA repair and end-prep', step 2, Ultra II End Prep Reaction Buffer was removed from MinION and PromethION branches as it is not included in the Table in step 4.
6/9/2025GDE_9161_v114_revAA_30May2025EnglishMinION
30th May 2025 - In 'Equipment and consumables' image of kit in vial format was removed. - In section 3, 'DNA repair and end-prep', step 2, Ultra II End Prep Reaction Buffer was removed from MinION and PromethION branches as it is not included in the Table in step 4.
6/9/2025GDE_9161_v114_revAA_30May2025EnglishPromethION
30th May 2025 - In 'Equipment and consumables' image of kit in vial format was removed. - In section 3, 'DNA repair and end-prep', step 2, Ultra II End Prep Reaction Buffer was removed from MinION and PromethION branches as it is not included in the Table in step 4.
6/9/2025GDE_9161_v114_revAA_30May2025EspañolMinION
30th May 2025 - In 'Equipment and consumables' image of kit in vial format was removed. - In section 3, 'DNA repair and end-prep', step 2, Ultra II End Prep Reaction Buffer was removed from MinION and PromethION branches as it is not included in the Table in step 4.
6/9/2025GDE_9161_v114_revAA_30May2025EspañolPromethION
30th May 2025 - In 'Equipment and consumables' image of kit in vial format was removed. - In section 3, 'DNA repair and end-prep', step 2, Ultra II End Prep Reaction Buffer was removed from MinION and PromethION branches as it is not included in the Table in step 4.
6/9/2025GDE_9161_v114_revAA_30May2025日本Flongle
30th May 2025 - In 'Equipment and consumables' image of kit in vial format was removed. - In section 3, 'DNA repair and end-prep', step 2, Ultra II End Prep Reaction Buffer was removed from MinION and PromethION branches as it is not included in the Table in step 4.
6/9/2025GDE_9161_v114_revAA_30May2025日本GridION
30th May 2025 - In 'Equipment and consumables' image of kit in vial format was removed. - In section 3, 'DNA repair and end-prep', step 2, Ultra II End Prep Reaction Buffer was removed from MinION and PromethION branches as it is not included in the Table in step 4.
6/9/2025GDE_9161_v114_revAA_30May2025日本MinION
30th May 2025 - In 'Equipment and consumables' image of kit in vial format was removed. - In section 3, 'DNA repair and end-prep', step 2, Ultra II End Prep Reaction Buffer was removed from MinION and PromethION branches as it is not included in the Table in step 4.
6/9/2025GDE_9161_v114_revAA_30May2025日本PromethION
30th May 2025 - In 'Equipment and consumables' image of kit in vial format was removed. - In section 3, 'DNA repair and end-prep', step 2, Ultra II End Prep Reaction Buffer was removed from MinION and PromethION branches as it is not included in the Table in step 4.
6/9/2025GDE_9161_v114_revAA_30May2025中文MinION
30th May 2025 - In 'Equipment and consumables' image of kit in vial format was removed. - In section 3, 'DNA repair and end-prep', step 2, Ultra II End Prep Reaction Buffer was removed from MinION and PromethION branches as it is not included in the Table in step 4.
6/9/2025GDE_9161_v114_revAA_30May2025中文PromethION
30th May 2025 - In 'Equipment and consumables' image of kit in vial format was removed. - In section 3, 'DNA repair and end-prep', step 2, Ultra II End Prep Reaction Buffer was removed from MinION and PromethION branches as it is not included in the Table in step 4.
5/30/2025GDE_9161_v114_revAA_30May2025EnglishFlongle
30th May 2025 - In 'Equipment and consumables' image of kit in vial format was removed. - In section 3, 'DNA repair and end-prep', step 2, Ultra II End Prep Reaction Buffer was removed from MinION and PromethION branches as it is not included in the Table in step 4.
5/30/2025GDE_9161_v114_revAA_30May2025EnglishGridION
30th May 2025 - In 'Equipment and consumables' image of kit in vial format was removed. - In section 3, 'DNA repair and end-prep', step 2, Ultra II End Prep Reaction Buffer was removed from MinION and PromethION branches as it is not included in the Table in step 4.
5/30/2025GDE_9161_v114_revAA_30May2025EnglishMinION
30th May 2025 - In 'Equipment and consumables' image of kit in vial format was removed. - In section 3, 'DNA repair and end-prep', step 2, Ultra II End Prep Reaction Buffer was removed from MinION and PromethION branches as it is not included in the Table in step 4.
5/30/2025GDE_9161_v114_revAA_30May2025EnglishPromethION
30th May 2025 - In 'Equipment and consumables' image of kit in vial format was removed. - In section 3, 'DNA repair and end-prep', step 2, Ultra II End Prep Reaction Buffer was removed from MinION and PromethION branches as it is not included in the Table in step 4.
5/30/2025GDE_9161_v114_revAA_30May2025EspañolMinION
30th May 2025 - In 'Equipment and consumables' image of kit in vial format was removed. - In section 3, 'DNA repair and end-prep', step 2, Ultra II End Prep Reaction Buffer was removed from MinION and PromethION branches as it is not included in the Table in step 4.
5/30/2025GDE_9161_v114_revAA_30May2025EspañolPromethION
30th May 2025 - In 'Equipment and consumables' image of kit in vial format was removed. - In section 3, 'DNA repair and end-prep', step 2, Ultra II End Prep Reaction Buffer was removed from MinION and PromethION branches as it is not included in the Table in step 4.
5/30/2025GDE_9161_v114_revAA_30May2025日本Flongle
30th May 2025 - In 'Equipment and consumables' image of kit in vial format was removed. - In section 3, 'DNA repair and end-prep', step 2, Ultra II End Prep Reaction Buffer was removed from MinION and PromethION branches as it is not included in the Table in step 4.
5/30/2025GDE_9161_v114_revAA_30May2025日本GridION
30th May 2025 - In 'Equipment and consumables' image of kit in vial format was removed. - In section 3, 'DNA repair and end-prep', step 2, Ultra II End Prep Reaction Buffer was removed from MinION and PromethION branches as it is not included in the Table in step 4.
5/30/2025GDE_9161_v114_revAA_30May2025日本MinION
30th May 2025 - In 'Equipment and consumables' image of kit in vial format was removed. - In section 3, 'DNA repair and end-prep', step 2, Ultra II End Prep Reaction Buffer was removed from MinION and PromethION branches as it is not included in the Table in step 4.
5/30/2025GDE_9161_v114_revAA_30May2025日本PromethION
30th May 2025 - In 'Equipment and consumables' image of kit in vial format was removed. - In section 3, 'DNA repair and end-prep', step 2, Ultra II End Prep Reaction Buffer was removed from MinION and PromethION branches as it is not included in the Table in step 4.
5/30/2025GDE_9161_v114_revAA_30May2025中文MinION
30th May 2025 - In 'Equipment and consumables' image of kit in vial format was removed. - In section 3, 'DNA repair and end-prep', step 2, Ultra II End Prep Reaction Buffer was removed from MinION and PromethION branches as it is not included in the Table in step 4.
5/30/2025GDE_9161_v114_revAA_30May2025中文PromethION
30th May 2025 - In 'Equipment and consumables' image of kit in vial format was removed. - In section 3, 'DNA repair and end-prep', step 2, Ultra II End Prep Reaction Buffer was removed from MinION and PromethION branches as it is not included in the Table in step 4.
5/8/2025GDE_9161_v114_revZ_08May2025EnglishFlongle
8th May 2025 - In 'Overview', library preparation time was increased to ~65 min to align with the Table and Figure in 'Steps in the sequencing workflow' where the priming and loading of flow cell was increased from 5 min to 10 min. - In section 1, 'Overview of the protocol', 'Introduction to the Ligation Sequencing Kit V14 (SQK-LSK114) protocol', the snippet setting was changed from being a Tip (green box) to Information. - In section 3, 'DNA repair and end-prep, step 2, point 4, the previous 'FFPE DNA Repair Buffer and Ultra II End Prep Reaction Buffer ' were removed from the MinION/GridION and PromethION branches, as it is preferable to use the latest 'FFPE DNA Repair Buffer v2'. And in step 6, the following was added to all device arms: 'Then cool down to between 4°C and 20°C on the thermal cycler or place the samples on ice.'. - In section 5, 'Priming and loading the MinION and GridION Flow Cell' and 'Flongle Flow Cell loading', an important note was added to the bottom of the section stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device. - For the PromethION branch, the step for removing the PromethION Flow Cell out of the fridge was moved to the beginning of the section on 'Priming and loading the PromethION Flow Cell' to enable the flow cell to reach room temperature while the flow cell priming mix is prepared. And an optional action of performing a flow cell check was included. - In section 6, 'Data acquisition and basecalling', 'To start a sequencing run in MinKNOW', the sequencing kit was specified in point 3, 'Select the Ligation Sequencing Kit V14 (SQK-LSK114) on the Kit page'. And the section on 'Duplex basecalling' was removed as it is no longer applicable. - For the Flongle branch, the sections on 'Flow cell deterioration/saturation' and 'Flongle Sequencing Expansion' were edited. - The video link for priming and loading a Flongle Flow Cell was updated in section 5, step 4. - Screenshots were removed in section 6, 'Data acquisition and basecalling'.
5/8/2025GDE_9161_v114_revZ_08May2025EnglishGridION
8th May 2025 - In 'Overview', library preparation time was increased to ~65 min to align with the Table and Figure in 'Steps in the sequencing workflow' where the priming and loading of flow cell was increased from 5 min to 10 min. - In section 1, 'Overview of the protocol', 'Introduction to the Ligation Sequencing Kit V14 (SQK-LSK114) protocol', the snippet setting was changed from being a Tip (green box) to Information. - In section 3, 'DNA repair and end-prep, step 2, point 4, the previous 'FFPE DNA Repair Buffer and Ultra II End Prep Reaction Buffer ' were removed from the MinION/GridION and PromethION branches, as it is preferable to use the latest 'FFPE DNA Repair Buffer v2'. And in step 6, the following was added to all device arms: 'Then cool down to between 4°C and 20°C on the thermal cycler or place the samples on ice.'. - In section 5, 'Priming and loading the MinION and GridION Flow Cell' and 'Flongle Flow Cell loading', an important note was added to the bottom of the section stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device. - For the PromethION branch, the step for removing the PromethION Flow Cell out of the fridge was moved to the beginning of the section on 'Priming and loading the PromethION Flow Cell' to enable the flow cell to reach room temperature while the flow cell priming mix is prepared. And an optional action of performing a flow cell check was included. - In section 6, 'Data acquisition and basecalling', 'To start a sequencing run in MinKNOW', the sequencing kit was specified in point 3, 'Select the Ligation Sequencing Kit V14 (SQK-LSK114) on the Kit page'. And the section on 'Duplex basecalling' was removed as it is no longer applicable. - For the Flongle branch, the sections on 'Flow cell deterioration/saturation' and 'Flongle Sequencing Expansion' were edited. - The video link for priming and loading a Flongle Flow Cell was updated in section 5, step 4. - Screenshots were removed in section 6, 'Data acquisition and basecalling'.
5/8/2025GDE_9161_v114_revZ_08May2025EnglishMinION
8th May 2025 - In 'Overview', library preparation time was increased to ~65 min to align with the Table and Figure in 'Steps in the sequencing workflow' where the priming and loading of flow cell was increased from 5 min to 10 min. - In section 1, 'Overview of the protocol', 'Introduction to the Ligation Sequencing Kit V14 (SQK-LSK114) protocol', the snippet setting was changed from being a Tip (green box) to Information. - In section 3, 'DNA repair and end-prep, step 2, point 4, the previous 'FFPE DNA Repair Buffer and Ultra II End Prep Reaction Buffer ' were removed from the MinION/GridION and PromethION branches, as it is preferable to use the latest 'FFPE DNA Repair Buffer v2'. And in step 6, the following was added to all device arms: 'Then cool down to between 4°C and 20°C on the thermal cycler or place the samples on ice.'. - In section 5, 'Priming and loading the MinION and GridION Flow Cell' and 'Flongle Flow Cell loading', an important note was added to the bottom of the section stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device. - For the PromethION branch, the step for removing the PromethION Flow Cell out of the fridge was moved to the beginning of the section on 'Priming and loading the PromethION Flow Cell' to enable the flow cell to reach room temperature while the flow cell priming mix is prepared. And an optional action of performing a flow cell check was included. - In section 6, 'Data acquisition and basecalling', 'To start a sequencing run in MinKNOW', the sequencing kit was specified in point 3, 'Select the Ligation Sequencing Kit V14 (SQK-LSK114) on the Kit page'. And the section on 'Duplex basecalling' was removed as it is no longer applicable. - For the Flongle branch, the sections on 'Flow cell deterioration/saturation' and 'Flongle Sequencing Expansion' were edited. - The video link for priming and loading a Flongle Flow Cell was updated in section 5, step 4. - Screenshots were removed in section 6, 'Data acquisition and basecalling'.
5/8/2025GDE_9161_v114_revZ_08May2025EnglishPromethION
8th May 2025 - In 'Overview', library preparation time was increased to ~65 min to align with the Table and Figure in 'Steps in the sequencing workflow' where the priming and loading of flow cell was increased from 5 min to 10 min. - In section 1, 'Overview of the protocol', 'Introduction to the Ligation Sequencing Kit V14 (SQK-LSK114) protocol', the snippet setting was changed from being a Tip (green box) to Information. - In section 3, 'DNA repair and end-prep, step 2, point 4, the previous 'FFPE DNA Repair Buffer and Ultra II End Prep Reaction Buffer ' were removed from the MinION/GridION and PromethION branches, as it is preferable to use the latest 'FFPE DNA Repair Buffer v2'. And in step 6, the following was added to all device arms: 'Then cool down to between 4°C and 20°C on the thermal cycler or place the samples on ice.'. - In section 5, 'Priming and loading the MinION and GridION Flow Cell' and 'Flongle Flow Cell loading', an important note was added to the bottom of the section stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device. - For the PromethION branch, the step for removing the PromethION Flow Cell out of the fridge was moved to the beginning of the section on 'Priming and loading the PromethION Flow Cell' to enable the flow cell to reach room temperature while the flow cell priming mix is prepared. And an optional action of performing a flow cell check was included. - In section 6, 'Data acquisition and basecalling', 'To start a sequencing run in MinKNOW', the sequencing kit was specified in point 3, 'Select the Ligation Sequencing Kit V14 (SQK-LSK114) on the Kit page'. And the section on 'Duplex basecalling' was removed as it is no longer applicable. - For the Flongle branch, the sections on 'Flow cell deterioration/saturation' and 'Flongle Sequencing Expansion' were edited. - The video link for priming and loading a Flongle Flow Cell was updated in section 5, step 4. - Screenshots were removed in section 6, 'Data acquisition and basecalling'.
5/8/2025GDE_9161_v114_revZ_08May2025EspañolMinION
8th May 2025 - In 'Overview', library preparation time was increased to ~65 min to align with the Table and Figure in 'Steps in the sequencing workflow' where the priming and loading of flow cell was increased from 5 min to 10 min. - In section 1, 'Overview of the protocol', 'Introduction to the Ligation Sequencing Kit V14 (SQK-LSK114) protocol', the snippet setting was changed from being a Tip (green box) to Information. - In section 3, 'DNA repair and end-prep, step 2, point 4, the previous 'FFPE DNA Repair Buffer and Ultra II End Prep Reaction Buffer ' were removed from the MinION/GridION and PromethION branches, as it is preferable to use the latest 'FFPE DNA Repair Buffer v2'. And in step 6, the following was added to all device arms: 'Then cool down to between 4°C and 20°C on the thermal cycler or place the samples on ice.'. - In section 5, 'Priming and loading the MinION and GridION Flow Cell' and 'Flongle Flow Cell loading', an important note was added to the bottom of the section stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device. - For the PromethION branch, the step for removing the PromethION Flow Cell out of the fridge was moved to the beginning of the section on 'Priming and loading the PromethION Flow Cell' to enable the flow cell to reach room temperature while the flow cell priming mix is prepared. And an optional action of performing a flow cell check was included. - In section 6, 'Data acquisition and basecalling', 'To start a sequencing run in MinKNOW', the sequencing kit was specified in point 3, 'Select the Ligation Sequencing Kit V14 (SQK-LSK114) on the Kit page'. And the section on 'Duplex basecalling' was removed as it is no longer applicable. - For the Flongle branch, the sections on 'Flow cell deterioration/saturation' and 'Flongle Sequencing Expansion' were edited. - The video link for priming and loading a Flongle Flow Cell was updated in section 5, step 4. - Screenshots were removed in section 6, 'Data acquisition and basecalling'.
5/8/2025GDE_9161_v114_revZ_08May2025EspañolPromethION
8th May 2025 - In 'Overview', library preparation time was increased to ~65 min to align with the Table and Figure in 'Steps in the sequencing workflow' where the priming and loading of flow cell was increased from 5 min to 10 min. - In section 1, 'Overview of the protocol', 'Introduction to the Ligation Sequencing Kit V14 (SQK-LSK114) protocol', the snippet setting was changed from being a Tip (green box) to Information. - In section 3, 'DNA repair and end-prep, step 2, point 4, the previous 'FFPE DNA Repair Buffer and Ultra II End Prep Reaction Buffer ' were removed from the MinION/GridION and PromethION branches, as it is preferable to use the latest 'FFPE DNA Repair Buffer v2'. And in step 6, the following was added to all device arms: 'Then cool down to between 4°C and 20°C on the thermal cycler or place the samples on ice.'. - In section 5, 'Priming and loading the MinION and GridION Flow Cell' and 'Flongle Flow Cell loading', an important note was added to the bottom of the section stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device. - For the PromethION branch, the step for removing the PromethION Flow Cell out of the fridge was moved to the beginning of the section on 'Priming and loading the PromethION Flow Cell' to enable the flow cell to reach room temperature while the flow cell priming mix is prepared. And an optional action of performing a flow cell check was included. - In section 6, 'Data acquisition and basecalling', 'To start a sequencing run in MinKNOW', the sequencing kit was specified in point 3, 'Select the Ligation Sequencing Kit V14 (SQK-LSK114) on the Kit page'. And the section on 'Duplex basecalling' was removed as it is no longer applicable. - For the Flongle branch, the sections on 'Flow cell deterioration/saturation' and 'Flongle Sequencing Expansion' were edited. - The video link for priming and loading a Flongle Flow Cell was updated in section 5, step 4. - Screenshots were removed in section 6, 'Data acquisition and basecalling'.
5/8/2025GDE_9161_v114_revZ_08May2025日本Flongle
8th May 2025 - In 'Overview', library preparation time was increased to ~65 min to align with the Table and Figure in 'Steps in the sequencing workflow' where the priming and loading of flow cell was increased from 5 min to 10 min. - In section 1, 'Overview of the protocol', 'Introduction to the Ligation Sequencing Kit V14 (SQK-LSK114) protocol', the snippet setting was changed from being a Tip (green box) to Information. - In section 3, 'DNA repair and end-prep, step 2, point 4, the previous 'FFPE DNA Repair Buffer and Ultra II End Prep Reaction Buffer ' were removed from the MinION/GridION and PromethION branches, as it is preferable to use the latest 'FFPE DNA Repair Buffer v2'. And in step 6, the following was added to all device arms: 'Then cool down to between 4°C and 20°C on the thermal cycler or place the samples on ice.'. - In section 5, 'Priming and loading the MinION and GridION Flow Cell' and 'Flongle Flow Cell loading', an important note was added to the bottom of the section stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device. - For the PromethION branch, the step for removing the PromethION Flow Cell out of the fridge was moved to the beginning of the section on 'Priming and loading the PromethION Flow Cell' to enable the flow cell to reach room temperature while the flow cell priming mix is prepared. And an optional action of performing a flow cell check was included. - In section 6, 'Data acquisition and basecalling', 'To start a sequencing run in MinKNOW', the sequencing kit was specified in point 3, 'Select the Ligation Sequencing Kit V14 (SQK-LSK114) on the Kit page'. And the section on 'Duplex basecalling' was removed as it is no longer applicable. - For the Flongle branch, the sections on 'Flow cell deterioration/saturation' and 'Flongle Sequencing Expansion' were edited. - The video link for priming and loading a Flongle Flow Cell was updated in section 5, step 4. - Screenshots were removed in section 6, 'Data acquisition and basecalling'.
5/8/2025GDE_9161_v114_revZ_08May2025日本GridION
8th May 2025 - In 'Overview', library preparation time was increased to ~65 min to align with the Table and Figure in 'Steps in the sequencing workflow' where the priming and loading of flow cell was increased from 5 min to 10 min. - In section 1, 'Overview of the protocol', 'Introduction to the Ligation Sequencing Kit V14 (SQK-LSK114) protocol', the snippet setting was changed from being a Tip (green box) to Information. - In section 3, 'DNA repair and end-prep, step 2, point 4, the previous 'FFPE DNA Repair Buffer and Ultra II End Prep Reaction Buffer ' were removed from the MinION/GridION and PromethION branches, as it is preferable to use the latest 'FFPE DNA Repair Buffer v2'. And in step 6, the following was added to all device arms: 'Then cool down to between 4°C and 20°C on the thermal cycler or place the samples on ice.'. - In section 5, 'Priming and loading the MinION and GridION Flow Cell' and 'Flongle Flow Cell loading', an important note was added to the bottom of the section stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device. - For the PromethION branch, the step for removing the PromethION Flow Cell out of the fridge was moved to the beginning of the section on 'Priming and loading the PromethION Flow Cell' to enable the flow cell to reach room temperature while the flow cell priming mix is prepared. And an optional action of performing a flow cell check was included. - In section 6, 'Data acquisition and basecalling', 'To start a sequencing run in MinKNOW', the sequencing kit was specified in point 3, 'Select the Ligation Sequencing Kit V14 (SQK-LSK114) on the Kit page'. And the section on 'Duplex basecalling' was removed as it is no longer applicable. - For the Flongle branch, the sections on 'Flow cell deterioration/saturation' and 'Flongle Sequencing Expansion' were edited. - The video link for priming and loading a Flongle Flow Cell was updated in section 5, step 4. - Screenshots were removed in section 6, 'Data acquisition and basecalling'.
5/8/2025GDE_9161_v114_revZ_08May2025日本MinION
8th May 2025 - In 'Overview', library preparation time was increased to ~65 min to align with the Table and Figure in 'Steps in the sequencing workflow' where the priming and loading of flow cell was increased from 5 min to 10 min. - In section 1, 'Overview of the protocol', 'Introduction to the Ligation Sequencing Kit V14 (SQK-LSK114) protocol', the snippet setting was changed from being a Tip (green box) to Information. - In section 3, 'DNA repair and end-prep, step 2, point 4, the previous 'FFPE DNA Repair Buffer and Ultra II End Prep Reaction Buffer ' were removed from the MinION/GridION and PromethION branches, as it is preferable to use the latest 'FFPE DNA Repair Buffer v2'. And in step 6, the following was added to all device arms: 'Then cool down to between 4°C and 20°C on the thermal cycler or place the samples on ice.'. - In section 5, 'Priming and loading the MinION and GridION Flow Cell' and 'Flongle Flow Cell loading', an important note was added to the bottom of the section stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device. - For the PromethION branch, the step for removing the PromethION Flow Cell out of the fridge was moved to the beginning of the section on 'Priming and loading the PromethION Flow Cell' to enable the flow cell to reach room temperature while the flow cell priming mix is prepared. And an optional action of performing a flow cell check was included. - In section 6, 'Data acquisition and basecalling', 'To start a sequencing run in MinKNOW', the sequencing kit was specified in point 3, 'Select the Ligation Sequencing Kit V14 (SQK-LSK114) on the Kit page'. And the section on 'Duplex basecalling' was removed as it is no longer applicable. - For the Flongle branch, the sections on 'Flow cell deterioration/saturation' and 'Flongle Sequencing Expansion' were edited. - The video link for priming and loading a Flongle Flow Cell was updated in section 5, step 4. - Screenshots were removed in section 6, 'Data acquisition and basecalling'.
5/8/2025GDE_9161_v114_revZ_08May2025日本PromethION
8th May 2025 - In 'Overview', library preparation time was increased to ~65 min to align with the Table and Figure in 'Steps in the sequencing workflow' where the priming and loading of flow cell was increased from 5 min to 10 min. - In section 1, 'Overview of the protocol', 'Introduction to the Ligation Sequencing Kit V14 (SQK-LSK114) protocol', the snippet setting was changed from being a Tip (green box) to Information. - In section 3, 'DNA repair and end-prep, step 2, point 4, the previous 'FFPE DNA Repair Buffer and Ultra II End Prep Reaction Buffer ' were removed from the MinION/GridION and PromethION branches, as it is preferable to use the latest 'FFPE DNA Repair Buffer v2'. And in step 6, the following was added to all device arms: 'Then cool down to between 4°C and 20°C on the thermal cycler or place the samples on ice.'. - In section 5, 'Priming and loading the MinION and GridION Flow Cell' and 'Flongle Flow Cell loading', an important note was added to the bottom of the section stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device. - For the PromethION branch, the step for removing the PromethION Flow Cell out of the fridge was moved to the beginning of the section on 'Priming and loading the PromethION Flow Cell' to enable the flow cell to reach room temperature while the flow cell priming mix is prepared. And an optional action of performing a flow cell check was included. - In section 6, 'Data acquisition and basecalling', 'To start a sequencing run in MinKNOW', the sequencing kit was specified in point 3, 'Select the Ligation Sequencing Kit V14 (SQK-LSK114) on the Kit page'. And the section on 'Duplex basecalling' was removed as it is no longer applicable. - For the Flongle branch, the sections on 'Flow cell deterioration/saturation' and 'Flongle Sequencing Expansion' were edited. - The video link for priming and loading a Flongle Flow Cell was updated in section 5, step 4. - Screenshots were removed in section 6, 'Data acquisition and basecalling'.
5/8/2025GDE_9161_v114_revZ_08May2025中文MinION
8th May 2025 - In 'Overview', library preparation time was increased to ~65 min to align with the Table and Figure in 'Steps in the sequencing workflow' where the priming and loading of flow cell was increased from 5 min to 10 min. - In section 1, 'Overview of the protocol', 'Introduction to the Ligation Sequencing Kit V14 (SQK-LSK114) protocol', the snippet setting was changed from being a Tip (green box) to Information. - In section 3, 'DNA repair and end-prep, step 2, point 4, the previous 'FFPE DNA Repair Buffer and Ultra II End Prep Reaction Buffer ' were removed from the MinION/GridION and PromethION branches, as it is preferable to use the latest 'FFPE DNA Repair Buffer v2'. And in step 6, the following was added to all device arms: 'Then cool down to between 4°C and 20°C on the thermal cycler or place the samples on ice.'. - In section 5, 'Priming and loading the MinION and GridION Flow Cell' and 'Flongle Flow Cell loading', an important note was added to the bottom of the section stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device. - For the PromethION branch, the step for removing the PromethION Flow Cell out of the fridge was moved to the beginning of the section on 'Priming and loading the PromethION Flow Cell' to enable the flow cell to reach room temperature while the flow cell priming mix is prepared. And an optional action of performing a flow cell check was included. - In section 6, 'Data acquisition and basecalling', 'To start a sequencing run in MinKNOW', the sequencing kit was specified in point 3, 'Select the Ligation Sequencing Kit V14 (SQK-LSK114) on the Kit page'. And the section on 'Duplex basecalling' was removed as it is no longer applicable. - For the Flongle branch, the sections on 'Flow cell deterioration/saturation' and 'Flongle Sequencing Expansion' were edited. - The video link for priming and loading a Flongle Flow Cell was updated in section 5, step 4. - Screenshots were removed in section 6, 'Data acquisition and basecalling'.
5/8/2025GDE_9161_v114_revZ_08May2025中文PromethION
8th May 2025 - In 'Overview', library preparation time was increased to ~65 min to align with the Table and Figure in 'Steps in the sequencing workflow' where the priming and loading of flow cell was increased from 5 min to 10 min. - In section 1, 'Overview of the protocol', 'Introduction to the Ligation Sequencing Kit V14 (SQK-LSK114) protocol', the snippet setting was changed from being a Tip (green box) to Information. - In section 3, 'DNA repair and end-prep, step 2, point 4, the previous 'FFPE DNA Repair Buffer and Ultra II End Prep Reaction Buffer ' were removed from the MinION/GridION and PromethION branches, as it is preferable to use the latest 'FFPE DNA Repair Buffer v2'. And in step 6, the following was added to all device arms: 'Then cool down to between 4°C and 20°C on the thermal cycler or place the samples on ice.'. - In section 5, 'Priming and loading the MinION and GridION Flow Cell' and 'Flongle Flow Cell loading', an important note was added to the bottom of the section stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device. - For the PromethION branch, the step for removing the PromethION Flow Cell out of the fridge was moved to the beginning of the section on 'Priming and loading the PromethION Flow Cell' to enable the flow cell to reach room temperature while the flow cell priming mix is prepared. And an optional action of performing a flow cell check was included. - In section 6, 'Data acquisition and basecalling', 'To start a sequencing run in MinKNOW', the sequencing kit was specified in point 3, 'Select the Ligation Sequencing Kit V14 (SQK-LSK114) on the Kit page'. And the section on 'Duplex basecalling' was removed as it is no longer applicable. - For the Flongle branch, the sections on 'Flow cell deterioration/saturation' and 'Flongle Sequencing Expansion' were edited. - The video link for priming and loading a Flongle Flow Cell was updated in section 5, step 4. - Screenshots were removed in section 6, 'Data acquisition and basecalling'.
5/22/2025GDE_9161_v114_revZ_08May2025EnglishFlongle
8th May 2025 - In 'Overview', library preparation time was increased to ~65 min to align with the Table and Figure in 'Steps in the sequencing workflow' where the priming and loading of flow cell was increased from 5 min to 10 min. - In section 1, 'Overview of the protocol', 'Introduction to the Ligation Sequencing Kit V14 (SQK-LSK114) protocol', the snippet setting was changed from being a Tip (green box) to Information. - In section 3, 'DNA repair and end-prep, step 2, point 4, the previous 'FFPE DNA Repair Buffer' and 'Ultra II End Prep Reaction Buffer' were removed from the MinION/GridION and PromethION branches, as it is preferable to use the latest 'FFPE DNA Repair Buffer v2'. And in step 6, the following was added to all device arms: 'Then cool down to between 4°C and 20°C on the thermal cycler or place the samples on ice.'. - In section 5, 'Priming and loading the MinION and GridION Flow Cell' and 'Flongle Flow Cell loading', an important note was added to the bottom of the section stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device. - For the PromethION branch, the step for removing the PromethION Flow Cell out of the fridge was moved to the beginning of the section on 'Priming and loading the PromethION Flow Cell' to enable the flow cell to reach room temperature while the flow cell priming mix is prepared. And an optional action of performing a flow cell check was included. - In section 6, 'Data acquisition and basecalling', 'To start a sequencing run in MinKNOW', the sequencing kit was specified in point 3, 'Select the Ligation Sequencing Kit V14 (SQK-LSK114) on the Kit page'. And the section on 'Duplex basecalling' was removed as it is no longer applicable. - For the Flongle branch, the sections on 'Flow cell deterioration/saturation' and 'Flongle Sequencing Expansion' were edited. - The video link for priming and loading a Flongle Flow Cell was updated in section 5, step 4. - Screenshots were removed in section 6, 'Data acquisition and basecalling'.
5/22/2025GDE_9161_v114_revZ_08May2025EnglishGridION
8th May 2025 - In 'Overview', library preparation time was increased to ~65 min to align with the Table and Figure in 'Steps in the sequencing workflow' where the priming and loading of flow cell was increased from 5 min to 10 min. - In section 1, 'Overview of the protocol', 'Introduction to the Ligation Sequencing Kit V14 (SQK-LSK114) protocol', the snippet setting was changed from being a Tip (green box) to Information. - In section 3, 'DNA repair and end-prep, step 2, point 4, the previous 'FFPE DNA Repair Buffer' and 'Ultra II End Prep Reaction Buffer' were removed from the MinION/GridION and PromethION branches, as it is preferable to use the latest 'FFPE DNA Repair Buffer v2'. And in step 6, the following was added to all device arms: 'Then cool down to between 4°C and 20°C on the thermal cycler or place the samples on ice.'. - In section 5, 'Priming and loading the MinION and GridION Flow Cell' and 'Flongle Flow Cell loading', an important note was added to the bottom of the section stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device. - For the PromethION branch, the step for removing the PromethION Flow Cell out of the fridge was moved to the beginning of the section on 'Priming and loading the PromethION Flow Cell' to enable the flow cell to reach room temperature while the flow cell priming mix is prepared. And an optional action of performing a flow cell check was included. - In section 6, 'Data acquisition and basecalling', 'To start a sequencing run in MinKNOW', the sequencing kit was specified in point 3, 'Select the Ligation Sequencing Kit V14 (SQK-LSK114) on the Kit page'. And the section on 'Duplex basecalling' was removed as it is no longer applicable. - For the Flongle branch, the sections on 'Flow cell deterioration/saturation' and 'Flongle Sequencing Expansion' were edited. - The video link for priming and loading a Flongle Flow Cell was updated in section 5, step 4. - Screenshots were removed in section 6, 'Data acquisition and basecalling'.
5/22/2025GDE_9161_v114_revZ_08May2025EnglishMinION
8th May 2025 - In 'Overview', library preparation time was increased to ~65 min to align with the Table and Figure in 'Steps in the sequencing workflow' where the priming and loading of flow cell was increased from 5 min to 10 min. - In section 1, 'Overview of the protocol', 'Introduction to the Ligation Sequencing Kit V14 (SQK-LSK114) protocol', the snippet setting was changed from being a Tip (green box) to Information. - In section 3, 'DNA repair and end-prep, step 2, point 4, the previous 'FFPE DNA Repair Buffer' and 'Ultra II End Prep Reaction Buffer' were removed from the MinION/GridION and PromethION branches, as it is preferable to use the latest 'FFPE DNA Repair Buffer v2'. And in step 6, the following was added to all device arms: 'Then cool down to between 4°C and 20°C on the thermal cycler or place the samples on ice.'. - In section 5, 'Priming and loading the MinION and GridION Flow Cell' and 'Flongle Flow Cell loading', an important note was added to the bottom of the section stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device. - For the PromethION branch, the step for removing the PromethION Flow Cell out of the fridge was moved to the beginning of the section on 'Priming and loading the PromethION Flow Cell' to enable the flow cell to reach room temperature while the flow cell priming mix is prepared. And an optional action of performing a flow cell check was included. - In section 6, 'Data acquisition and basecalling', 'To start a sequencing run in MinKNOW', the sequencing kit was specified in point 3, 'Select the Ligation Sequencing Kit V14 (SQK-LSK114) on the Kit page'. And the section on 'Duplex basecalling' was removed as it is no longer applicable. - For the Flongle branch, the sections on 'Flow cell deterioration/saturation' and 'Flongle Sequencing Expansion' were edited. - The video link for priming and loading a Flongle Flow Cell was updated in section 5, step 4. - Screenshots were removed in section 6, 'Data acquisition and basecalling'.
5/22/2025GDE_9161_v114_revZ_08May2025EnglishPromethION
8th May 2025 - In 'Overview', library preparation time was increased to ~65 min to align with the Table and Figure in 'Steps in the sequencing workflow' where the priming and loading of flow cell was increased from 5 min to 10 min. - In section 1, 'Overview of the protocol', 'Introduction to the Ligation Sequencing Kit V14 (SQK-LSK114) protocol', the snippet setting was changed from being a Tip (green box) to Information. - In section 3, 'DNA repair and end-prep, step 2, point 4, the previous 'FFPE DNA Repair Buffer' and 'Ultra II End Prep Reaction Buffer' were removed from the MinION/GridION and PromethION branches, as it is preferable to use the latest 'FFPE DNA Repair Buffer v2'. And in step 6, the following was added to all device arms: 'Then cool down to between 4°C and 20°C on the thermal cycler or place the samples on ice.'. - In section 5, 'Priming and loading the MinION and GridION Flow Cell' and 'Flongle Flow Cell loading', an important note was added to the bottom of the section stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device. - For the PromethION branch, the step for removing the PromethION Flow Cell out of the fridge was moved to the beginning of the section on 'Priming and loading the PromethION Flow Cell' to enable the flow cell to reach room temperature while the flow cell priming mix is prepared. And an optional action of performing a flow cell check was included. - In section 6, 'Data acquisition and basecalling', 'To start a sequencing run in MinKNOW', the sequencing kit was specified in point 3, 'Select the Ligation Sequencing Kit V14 (SQK-LSK114) on the Kit page'. And the section on 'Duplex basecalling' was removed as it is no longer applicable. - For the Flongle branch, the sections on 'Flow cell deterioration/saturation' and 'Flongle Sequencing Expansion' were edited. - The video link for priming and loading a Flongle Flow Cell was updated in section 5, step 4. - Screenshots were removed in section 6, 'Data acquisition and basecalling'.
5/22/2025GDE_9161_v114_revZ_08May2025EspañolMinION
8th May 2025 - In 'Overview', library preparation time was increased to ~65 min to align with the Table and Figure in 'Steps in the sequencing workflow' where the priming and loading of flow cell was increased from 5 min to 10 min. - In section 1, 'Overview of the protocol', 'Introduction to the Ligation Sequencing Kit V14 (SQK-LSK114) protocol', the snippet setting was changed from being a Tip (green box) to Information. - In section 3, 'DNA repair and end-prep, step 2, point 4, the previous 'FFPE DNA Repair Buffer' and 'Ultra II End Prep Reaction Buffer' were removed from the MinION/GridION and PromethION branches, as it is preferable to use the latest 'FFPE DNA Repair Buffer v2'. And in step 6, the following was added to all device arms: 'Then cool down to between 4°C and 20°C on the thermal cycler or place the samples on ice.'. - In section 5, 'Priming and loading the MinION and GridION Flow Cell' and 'Flongle Flow Cell loading', an important note was added to the bottom of the section stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device. - For the PromethION branch, the step for removing the PromethION Flow Cell out of the fridge was moved to the beginning of the section on 'Priming and loading the PromethION Flow Cell' to enable the flow cell to reach room temperature while the flow cell priming mix is prepared. And an optional action of performing a flow cell check was included. - In section 6, 'Data acquisition and basecalling', 'To start a sequencing run in MinKNOW', the sequencing kit was specified in point 3, 'Select the Ligation Sequencing Kit V14 (SQK-LSK114) on the Kit page'. And the section on 'Duplex basecalling' was removed as it is no longer applicable. - For the Flongle branch, the sections on 'Flow cell deterioration/saturation' and 'Flongle Sequencing Expansion' were edited. - The video link for priming and loading a Flongle Flow Cell was updated in section 5, step 4. - Screenshots were removed in section 6, 'Data acquisition and basecalling'.
5/22/2025GDE_9161_v114_revZ_08May2025EspañolPromethION
8th May 2025 - In 'Overview', library preparation time was increased to ~65 min to align with the Table and Figure in 'Steps in the sequencing workflow' where the priming and loading of flow cell was increased from 5 min to 10 min. - In section 1, 'Overview of the protocol', 'Introduction to the Ligation Sequencing Kit V14 (SQK-LSK114) protocol', the snippet setting was changed from being a Tip (green box) to Information. - In section 3, 'DNA repair and end-prep, step 2, point 4, the previous 'FFPE DNA Repair Buffer' and 'Ultra II End Prep Reaction Buffer' were removed from the MinION/GridION and PromethION branches, as it is preferable to use the latest 'FFPE DNA Repair Buffer v2'. And in step 6, the following was added to all device arms: 'Then cool down to between 4°C and 20°C on the thermal cycler or place the samples on ice.'. - In section 5, 'Priming and loading the MinION and GridION Flow Cell' and 'Flongle Flow Cell loading', an important note was added to the bottom of the section stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device. - For the PromethION branch, the step for removing the PromethION Flow Cell out of the fridge was moved to the beginning of the section on 'Priming and loading the PromethION Flow Cell' to enable the flow cell to reach room temperature while the flow cell priming mix is prepared. And an optional action of performing a flow cell check was included. - In section 6, 'Data acquisition and basecalling', 'To start a sequencing run in MinKNOW', the sequencing kit was specified in point 3, 'Select the Ligation Sequencing Kit V14 (SQK-LSK114) on the Kit page'. And the section on 'Duplex basecalling' was removed as it is no longer applicable. - For the Flongle branch, the sections on 'Flow cell deterioration/saturation' and 'Flongle Sequencing Expansion' were edited. - The video link for priming and loading a Flongle Flow Cell was updated in section 5, step 4. - Screenshots were removed in section 6, 'Data acquisition and basecalling'.
5/22/2025GDE_9161_v114_revZ_08May2025日本Flongle
8th May 2025 - In 'Overview', library preparation time was increased to ~65 min to align with the Table and Figure in 'Steps in the sequencing workflow' where the priming and loading of flow cell was increased from 5 min to 10 min. - In section 1, 'Overview of the protocol', 'Introduction to the Ligation Sequencing Kit V14 (SQK-LSK114) protocol', the snippet setting was changed from being a Tip (green box) to Information. - In section 3, 'DNA repair and end-prep, step 2, point 4, the previous 'FFPE DNA Repair Buffer' and 'Ultra II End Prep Reaction Buffer' were removed from the MinION/GridION and PromethION branches, as it is preferable to use the latest 'FFPE DNA Repair Buffer v2'. And in step 6, the following was added to all device arms: 'Then cool down to between 4°C and 20°C on the thermal cycler or place the samples on ice.'. - In section 5, 'Priming and loading the MinION and GridION Flow Cell' and 'Flongle Flow Cell loading', an important note was added to the bottom of the section stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device. - For the PromethION branch, the step for removing the PromethION Flow Cell out of the fridge was moved to the beginning of the section on 'Priming and loading the PromethION Flow Cell' to enable the flow cell to reach room temperature while the flow cell priming mix is prepared. And an optional action of performing a flow cell check was included. - In section 6, 'Data acquisition and basecalling', 'To start a sequencing run in MinKNOW', the sequencing kit was specified in point 3, 'Select the Ligation Sequencing Kit V14 (SQK-LSK114) on the Kit page'. And the section on 'Duplex basecalling' was removed as it is no longer applicable. - For the Flongle branch, the sections on 'Flow cell deterioration/saturation' and 'Flongle Sequencing Expansion' were edited. - The video link for priming and loading a Flongle Flow Cell was updated in section 5, step 4. - Screenshots were removed in section 6, 'Data acquisition and basecalling'.
5/22/2025GDE_9161_v114_revZ_08May2025日本GridION
8th May 2025 - In 'Overview', library preparation time was increased to ~65 min to align with the Table and Figure in 'Steps in the sequencing workflow' where the priming and loading of flow cell was increased from 5 min to 10 min. - In section 1, 'Overview of the protocol', 'Introduction to the Ligation Sequencing Kit V14 (SQK-LSK114) protocol', the snippet setting was changed from being a Tip (green box) to Information. - In section 3, 'DNA repair and end-prep, step 2, point 4, the previous 'FFPE DNA Repair Buffer' and 'Ultra II End Prep Reaction Buffer' were removed from the MinION/GridION and PromethION branches, as it is preferable to use the latest 'FFPE DNA Repair Buffer v2'. And in step 6, the following was added to all device arms: 'Then cool down to between 4°C and 20°C on the thermal cycler or place the samples on ice.'. - In section 5, 'Priming and loading the MinION and GridION Flow Cell' and 'Flongle Flow Cell loading', an important note was added to the bottom of the section stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device. - For the PromethION branch, the step for removing the PromethION Flow Cell out of the fridge was moved to the beginning of the section on 'Priming and loading the PromethION Flow Cell' to enable the flow cell to reach room temperature while the flow cell priming mix is prepared. And an optional action of performing a flow cell check was included. - In section 6, 'Data acquisition and basecalling', 'To start a sequencing run in MinKNOW', the sequencing kit was specified in point 3, 'Select the Ligation Sequencing Kit V14 (SQK-LSK114) on the Kit page'. And the section on 'Duplex basecalling' was removed as it is no longer applicable. - For the Flongle branch, the sections on 'Flow cell deterioration/saturation' and 'Flongle Sequencing Expansion' were edited. - The video link for priming and loading a Flongle Flow Cell was updated in section 5, step 4. - Screenshots were removed in section 6, 'Data acquisition and basecalling'.
5/22/2025GDE_9161_v114_revZ_08May2025日本MinION
8th May 2025 - In 'Overview', library preparation time was increased to ~65 min to align with the Table and Figure in 'Steps in the sequencing workflow' where the priming and loading of flow cell was increased from 5 min to 10 min. - In section 1, 'Overview of the protocol', 'Introduction to the Ligation Sequencing Kit V14 (SQK-LSK114) protocol', the snippet setting was changed from being a Tip (green box) to Information. - In section 3, 'DNA repair and end-prep, step 2, point 4, the previous 'FFPE DNA Repair Buffer' and 'Ultra II End Prep Reaction Buffer' were removed from the MinION/GridION and PromethION branches, as it is preferable to use the latest 'FFPE DNA Repair Buffer v2'. And in step 6, the following was added to all device arms: 'Then cool down to between 4°C and 20°C on the thermal cycler or place the samples on ice.'. - In section 5, 'Priming and loading the MinION and GridION Flow Cell' and 'Flongle Flow Cell loading', an important note was added to the bottom of the section stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device. - For the PromethION branch, the step for removing the PromethION Flow Cell out of the fridge was moved to the beginning of the section on 'Priming and loading the PromethION Flow Cell' to enable the flow cell to reach room temperature while the flow cell priming mix is prepared. And an optional action of performing a flow cell check was included. - In section 6, 'Data acquisition and basecalling', 'To start a sequencing run in MinKNOW', the sequencing kit was specified in point 3, 'Select the Ligation Sequencing Kit V14 (SQK-LSK114) on the Kit page'. And the section on 'Duplex basecalling' was removed as it is no longer applicable. - For the Flongle branch, the sections on 'Flow cell deterioration/saturation' and 'Flongle Sequencing Expansion' were edited. - The video link for priming and loading a Flongle Flow Cell was updated in section 5, step 4. - Screenshots were removed in section 6, 'Data acquisition and basecalling'.
5/22/2025GDE_9161_v114_revZ_08May2025日本PromethION
8th May 2025 - In 'Overview', library preparation time was increased to ~65 min to align with the Table and Figure in 'Steps in the sequencing workflow' where the priming and loading of flow cell was increased from 5 min to 10 min. - In section 1, 'Overview of the protocol', 'Introduction to the Ligation Sequencing Kit V14 (SQK-LSK114) protocol', the snippet setting was changed from being a Tip (green box) to Information. - In section 3, 'DNA repair and end-prep, step 2, point 4, the previous 'FFPE DNA Repair Buffer' and 'Ultra II End Prep Reaction Buffer' were removed from the MinION/GridION and PromethION branches, as it is preferable to use the latest 'FFPE DNA Repair Buffer v2'. And in step 6, the following was added to all device arms: 'Then cool down to between 4°C and 20°C on the thermal cycler or place the samples on ice.'. - In section 5, 'Priming and loading the MinION and GridION Flow Cell' and 'Flongle Flow Cell loading', an important note was added to the bottom of the section stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device. - For the PromethION branch, the step for removing the PromethION Flow Cell out of the fridge was moved to the beginning of the section on 'Priming and loading the PromethION Flow Cell' to enable the flow cell to reach room temperature while the flow cell priming mix is prepared. And an optional action of performing a flow cell check was included. - In section 6, 'Data acquisition and basecalling', 'To start a sequencing run in MinKNOW', the sequencing kit was specified in point 3, 'Select the Ligation Sequencing Kit V14 (SQK-LSK114) on the Kit page'. And the section on 'Duplex basecalling' was removed as it is no longer applicable. - For the Flongle branch, the sections on 'Flow cell deterioration/saturation' and 'Flongle Sequencing Expansion' were edited. - The video link for priming and loading a Flongle Flow Cell was updated in section 5, step 4. - Screenshots were removed in section 6, 'Data acquisition and basecalling'.
5/22/2025GDE_9161_v114_revZ_08May2025中文MinION
8th May 2025 - In 'Overview', library preparation time was increased to ~65 min to align with the Table and Figure in 'Steps in the sequencing workflow' where the priming and loading of flow cell was increased from 5 min to 10 min. - In section 1, 'Overview of the protocol', 'Introduction to the Ligation Sequencing Kit V14 (SQK-LSK114) protocol', the snippet setting was changed from being a Tip (green box) to Information. - In section 3, 'DNA repair and end-prep, step 2, point 4, the previous 'FFPE DNA Repair Buffer' and 'Ultra II End Prep Reaction Buffer' were removed from the MinION/GridION and PromethION branches, as it is preferable to use the latest 'FFPE DNA Repair Buffer v2'. And in step 6, the following was added to all device arms: 'Then cool down to between 4°C and 20°C on the thermal cycler or place the samples on ice.'. - In section 5, 'Priming and loading the MinION and GridION Flow Cell' and 'Flongle Flow Cell loading', an important note was added to the bottom of the section stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device. - For the PromethION branch, the step for removing the PromethION Flow Cell out of the fridge was moved to the beginning of the section on 'Priming and loading the PromethION Flow Cell' to enable the flow cell to reach room temperature while the flow cell priming mix is prepared. And an optional action of performing a flow cell check was included. - In section 6, 'Data acquisition and basecalling', 'To start a sequencing run in MinKNOW', the sequencing kit was specified in point 3, 'Select the Ligation Sequencing Kit V14 (SQK-LSK114) on the Kit page'. And the section on 'Duplex basecalling' was removed as it is no longer applicable. - For the Flongle branch, the sections on 'Flow cell deterioration/saturation' and 'Flongle Sequencing Expansion' were edited. - The video link for priming and loading a Flongle Flow Cell was updated in section 5, step 4. - Screenshots were removed in section 6, 'Data acquisition and basecalling'.
5/22/2025GDE_9161_v114_revZ_08May2025中文PromethION
8th May 2025 - In 'Overview', library preparation time was increased to ~65 min to align with the Table and Figure in 'Steps in the sequencing workflow' where the priming and loading of flow cell was increased from 5 min to 10 min. - In section 1, 'Overview of the protocol', 'Introduction to the Ligation Sequencing Kit V14 (SQK-LSK114) protocol', the snippet setting was changed from being a Tip (green box) to Information. - In section 3, 'DNA repair and end-prep, step 2, point 4, the previous 'FFPE DNA Repair Buffer' and 'Ultra II End Prep Reaction Buffer' were removed from the MinION/GridION and PromethION branches, as it is preferable to use the latest 'FFPE DNA Repair Buffer v2'. And in step 6, the following was added to all device arms: 'Then cool down to between 4°C and 20°C on the thermal cycler or place the samples on ice.'. - In section 5, 'Priming and loading the MinION and GridION Flow Cell' and 'Flongle Flow Cell loading', an important note was added to the bottom of the section stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device. - For the PromethION branch, the step for removing the PromethION Flow Cell out of the fridge was moved to the beginning of the section on 'Priming and loading the PromethION Flow Cell' to enable the flow cell to reach room temperature while the flow cell priming mix is prepared. And an optional action of performing a flow cell check was included. - In section 6, 'Data acquisition and basecalling', 'To start a sequencing run in MinKNOW', the sequencing kit was specified in point 3, 'Select the Ligation Sequencing Kit V14 (SQK-LSK114) on the Kit page'. And the section on 'Duplex basecalling' was removed as it is no longer applicable. - For the Flongle branch, the sections on 'Flow cell deterioration/saturation' and 'Flongle Sequencing Expansion' were edited. - The video link for priming and loading a Flongle Flow Cell was updated in section 5, step 4. - Screenshots were removed in section 6, 'Data acquisition and basecalling'.
5/9/2025GDE_9161_v114_revZ_08May2025EnglishFlongle
8th May 2025 - In 'Overview', library preparation time was increased to ~65 min to align with the Table and Figure in 'Steps in the sequencing workflow' where the priming and loading of flow cell was increased from 5 min to 10 min. - In section 1, 'Overview of the protocol', 'Introduction to the Ligation Sequencing Kit V14 (SQK-LSK114) protocol', the snippet setting was changed from being a Tip (green box) to Information. - In section 3, 'DNA repair and end-prep, step 2, point 4, the previous 'FFPE DNA Repair Buffer' and 'Ultra II End Prep Reaction Buffer' were removed from the MinION/GridION and PromethION branches, as it is preferable to use the latest 'FFPE DNA Repair Buffer v2'. And in step 6, the following was added to all device arms: 'Then cool down to between 4°C and 20°C on the thermal cycler or place the samples on ice.'. - In section 5, 'Priming and loading the MinION and GridION Flow Cell' and 'Flongle Flow Cell loading', an important note was added to the bottom of the section stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device. - For the PromethION branch, the step for removing the PromethION Flow Cell out of the fridge was moved to the beginning of the section on 'Priming and loading the PromethION Flow Cell' to enable the flow cell to reach room temperature while the flow cell priming mix is prepared. And an optional action of performing a flow cell check was included. - In section 6, 'Data acquisition and basecalling', 'To start a sequencing run in MinKNOW', the sequencing kit was specified in point 3, 'Select the Ligation Sequencing Kit V14 (SQK-LSK114) on the Kit page'. And the section on 'Duplex basecalling' was removed as it is no longer applicable. - For the Flongle branch, the sections on 'Flow cell deterioration/saturation' and 'Flongle Sequencing Expansion' were edited. - The video link for priming and loading a Flongle Flow Cell was updated in section 5, step 4. - Screenshots were removed in section 6, 'Data acquisition and basecalling'.
5/9/2025GDE_9161_v114_revZ_08May2025EnglishGridION
8th May 2025 - In 'Overview', library preparation time was increased to ~65 min to align with the Table and Figure in 'Steps in the sequencing workflow' where the priming and loading of flow cell was increased from 5 min to 10 min. - In section 1, 'Overview of the protocol', 'Introduction to the Ligation Sequencing Kit V14 (SQK-LSK114) protocol', the snippet setting was changed from being a Tip (green box) to Information. - In section 3, 'DNA repair and end-prep, step 2, point 4, the previous 'FFPE DNA Repair Buffer' and 'Ultra II End Prep Reaction Buffer' were removed from the MinION/GridION and PromethION branches, as it is preferable to use the latest 'FFPE DNA Repair Buffer v2'. And in step 6, the following was added to all device arms: 'Then cool down to between 4°C and 20°C on the thermal cycler or place the samples on ice.'. - In section 5, 'Priming and loading the MinION and GridION Flow Cell' and 'Flongle Flow Cell loading', an important note was added to the bottom of the section stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device. - For the PromethION branch, the step for removing the PromethION Flow Cell out of the fridge was moved to the beginning of the section on 'Priming and loading the PromethION Flow Cell' to enable the flow cell to reach room temperature while the flow cell priming mix is prepared. And an optional action of performing a flow cell check was included. - In section 6, 'Data acquisition and basecalling', 'To start a sequencing run in MinKNOW', the sequencing kit was specified in point 3, 'Select the Ligation Sequencing Kit V14 (SQK-LSK114) on the Kit page'. And the section on 'Duplex basecalling' was removed as it is no longer applicable. - For the Flongle branch, the sections on 'Flow cell deterioration/saturation' and 'Flongle Sequencing Expansion' were edited. - The video link for priming and loading a Flongle Flow Cell was updated in section 5, step 4. - Screenshots were removed in section 6, 'Data acquisition and basecalling'.
5/9/2025GDE_9161_v114_revZ_08May2025EnglishMinION
8th May 2025 - In 'Overview', library preparation time was increased to ~65 min to align with the Table and Figure in 'Steps in the sequencing workflow' where the priming and loading of flow cell was increased from 5 min to 10 min. - In section 1, 'Overview of the protocol', 'Introduction to the Ligation Sequencing Kit V14 (SQK-LSK114) protocol', the snippet setting was changed from being a Tip (green box) to Information. - In section 3, 'DNA repair and end-prep, step 2, point 4, the previous 'FFPE DNA Repair Buffer' and 'Ultra II End Prep Reaction Buffer' were removed from the MinION/GridION and PromethION branches, as it is preferable to use the latest 'FFPE DNA Repair Buffer v2'. And in step 6, the following was added to all device arms: 'Then cool down to between 4°C and 20°C on the thermal cycler or place the samples on ice.'. - In section 5, 'Priming and loading the MinION and GridION Flow Cell' and 'Flongle Flow Cell loading', an important note was added to the bottom of the section stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device. - For the PromethION branch, the step for removing the PromethION Flow Cell out of the fridge was moved to the beginning of the section on 'Priming and loading the PromethION Flow Cell' to enable the flow cell to reach room temperature while the flow cell priming mix is prepared. And an optional action of performing a flow cell check was included. - In section 6, 'Data acquisition and basecalling', 'To start a sequencing run in MinKNOW', the sequencing kit was specified in point 3, 'Select the Ligation Sequencing Kit V14 (SQK-LSK114) on the Kit page'. And the section on 'Duplex basecalling' was removed as it is no longer applicable. - For the Flongle branch, the sections on 'Flow cell deterioration/saturation' and 'Flongle Sequencing Expansion' were edited. - The video link for priming and loading a Flongle Flow Cell was updated in section 5, step 4. - Screenshots were removed in section 6, 'Data acquisition and basecalling'.
5/9/2025GDE_9161_v114_revZ_08May2025EnglishPromethION
8th May 2025 - In 'Overview', library preparation time was increased to ~65 min to align with the Table and Figure in 'Steps in the sequencing workflow' where the priming and loading of flow cell was increased from 5 min to 10 min. - In section 1, 'Overview of the protocol', 'Introduction to the Ligation Sequencing Kit V14 (SQK-LSK114) protocol', the snippet setting was changed from being a Tip (green box) to Information. - In section 3, 'DNA repair and end-prep, step 2, point 4, the previous 'FFPE DNA Repair Buffer' and 'Ultra II End Prep Reaction Buffer' were removed from the MinION/GridION and PromethION branches, as it is preferable to use the latest 'FFPE DNA Repair Buffer v2'. And in step 6, the following was added to all device arms: 'Then cool down to between 4°C and 20°C on the thermal cycler or place the samples on ice.'. - In section 5, 'Priming and loading the MinION and GridION Flow Cell' and 'Flongle Flow Cell loading', an important note was added to the bottom of the section stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device. - For the PromethION branch, the step for removing the PromethION Flow Cell out of the fridge was moved to the beginning of the section on 'Priming and loading the PromethION Flow Cell' to enable the flow cell to reach room temperature while the flow cell priming mix is prepared. And an optional action of performing a flow cell check was included. - In section 6, 'Data acquisition and basecalling', 'To start a sequencing run in MinKNOW', the sequencing kit was specified in point 3, 'Select the Ligation Sequencing Kit V14 (SQK-LSK114) on the Kit page'. And the section on 'Duplex basecalling' was removed as it is no longer applicable. - For the Flongle branch, the sections on 'Flow cell deterioration/saturation' and 'Flongle Sequencing Expansion' were edited. - The video link for priming and loading a Flongle Flow Cell was updated in section 5, step 4. - Screenshots were removed in section 6, 'Data acquisition and basecalling'.
5/9/2025GDE_9161_v114_revZ_08May2025EspañolMinION
8th May 2025 - In 'Overview', library preparation time was increased to ~65 min to align with the Table and Figure in 'Steps in the sequencing workflow' where the priming and loading of flow cell was increased from 5 min to 10 min. - In section 1, 'Overview of the protocol', 'Introduction to the Ligation Sequencing Kit V14 (SQK-LSK114) protocol', the snippet setting was changed from being a Tip (green box) to Information. - In section 3, 'DNA repair and end-prep, step 2, point 4, the previous 'FFPE DNA Repair Buffer' and 'Ultra II End Prep Reaction Buffer' were removed from the MinION/GridION and PromethION branches, as it is preferable to use the latest 'FFPE DNA Repair Buffer v2'. And in step 6, the following was added to all device arms: 'Then cool down to between 4°C and 20°C on the thermal cycler or place the samples on ice.'. - In section 5, 'Priming and loading the MinION and GridION Flow Cell' and 'Flongle Flow Cell loading', an important note was added to the bottom of the section stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device. - For the PromethION branch, the step for removing the PromethION Flow Cell out of the fridge was moved to the beginning of the section on 'Priming and loading the PromethION Flow Cell' to enable the flow cell to reach room temperature while the flow cell priming mix is prepared. And an optional action of performing a flow cell check was included. - In section 6, 'Data acquisition and basecalling', 'To start a sequencing run in MinKNOW', the sequencing kit was specified in point 3, 'Select the Ligation Sequencing Kit V14 (SQK-LSK114) on the Kit page'. And the section on 'Duplex basecalling' was removed as it is no longer applicable. - For the Flongle branch, the sections on 'Flow cell deterioration/saturation' and 'Flongle Sequencing Expansion' were edited. - The video link for priming and loading a Flongle Flow Cell was updated in section 5, step 4. - Screenshots were removed in section 6, 'Data acquisition and basecalling'.
5/9/2025GDE_9161_v114_revZ_08May2025EspañolPromethION
8th May 2025 - In 'Overview', library preparation time was increased to ~65 min to align with the Table and Figure in 'Steps in the sequencing workflow' where the priming and loading of flow cell was increased from 5 min to 10 min. - In section 1, 'Overview of the protocol', 'Introduction to the Ligation Sequencing Kit V14 (SQK-LSK114) protocol', the snippet setting was changed from being a Tip (green box) to Information. - In section 3, 'DNA repair and end-prep, step 2, point 4, the previous 'FFPE DNA Repair Buffer' and 'Ultra II End Prep Reaction Buffer' were removed from the MinION/GridION and PromethION branches, as it is preferable to use the latest 'FFPE DNA Repair Buffer v2'. And in step 6, the following was added to all device arms: 'Then cool down to between 4°C and 20°C on the thermal cycler or place the samples on ice.'. - In section 5, 'Priming and loading the MinION and GridION Flow Cell' and 'Flongle Flow Cell loading', an important note was added to the bottom of the section stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device. - For the PromethION branch, the step for removing the PromethION Flow Cell out of the fridge was moved to the beginning of the section on 'Priming and loading the PromethION Flow Cell' to enable the flow cell to reach room temperature while the flow cell priming mix is prepared. And an optional action of performing a flow cell check was included. - In section 6, 'Data acquisition and basecalling', 'To start a sequencing run in MinKNOW', the sequencing kit was specified in point 3, 'Select the Ligation Sequencing Kit V14 (SQK-LSK114) on the Kit page'. And the section on 'Duplex basecalling' was removed as it is no longer applicable. - For the Flongle branch, the sections on 'Flow cell deterioration/saturation' and 'Flongle Sequencing Expansion' were edited. - The video link for priming and loading a Flongle Flow Cell was updated in section 5, step 4. - Screenshots were removed in section 6, 'Data acquisition and basecalling'.
5/9/2025GDE_9161_v114_revZ_08May2025日本Flongle
8th May 2025 - In 'Overview', library preparation time was increased to ~65 min to align with the Table and Figure in 'Steps in the sequencing workflow' where the priming and loading of flow cell was increased from 5 min to 10 min. - In section 1, 'Overview of the protocol', 'Introduction to the Ligation Sequencing Kit V14 (SQK-LSK114) protocol', the snippet setting was changed from being a Tip (green box) to Information. - In section 3, 'DNA repair and end-prep, step 2, point 4, the previous 'FFPE DNA Repair Buffer' and 'Ultra II End Prep Reaction Buffer' were removed from the MinION/GridION and PromethION branches, as it is preferable to use the latest 'FFPE DNA Repair Buffer v2'. And in step 6, the following was added to all device arms: 'Then cool down to between 4°C and 20°C on the thermal cycler or place the samples on ice.'. - In section 5, 'Priming and loading the MinION and GridION Flow Cell' and 'Flongle Flow Cell loading', an important note was added to the bottom of the section stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device. - For the PromethION branch, the step for removing the PromethION Flow Cell out of the fridge was moved to the beginning of the section on 'Priming and loading the PromethION Flow Cell' to enable the flow cell to reach room temperature while the flow cell priming mix is prepared. And an optional action of performing a flow cell check was included. - In section 6, 'Data acquisition and basecalling', 'To start a sequencing run in MinKNOW', the sequencing kit was specified in point 3, 'Select the Ligation Sequencing Kit V14 (SQK-LSK114) on the Kit page'. And the section on 'Duplex basecalling' was removed as it is no longer applicable. - For the Flongle branch, the sections on 'Flow cell deterioration/saturation' and 'Flongle Sequencing Expansion' were edited. - The video link for priming and loading a Flongle Flow Cell was updated in section 5, step 4. - Screenshots were removed in section 6, 'Data acquisition and basecalling'.
5/9/2025GDE_9161_v114_revZ_08May2025日本GridION
8th May 2025 - In 'Overview', library preparation time was increased to ~65 min to align with the Table and Figure in 'Steps in the sequencing workflow' where the priming and loading of flow cell was increased from 5 min to 10 min. - In section 1, 'Overview of the protocol', 'Introduction to the Ligation Sequencing Kit V14 (SQK-LSK114) protocol', the snippet setting was changed from being a Tip (green box) to Information. - In section 3, 'DNA repair and end-prep, step 2, point 4, the previous 'FFPE DNA Repair Buffer' and 'Ultra II End Prep Reaction Buffer' were removed from the MinION/GridION and PromethION branches, as it is preferable to use the latest 'FFPE DNA Repair Buffer v2'. And in step 6, the following was added to all device arms: 'Then cool down to between 4°C and 20°C on the thermal cycler or place the samples on ice.'. - In section 5, 'Priming and loading the MinION and GridION Flow Cell' and 'Flongle Flow Cell loading', an important note was added to the bottom of the section stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device. - For the PromethION branch, the step for removing the PromethION Flow Cell out of the fridge was moved to the beginning of the section on 'Priming and loading the PromethION Flow Cell' to enable the flow cell to reach room temperature while the flow cell priming mix is prepared. And an optional action of performing a flow cell check was included. - In section 6, 'Data acquisition and basecalling', 'To start a sequencing run in MinKNOW', the sequencing kit was specified in point 3, 'Select the Ligation Sequencing Kit V14 (SQK-LSK114) on the Kit page'. And the section on 'Duplex basecalling' was removed as it is no longer applicable. - For the Flongle branch, the sections on 'Flow cell deterioration/saturation' and 'Flongle Sequencing Expansion' were edited. - The video link for priming and loading a Flongle Flow Cell was updated in section 5, step 4. - Screenshots were removed in section 6, 'Data acquisition and basecalling'.
5/9/2025GDE_9161_v114_revZ_08May2025日本MinION
8th May 2025 - In 'Overview', library preparation time was increased to ~65 min to align with the Table and Figure in 'Steps in the sequencing workflow' where the priming and loading of flow cell was increased from 5 min to 10 min. - In section 1, 'Overview of the protocol', 'Introduction to the Ligation Sequencing Kit V14 (SQK-LSK114) protocol', the snippet setting was changed from being a Tip (green box) to Information. - In section 3, 'DNA repair and end-prep, step 2, point 4, the previous 'FFPE DNA Repair Buffer' and 'Ultra II End Prep Reaction Buffer' were removed from the MinION/GridION and PromethION branches, as it is preferable to use the latest 'FFPE DNA Repair Buffer v2'. And in step 6, the following was added to all device arms: 'Then cool down to between 4°C and 20°C on the thermal cycler or place the samples on ice.'. - In section 5, 'Priming and loading the MinION and GridION Flow Cell' and 'Flongle Flow Cell loading', an important note was added to the bottom of the section stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device. - For the PromethION branch, the step for removing the PromethION Flow Cell out of the fridge was moved to the beginning of the section on 'Priming and loading the PromethION Flow Cell' to enable the flow cell to reach room temperature while the flow cell priming mix is prepared. And an optional action of performing a flow cell check was included. - In section 6, 'Data acquisition and basecalling', 'To start a sequencing run in MinKNOW', the sequencing kit was specified in point 3, 'Select the Ligation Sequencing Kit V14 (SQK-LSK114) on the Kit page'. And the section on 'Duplex basecalling' was removed as it is no longer applicable. - For the Flongle branch, the sections on 'Flow cell deterioration/saturation' and 'Flongle Sequencing Expansion' were edited. - The video link for priming and loading a Flongle Flow Cell was updated in section 5, step 4. - Screenshots were removed in section 6, 'Data acquisition and basecalling'.
5/9/2025GDE_9161_v114_revZ_08May2025日本PromethION
8th May 2025 - In 'Overview', library preparation time was increased to ~65 min to align with the Table and Figure in 'Steps in the sequencing workflow' where the priming and loading of flow cell was increased from 5 min to 10 min. - In section 1, 'Overview of the protocol', 'Introduction to the Ligation Sequencing Kit V14 (SQK-LSK114) protocol', the snippet setting was changed from being a Tip (green box) to Information. - In section 3, 'DNA repair and end-prep, step 2, point 4, the previous 'FFPE DNA Repair Buffer' and 'Ultra II End Prep Reaction Buffer' were removed from the MinION/GridION and PromethION branches, as it is preferable to use the latest 'FFPE DNA Repair Buffer v2'. And in step 6, the following was added to all device arms: 'Then cool down to between 4°C and 20°C on the thermal cycler or place the samples on ice.'. - In section 5, 'Priming and loading the MinION and GridION Flow Cell' and 'Flongle Flow Cell loading', an important note was added to the bottom of the section stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device. - For the PromethION branch, the step for removing the PromethION Flow Cell out of the fridge was moved to the beginning of the section on 'Priming and loading the PromethION Flow Cell' to enable the flow cell to reach room temperature while the flow cell priming mix is prepared. And an optional action of performing a flow cell check was included. - In section 6, 'Data acquisition and basecalling', 'To start a sequencing run in MinKNOW', the sequencing kit was specified in point 3, 'Select the Ligation Sequencing Kit V14 (SQK-LSK114) on the Kit page'. And the section on 'Duplex basecalling' was removed as it is no longer applicable. - For the Flongle branch, the sections on 'Flow cell deterioration/saturation' and 'Flongle Sequencing Expansion' were edited. - The video link for priming and loading a Flongle Flow Cell was updated in section 5, step 4. - Screenshots were removed in section 6, 'Data acquisition and basecalling'.
5/9/2025GDE_9161_v114_revZ_08May2025中文MinION
8th May 2025 - In 'Overview', library preparation time was increased to ~65 min to align with the Table and Figure in 'Steps in the sequencing workflow' where the priming and loading of flow cell was increased from 5 min to 10 min. - In section 1, 'Overview of the protocol', 'Introduction to the Ligation Sequencing Kit V14 (SQK-LSK114) protocol', the snippet setting was changed from being a Tip (green box) to Information. - In section 3, 'DNA repair and end-prep, step 2, point 4, the previous 'FFPE DNA Repair Buffer' and 'Ultra II End Prep Reaction Buffer' were removed from the MinION/GridION and PromethION branches, as it is preferable to use the latest 'FFPE DNA Repair Buffer v2'. And in step 6, the following was added to all device arms: 'Then cool down to between 4°C and 20°C on the thermal cycler or place the samples on ice.'. - In section 5, 'Priming and loading the MinION and GridION Flow Cell' and 'Flongle Flow Cell loading', an important note was added to the bottom of the section stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device. - For the PromethION branch, the step for removing the PromethION Flow Cell out of the fridge was moved to the beginning of the section on 'Priming and loading the PromethION Flow Cell' to enable the flow cell to reach room temperature while the flow cell priming mix is prepared. And an optional action of performing a flow cell check was included. - In section 6, 'Data acquisition and basecalling', 'To start a sequencing run in MinKNOW', the sequencing kit was specified in point 3, 'Select the Ligation Sequencing Kit V14 (SQK-LSK114) on the Kit page'. And the section on 'Duplex basecalling' was removed as it is no longer applicable. - For the Flongle branch, the sections on 'Flow cell deterioration/saturation' and 'Flongle Sequencing Expansion' were edited. - The video link for priming and loading a Flongle Flow Cell was updated in section 5, step 4. - Screenshots were removed in section 6, 'Data acquisition and basecalling'.
5/9/2025GDE_9161_v114_revZ_08May2025中文PromethION
8th May 2025 - In 'Overview', library preparation time was increased to ~65 min to align with the Table and Figure in 'Steps in the sequencing workflow' where the priming and loading of flow cell was increased from 5 min to 10 min. - In section 1, 'Overview of the protocol', 'Introduction to the Ligation Sequencing Kit V14 (SQK-LSK114) protocol', the snippet setting was changed from being a Tip (green box) to Information. - In section 3, 'DNA repair and end-prep, step 2, point 4, the previous 'FFPE DNA Repair Buffer' and 'Ultra II End Prep Reaction Buffer' were removed from the MinION/GridION and PromethION branches, as it is preferable to use the latest 'FFPE DNA Repair Buffer v2'. And in step 6, the following was added to all device arms: 'Then cool down to between 4°C and 20°C on the thermal cycler or place the samples on ice.'. - In section 5, 'Priming and loading the MinION and GridION Flow Cell' and 'Flongle Flow Cell loading', an important note was added to the bottom of the section stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device. - For the PromethION branch, the step for removing the PromethION Flow Cell out of the fridge was moved to the beginning of the section on 'Priming and loading the PromethION Flow Cell' to enable the flow cell to reach room temperature while the flow cell priming mix is prepared. And an optional action of performing a flow cell check was included. - In section 6, 'Data acquisition and basecalling', 'To start a sequencing run in MinKNOW', the sequencing kit was specified in point 3, 'Select the Ligation Sequencing Kit V14 (SQK-LSK114) on the Kit page'. And the section on 'Duplex basecalling' was removed as it is no longer applicable. - For the Flongle branch, the sections on 'Flow cell deterioration/saturation' and 'Flongle Sequencing Expansion' were edited. - The video link for priming and loading a Flongle Flow Cell was updated in section 5, step 4. - Screenshots were removed in section 6, 'Data acquisition and basecalling'.
1/30/2025GDE_9161_v114_revY_30Jan2025EnglishFlongle
30th January 2025 In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
1/30/2025GDE_9161_v114_revY_30Jan2025EnglishGridION
30th January 2025 In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
1/30/2025GDE_9161_v114_revY_30Jan2025EnglishMinION
30th January 2025 In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
1/30/2025GDE_9161_v114_revY_30Jan2025EnglishPromethION
30th January 2025 In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
1/30/2025GDE_9161_v114_revY_30Jan2025EspañolFlongle
30th January 2025 In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
1/30/2025GDE_9161_v114_revY_30Jan2025EspañolGridION
30th January 2025 In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
1/30/2025GDE_9161_v114_revY_30Jan2025EspañolMinION
30th January 2025 In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
1/30/2025GDE_9161_v114_revY_30Jan2025EspañolPromethION
30th January 2025 In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
1/30/2025GDE_9161_v114_revY_30Jan2025日本Flongle
30th January 2025 In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
1/30/2025GDE_9161_v114_revY_30Jan2025日本GridION
30th January 2025 In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
1/30/2025GDE_9161_v114_revY_30Jan2025日本MinION
30th January 2025 In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
1/30/2025GDE_9161_v114_revY_30Jan2025日本PromethION
30th January 2025 In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
1/30/2025GDE_9161_v114_revY_30Jan2025中文Flongle
30th January 2025 In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
1/30/2025GDE_9161_v114_revY_30Jan2025中文GridION
30th January 2025 In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
1/30/2025GDE_9161_v114_revY_30Jan2025中文MinION
30th January 2025 In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
1/30/2025GDE_9161_v114_revY_30Jan2025中文PromethION
30th January 2025 In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
1/30/2025GDE_9161_v114_revY_30Jan2025EnglishFlongle
30th January 2025 In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
1/30/2025GDE_9161_v114_revY_30Jan2025EnglishGridION
30th January 2025 In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
1/30/2025GDE_9161_v114_revY_30Jan2025EnglishMinION
30th January 2025 In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
1/30/2025GDE_9161_v114_revY_30Jan2025EnglishPromethION
30th January 2025 In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
1/30/2025GDE_9161_v114_revY_30Jan2025EspañolFlongle
30th January 2025 In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
1/30/2025GDE_9161_v114_revY_30Jan2025EspañolGridION
30th January 2025 In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
1/30/2025GDE_9161_v114_revY_30Jan2025EspañolMinION
30th January 2025 In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
1/30/2025GDE_9161_v114_revY_30Jan2025EspañolPromethION
30th January 2025 In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
1/30/2025GDE_9161_v114_revY_30Jan2025日本Flongle
30th January 2025 In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
1/30/2025GDE_9161_v114_revY_30Jan2025日本GridION
30th January 2025 In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
1/30/2025GDE_9161_v114_revY_30Jan2025日本MinION
30th January 2025 In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
1/30/2025GDE_9161_v114_revY_30Jan2025日本PromethION
30th January 2025 In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
1/30/2025GDE_9161_v114_revY_30Jan2025中文Flongle
30th January 2025 In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
1/30/2025GDE_9161_v114_revY_30Jan2025中文GridION
30th January 2025 In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
1/30/2025GDE_9161_v114_revY_30Jan2025中文MinION
30th January 2025 In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
1/30/2025GDE_9161_v114_revY_30Jan2025中文PromethION
30th January 2025 In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
12/13/2024GDE_9161_v114_revX_13Dec2024EnglishFlongle
13 December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for EPI2ME in "Downstream analysis". - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/13/2024GDE_9161_v114_revX_13Dec2024EnglishGridION
13 December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for EPI2ME in "Downstream analysis". - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/13/2024GDE_9161_v114_revX_13Dec2024EnglishMinION
13 December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for EPI2ME in "Downstream analysis". - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/13/2024GDE_9161_v114_revX_13Dec2024EnglishPromethION
13 December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for EPI2ME in "Downstream analysis". - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/13/2024GDE_9161_v114_revX_13Dec2024EspañolFlongle
13 December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for EPI2ME in "Downstream analysis". - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/13/2024GDE_9161_v114_revX_13Dec2024EspañolGridION
13 December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for EPI2ME in "Downstream analysis". - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/13/2024GDE_9161_v114_revX_13Dec2024EspañolMinION
13 December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for EPI2ME in "Downstream analysis". - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/13/2024GDE_9161_v114_revX_13Dec2024EspañolPromethION
13 December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for EPI2ME in "Downstream analysis". - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/13/2024GDE_9161_v114_revX_13Dec2024日本Flongle
13 December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for EPI2ME in "Downstream analysis". - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/13/2024GDE_9161_v114_revX_13Dec2024日本GridION
13 December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for EPI2ME in "Downstream analysis". - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/13/2024GDE_9161_v114_revX_13Dec2024日本MinION
13 December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for EPI2ME in "Downstream analysis". - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/13/2024GDE_9161_v114_revX_13Dec2024日本PromethION
13 December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for EPI2ME in "Downstream analysis". - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/13/2024GDE_9161_v114_revX_13Dec2024中文Flongle
13 December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for EPI2ME in "Downstream analysis". - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/13/2024GDE_9161_v114_revX_13Dec2024中文GridION
13 December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for EPI2ME in "Downstream analysis". - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/13/2024GDE_9161_v114_revX_13Dec2024中文MinION
13 December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for EPI2ME in "Downstream analysis". - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/13/2024GDE_9161_v114_revX_13Dec2024中文PromethION
13 December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for EPI2ME in "Downstream analysis". - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/2/2024GDE_9161_v114_revW_29Jun2022EnglishFlongle
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
12/2/2024GDE_9161_v114_revW_29Jun2022EnglishGridION
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
12/2/2024GDE_9161_v114_revW_29Jun2022EnglishMinION
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
12/2/2024GDE_9161_v114_revW_29Jun2022EnglishPromethION
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
12/2/2024GDE_9161_v114_revW_29Jun2022EspañolFlongle
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
12/2/2024GDE_9161_v114_revW_29Jun2022EspañolGridION
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
12/2/2024GDE_9161_v114_revW_29Jun2022EspañolMinION
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
12/2/2024GDE_9161_v114_revW_29Jun2022EspañolPromethION
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
12/2/2024GDE_9161_v114_revW_29Jun2022日本Flongle
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
12/2/2024GDE_9161_v114_revW_29Jun2022日本GridION
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
12/2/2024GDE_9161_v114_revW_29Jun2022日本MinION
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
12/2/2024GDE_9161_v114_revW_29Jun2022日本PromethION
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
12/2/2024GDE_9161_v114_revW_29Jun2022中文Flongle
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
12/2/2024GDE_9161_v114_revW_29Jun2022中文GridION
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
12/2/2024GDE_9161_v114_revW_29Jun2022中文MinION
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
12/2/2024GDE_9161_v114_revW_29Jun2022中文PromethION
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
12/2/2024GDE_9161_v114_revW_29Jun2022EnglishFlongle
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
12/2/2024GDE_9161_v114_revW_29Jun2022EnglishGridION
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
12/2/2024GDE_9161_v114_revW_29Jun2022EnglishMinION
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
12/2/2024GDE_9161_v114_revW_29Jun2022EnglishPromethION
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
12/2/2024GDE_9161_v114_revW_29Jun2022EspañolFlongle
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
12/2/2024GDE_9161_v114_revW_29Jun2022EspañolGridION
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
12/2/2024GDE_9161_v114_revW_29Jun2022EspañolMinION
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
12/2/2024GDE_9161_v114_revW_29Jun2022EspañolPromethION
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
12/2/2024GDE_9161_v114_revW_29Jun2022中文Flongle
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
12/2/2024GDE_9161_v114_revW_29Jun2022中文GridION
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
12/2/2024GDE_9161_v114_revW_29Jun2022中文MinION
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
12/2/2024GDE_9161_v114_revW_29Jun2022中文PromethION
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
11/27/2024GDE_9161_v114_revW_29Jun2022EnglishFlongle
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
11/27/2024GDE_9161_v114_revW_29Jun2022EnglishGridION
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
11/27/2024GDE_9161_v114_revW_29Jun2022EnglishMinION
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
11/27/2024GDE_9161_v114_revW_29Jun2022EnglishPromethION
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
11/27/2024GDE_9161_v114_revW_29Jun2022EspañolFlongle
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
11/27/2024GDE_9161_v114_revW_29Jun2022EspañolGridION
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
11/27/2024GDE_9161_v114_revW_29Jun2022EspañolMinION
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
11/27/2024GDE_9161_v114_revW_29Jun2022EspañolPromethION
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
11/27/2024GDE_9161_v114_revW_29Jun2022中文Flongle
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
11/27/2024GDE_9161_v114_revW_29Jun2022中文GridION
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
11/27/2024GDE_9161_v114_revW_29Jun2022中文MinION
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
11/27/2024GDE_9161_v114_revW_29Jun2022中文PromethION
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
11/13/2024GDE_9161_v114_revW_29Jun2022EnglishFlongle
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
11/13/2024GDE_9161_v114_revW_29Jun2022EnglishGridION
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
11/13/2024GDE_9161_v114_revW_29Jun2022EnglishMinION
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
11/13/2024GDE_9161_v114_revW_29Jun2022EnglishPromethION
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
11/13/2024GDE_9161_v114_revW_29Jun2022EspañolFlongle
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
11/13/2024GDE_9161_v114_revW_29Jun2022EspañolGridION
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
11/13/2024GDE_9161_v114_revW_29Jun2022EspañolMinION
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
11/13/2024GDE_9161_v114_revW_29Jun2022EspañolPromethION
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
11/13/2024GDE_9161_v114_revW_29Jun2022中文Flongle
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
11/13/2024GDE_9161_v114_revW_29Jun2022中文GridION
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
11/13/2024GDE_9161_v114_revW_29Jun2022中文MinION
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
11/13/2024GDE_9161_v114_revW_29Jun2022中文PromethION
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
10/1/2024GDE_9161_v114_revW_29Jun2022EnglishFlongle
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
10/1/2024GDE_9161_v114_revW_29Jun2022EnglishGridION
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
10/1/2024GDE_9161_v114_revW_29Jun2022EnglishMinION
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
10/1/2024GDE_9161_v114_revW_29Jun2022EnglishPromethION
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
10/1/2024GDE_9161_v114_revW_29Jun2022EspañolFlongle
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
10/1/2024GDE_9161_v114_revW_29Jun2022EspañolGridION
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
10/1/2024GDE_9161_v114_revW_29Jun2022EspañolMinION
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
10/1/2024GDE_9161_v114_revW_29Jun2022EspañolPromethION
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
10/1/2024GDE_9161_v114_revW_29Jun2022中文Flongle
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
10/1/2024GDE_9161_v114_revW_29Jun2022中文GridION
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
10/1/2024GDE_9161_v114_revW_29Jun2022中文MinION
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
10/1/2024GDE_9161_v114_revW_29Jun2022中文PromethION
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
8/21/2024GDE_9161_v114_revW_29Jun2022EnglishFlongle
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
8/21/2024GDE_9161_v114_revW_29Jun2022EnglishGridION
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
8/21/2024GDE_9161_v114_revW_29Jun2022EnglishMinION
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
8/21/2024GDE_9161_v114_revW_29Jun2022EnglishPromethION
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
8/21/2024GDE_9161_v114_revW_29Jun2022EspañolFlongle
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
8/21/2024GDE_9161_v114_revW_29Jun2022EspañolGridION
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
8/21/2024GDE_9161_v114_revW_29Jun2022EspañolMinION
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
8/21/2024GDE_9161_v114_revW_29Jun2022EspañolPromethION
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
8/21/2024GDE_9161_v114_revW_29Jun2022中文Flongle
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
8/21/2024GDE_9161_v114_revW_29Jun2022中文GridION
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
8/21/2024GDE_9161_v114_revW_29Jun2022中文MinION
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
8/21/2024GDE_9161_v114_revW_29Jun2022中文PromethION
21st August 2024 - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
August 21st, 2024GDE_9161_v114_revV_30Jul2024EnglishFlongle Download 30th July 2024 - Changed reagent order addition in Library preparation/Adapter ligation and clean-up; Ligation Adapter (LA) added before Ligation Buffer (LB) and NEBNext Quick T4 Ligase 8th December 2023 - Updated the protocol to a slimmed down template.
August 21st, 2024GDE_9161_v114_revV_30Jul2024EnglishMinion Download 30th July 2024 - Changed reagent order addition in Library preparation/Adapter ligation and clean-up; Ligation Adapter (LA) added before Ligation Buffer (LB) and NEBNext Quick T4 Ligase 8th December 2023 - Updated the protocol to a slimmed down template.
August 21st, 2024GDE_9161_v114_revV_30Jul2024EnglishPromethion Download 30th July 2024 - Changed reagent order addition in Library preparation/Adapter ligation and clean-up; Ligation Adapter (LA) added before Ligation Buffer (LB) and NEBNext Quick T4 Ligase 8th December 2023 - Updated the protocol to a slimmed down template.
August 21st, 2024GDE_9161_v114_revV_30Jul2024EnglishGridion Download 30th July 2024 - Changed reagent order addition in Library preparation/Adapter ligation and clean-up; Ligation Adapter (LA) added before Ligation Buffer (LB) and NEBNext Quick T4 Ligase 8th December 2023 - Updated the protocol to a slimmed down template.
July 30th, 2024GDE_9161_v114_revU_29Jun2022EnglishFlongle Download 8th December 2023 - Updated the protocol to a slimmed down template.
July 30th, 2024GDE_9161_v114_revU_29Jun2022EnglishMinion Download 8th December 2023 - Updated the protocol to a slimmed down template.
July 30th, 2024GDE_9161_v114_revU_29Jun2022EnglishPromethion Download 8th December 2023 - Updated the protocol to a slimmed down template.
July 30th, 2024GDE_9161_v114_revU_29Jun2022EnglishGridion Download 8th December 2023 - Updated the protocol to a slimmed down template.
December 8th, 2023GDE_9161_v114_revT_29Jun2022EnglishFlongle Download 4th October 2023 - Updated the format of the final step in the "Adapter ligation and clean-up" step for the checklist
December 8th, 2023GDE_9161_v114_revT_29Jun2022EnglishMinion Download 4th October 2023 - Updated the format of the final step in the "Adapter ligation and clean-up" step for the checklist
December 8th, 2023GDE_9161_v114_revT_29Jun2022EnglishPromethion Download 4th October 2023 - Updated the format of the final step in the "Adapter ligation and clean-up" step for the checklist
December 8th, 2023GDE_9161_v114_revT_29Jun2022EnglishGridion Download 4th October 2023 - Updated the format of the final step in the "Adapter ligation and clean-up" step for the checklist
October 4th, 2023GDE_9161_v114_revS_29Jun2022EnglishFlongle Download 27th September 2023 - Correct error in an equipment and consumable snippet regarding input recommendation for the library preparation
October 4th, 2023GDE_9161_v114_revS_29Jun2022EnglishMinion Download 27th September 2023 - Correct error in an equipment and consumable snippet regarding input recommendation for the library preparation
October 4th, 2023GDE_9161_v114_revS_29Jun2022EnglishPromethion Download 27th September 2023 - Correct error in an equipment and consumable snippet regarding input recommendation for the library preparation
October 4th, 2023GDE_9161_v114_revS_29Jun2022EnglishGridion Download 27th September 2023 - Correct error in an equipment and consumable snippet regarding input recommendation for the library preparation
September 27th, 2023GDE_9161_v114_revQ_29Jun2022EnglishFlongle Download 19th September 2023 - Updated flow cell loading instructions to include light shield installation - Updated flow cell loading recommendations in the "Adapter ligation and clean-up" step. - Update the equipment and consumables section to only be relevant for high molecular weight DNA
September 27th, 2023GDE_9161_v114_revQ_29Jun2022EnglishMinion Download 19th September 2023 - Updated flow cell loading instructions to include light shield installation - Updated flow cell loading recommendations in the "Adapter ligation and clean-up" step. - Update the equipment and consumables section to only be relevant for high molecular weight DNA
September 27th, 2023GDE_9161_v114_revQ_29Jun2022EnglishPromethion Download 19th September 2023 - Updated flow cell loading instructions to include light shield installation - Updated flow cell loading recommendations in the "Adapter ligation and clean-up" step. - Update the equipment and consumables section to only be relevant for high molecular weight DNA
September 27th, 2023GDE_9161_v114_revQ_29Jun2022EnglishGridion Download 19th September 2023 - Updated flow cell loading instructions to include light shield installation - Updated flow cell loading recommendations in the "Adapter ligation and clean-up" step. - Update the equipment and consumables section to only be relevant for high molecular weight DNA
September 19th, 2023GDE_9161_v114_revP_29Jun2022EnglishFlongle Download 1st August 2023 - Kit contents layout has been updated - In priming and loading the flow cell, the flow cell priming mix instructions have been updated
September 19th, 2023GDE_9161_v114_revP_29Jun2022EnglishMinion Download 1st August 2023 - Kit contents layout has been updated - In priming and loading the flow cell, the flow cell priming mix instructions have been updated
September 19th, 2023GDE_9161_v114_revP_29Jun2022EnglishPromethion Download 1st August 2023 - Kit contents layout has been updated - In priming and loading the flow cell, the flow cell priming mix instructions have been updated
September 19th, 2023GDE_9161_v114_revP_29Jun2022EnglishGridion Download 1st August 2023 - Kit contents layout has been updated - In priming and loading the flow cell, the flow cell priming mix instructions have been updated
August 1st, 2023GDE_9161_v114_revO_29Jun2022EnglishFlongle Download May 2023 - Added flow cell priming mix volumes to the "Priming and loading" steps - This kit has moved to released phase.
August 1st, 2023GDE_9161_v114_revO_29Jun2022EnglishMinion Download May 2023 - Added flow cell priming mix volumes to the "Priming and loading" steps - This kit has moved to released phase.
August 1st, 2023GDE_9161_v114_revO_29Jun2022EnglishPromethion Download May 2023 - Added flow cell priming mix volumes to the "Priming and loading" steps - This kit has moved to released phase.
August 1st, 2023GDE_9161_v114_revO_29Jun2022EnglishGridion Download May 2023 - Added flow cell priming mix volumes to the "Priming and loading" steps - This kit has moved to released phase.
April 28th, 2023GDE_9161_v114_revN_29Jun2022EnglishFlongle Download April 2023 - Added flow cell priming mix volumes to the "Priming and loading" steps
April 28th, 2023GDE_9161_v114_revN_29Jun2022EnglishMinion Download April 2023 - Added flow cell priming mix volumes to the "Priming and loading" steps
April 28th, 2023GDE_9161_v114_revN_29Jun2022EnglishPromethion Download April 2023 - Added flow cell priming mix volumes to the "Priming and loading" steps
April 28th, 2023GDE_9161_v114_revN_29Jun2022EnglishGridion Download April 2023 - Added flow cell priming mix volumes to the "Priming and loading" steps
March 15th, 2023GDE_9161_v114_revM_29Jun2022EnglishFlongle Download March 2023: - Updated the "Sequencing and data analysis" section to include further details about duplex basecalling
March 15th, 2023GDE_9161_v114_revM_29Jun2022EnglishMinion Download March 2023: - Updated the "Sequencing and data analysis" section to include further details about duplex basecalling
March 15th, 2023GDE_9161_v114_revM_29Jun2022EnglishPromethion Download March 2023: - Updated the "Sequencing and data analysis" section to include further details about duplex basecalling
March 15th, 2023GDE_9161_v114_revM_29Jun2022EnglishGridion Download March 2023: - Updated the "Sequencing and data analysis" section to include further details about duplex basecalling
January 6th, 2023GDE_9161_v114_revL_29Jun2022EnglishFlongle Download January 2023 - Changed the "Priming and loading the PrometION flow cell" section to the updated version.
January 6th, 2023GDE_9161_v114_revL_29Jun2022EnglishMinion Download January 2023 - Changed the "Priming and loading the PrometION flow cell" section to the updated version.
January 6th, 2023GDE_9161_v114_revL_29Jun2022EnglishPromethion Download January 2023 - Changed the "Priming and loading the PrometION flow cell" section to the updated version.
January 6th, 2023GDE_9161_v114_revL_29Jun2022EnglishGridion Download January 2023 - Changed the "Priming and loading the PrometION flow cell" section to the updated version.
November 30th, 2022GDE_9161_v114_revK_29Jun2022EnglishFlongle Download November 2022 - Updated kit name to Flongle Sequencing Expansion (EXP-FSE002)
November 30th, 2022GDE_9161_v114_revK_29Jun2022EnglishMinion Download November 2022 - Updated kit name to Flongle Sequencing Expansion (EXP-FSE002)
November 30th, 2022GDE_9161_v114_revK_29Jun2022EnglishPromethion Download November 2022 - Updated kit name to Flongle Sequencing Expansion (EXP-FSE002)
November 30th, 2022GDE_9161_v114_revK_29Jun2022EnglishGridion Download November 2022 - Updated kit name to Flongle Sequencing Expansion (EXP-FSE002)
October 27th, 2022GDE_9161_v114_revJ_29Jun2022EnglishFlongle Download 27th October 2022 - Updated protocol to use 80% ethanol rather than 70% ethanol.
October 27th, 2022GDE_9161_v114_revJ_29Jun2022EnglishMinion Download 27th October 2022 - Updated protocol to use 80% ethanol rather than 70% ethanol.
October 27th, 2022GDE_9161_v114_revJ_29Jun2022EnglishPromethion Download 27th October 2022 - Updated protocol to use 80% ethanol rather than 70% ethanol.
October 27th, 2022GDE_9161_v114_revJ_29Jun2022EnglishGridion Download 27th October 2022 - Updated protocol to use 80% ethanol rather than 70% ethanol.
October 26th, 2022GDE_9161_v114_revI_29Jun2022EnglishFlongle Download 19th October 2022 - Added Flongle branch
October 26th, 2022GDE_9161_v114_revI_29Jun2022EnglishMinion Download 19th October 2022 - Added Flongle branch
October 26th, 2022GDE_9161_v114_revI_29Jun2022EnglishPromethion Download 19th October 2022 - Added Flongle branch
October 26th, 2022GDE_9161_v114_revI_29Jun2022EnglishGridion Download 19th October 2022 - Added Flongle branch
October 4th, 2022GDE_9161_v114_revH_29Jun2022EnglishMinion Download 4th Oct 2022 - Updated kit 14 info sheet snippet and link
October 4th, 2022GDE_9161_v114_revH_29Jun2022EnglishPromethion Download 4th Oct 2022 - Updated kit 14 info sheet snippet and link
October 4th, 2022GDE_9161_v114_revH_29Jun2022EnglishGridion Download 4th Oct 2022 - Updated kit 14 info sheet snippet and link
September 16th, 2022GDE_9161_v114_revG_29Jun2022EnglishMinion Download 18th August 2022 - Updated kit contents image
September 16th, 2022GDE_9161_v114_revG_29Jun2022EnglishPromethion Download 18th August 2022 - Updated kit contents image
September 16th, 2022GDE_9161_v114_revG_29Jun2022EnglishGridion Download 18th August 2022 - Updated kit contents image
August 11th, 2022GDE_9161_v114_revF_29Jun2022EnglishMinion Download 10th August 2022 - Added specific concentration of BSA required for preparing the flow cell priming mix
August 11th, 2022GDE_9161_v114_revF_29Jun2022EnglishPromethion Download 10th August 2022 - Added specific concentration of BSA required for preparing the flow cell priming mix
August 11th, 2022GDE_9161_v114_revF_29Jun2022EnglishGridion Download 10th August 2022 - Added specific concentration of BSA required for preparing the flow cell priming mix
August 10th, 2022GDE_9161_v114_revE_29Jun2022EnglishMinion Download 10th August 2022 - Updated phrasing used to add BSA to the flow cell priming mix and the use of ligation buffer
August 10th, 2022GDE_9161_v114_revE_29Jun2022EnglishPromethion Download 10th August 2022 - Updated phrasing used to add BSA to the flow cell priming mix and the use of ligation buffer
August 10th, 2022GDE_9161_v114_revE_29Jun2022EnglishGridion Download 10th August 2022 - Updated phrasing used to add BSA to the flow cell priming mix and the use of ligation buffer
August 10th, 2022GDE_9161_v114_revD_29Jun2022EnglishMinion Download 9th August 2022 - "Priming and loading the SpotON flow cell" has been updated to include the use of BSA for MinION and GridION flow cells
August 10th, 2022GDE_9161_v114_revD_29Jun2022EnglishPromethion Download 9th August 2022 - "Priming and loading the SpotON flow cell" has been updated to include the use of BSA for MinION and GridION flow cells
August 10th, 2022GDE_9161_v114_revD_29Jun2022EnglishGridion Download 9th August 2022 - "Priming and loading the SpotON flow cell" has been updated to include the use of BSA for MinION and GridION flow cells
July 21st, 2022GDE_9161_v114_revC_29Jun2022EnglishMinion Download 21st July 2022 - Updated flow cell loading recommendations for both MinION/GridION
July 21st, 2022GDE_9161_v114_revC_29Jun2022EnglishPromethion Download 21st July 2022 - Updated flow cell loading recommendations for both MinION/GridION
July 21st, 2022GDE_9161_v114_revC_29Jun2022EnglishGridion Download 21st July 2022 - Updated flow cell loading recommendations for both MinION/GridION
July 21st, 2022GDE_9161_v114_revB_29Jun2022EnglishMinion Download 21st July 2022 - Updated flow cell loading recommendations for both MinION/GridION and PromethION - Edited protocol to be inclusive of both gDNA and amplicon DNA inputs. - Overview edited to reflect change above. - Included "Category" and "Sample type" to reflect change above - Included snippet in "Equipment and consumables" and "DNA repair and end- prep" to clarify difference in DNA sample inputs. - Have made "Materials" more inclusive to both DNA input types. - All relevant snippets should now account for both sample input types.
July 21st, 2022GDE_9161_v114_revB_29Jun2022EnglishPromethion Download 21st July 2022 - Updated flow cell loading recommendations for both MinION/GridION and PromethION - Edited protocol to be inclusive of both gDNA and amplicon DNA inputs. - Overview edited to reflect change above. - Included "Category" and "Sample type" to reflect change above - Included snippet in "Equipment and consumables" and "DNA repair and end- prep" to clarify difference in DNA sample inputs. - Have made "Materials" more inclusive to both DNA input types. - All relevant snippets should now account for both sample input types.
July 21st, 2022GDE_9161_v114_revB_29Jun2022EnglishGridion Download 21st July 2022 - Updated flow cell loading recommendations for both MinION/GridION and PromethION - Edited protocol to be inclusive of both gDNA and amplicon DNA inputs. - Overview edited to reflect change above. - Included "Category" and "Sample type" to reflect change above - Included snippet in "Equipment and consumables" and "DNA repair and end- prep" to clarify difference in DNA sample inputs. - Have made "Materials" more inclusive to both DNA input types. - All relevant snippets should now account for both sample input types.
June 30th, 2022GDE_9161_v114_revA_29Jun2022EnglishMinion Download -
June 30th, 2022GDE_9161_v114_revA_29Jun2022EnglishPromethion Download -
June 30th, 2022GDE_9161_v114_revA_29Jun2022EnglishGridion Download -