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Can I use DNA T4 Ligase instead of Blunt T/A for the native barcode ligation?
Can I use DNA T4 Ligase instead of Blunt T/A for the native barcode ligation?
No. The protocol has been optimised and we have found that Blunt T/A works better for barcode ligation.
We have validated and only recommend the use of all the third-party reagents specified in our protocols.
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